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Spatial kinetics and immune control of murine cytomegalovirus infection in the salivary glands

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  • Catherine M Byrne
  • Ana Citlali Márquez
  • Bing Cai
  • Daniel Coombs
  • Soren Gantt

Abstract

Human cytomegalovirus (HCMV) is the most common congenital infection. Several HCMV vaccines are in development, but none have yet been approved. An understanding of the kinetics of CMV replication and transmission may inform the rational design of vaccines to prevent this infection. The salivary glands (SG) are an important site of sustained CMV replication following primary infection and during viral reactivation from latency. As such, the strength of the immune response in the SG likely influences viral dissemination within and between hosts. To study the relationship between the immune response and viral replication in the SG, and viral dissemination from the SG to other tissues, mice were infected with low doses of murine CMV (MCMV). Following intra-SG inoculation, we characterized the viral and immunological dynamics in the SG, blood, and spleen, and identified organ-specific immune correlates of protection. Using these data, we constructed compartmental mathematical models of MCMV infection. Model fitting to data and analysis indicate the importance of cellular immune responses in different organs and point to a threshold of infection within the SG necessary for the establishment and spread of infection.Author summary: Cytomegalovirus (CMV) is the most common congenital infection and causes an enormous burden of childhood disease. To gain insight into the immune requirements for controlling infection, we used a mouse model to reproduce characteristics of natural CMV infection, employing a low viral inoculum, and delivering the virus to the salivary glands (SG), a key site of CMV replication. Our results provide detailed data on the spatial and temporal spread of infection throughout the body and identify key immune correlates of the control of viral replication. By translating these findings into mechanistic mathematical models, we revealed the importance of organ-specific immune responses, particularly the requirement of TNF-α and IFN-γ to control infection within the salivary glands. Furthermore, our mathematical modeling allowed us to compare known characteristics of human CMV infection related to infection establishment and spread to those predicted in mice, underscoring the suitability of the MCMV model to study its human homologue. These insights provide guidance for developing targeted vaccines to prevent CMV infection and disease.

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  • Catherine M Byrne & Ana Citlali Márquez & Bing Cai & Daniel Coombs & Soren Gantt, 2024. "Spatial kinetics and immune control of murine cytomegalovirus infection in the salivary glands," PLOS Computational Biology, Public Library of Science, vol. 20(8), pages 1-21, August.
    • Handle: RePEc:plo:pcbi00:1011940
    DOI: 10.1371/journal.pcbi.1011940
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    1. King, Aaron A. & Nguyen, Dao & Ionides, Edward L., 2016. "Statistical Inference for Partially Observed Markov Processes via the R Package pomp," Journal of Statistical Software, Foundation for Open Access Statistics, vol. 69(i12).
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