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CENcyclopedia: dynamic landscape of kinetochore architecture throughout the cell cycle

Author

Listed:
  • Yu-Chia Chen

    (University of Wisconsin-Madison
    University of Wisconsin-Madison)

  • Ece Kilic

    (University of Wisconsin-Madison)

  • Evelyn Wang

    (University of Wisconsin-Madison)

  • Will Rossman

    (University of Wisconsin-Madison)

  • Aussie Suzuki

    (University of Wisconsin-Madison
    University of Wisconsin-Madison
    University of Wisconsin-Madison)

Abstract

The kinetochore, an intricate macromolecular protein complex located on chromosomes, plays a pivotal role in orchestrating chromosome segregation. It functions as a versatile platform for microtubule assembly, diligently monitors microtubule binding fidelity, and acts as a force coupler. Comprising over 100 distinct proteins, many of which exist in multiple copies, the kinetochore’s composition dynamically changes throughout the cell cycle, responding to specific timing and conditions. This dynamicity is important for establishing functional kinetochores, yet the regulatory mechanisms of these dynamics have largely remained elusive. In this study, we employed advanced quantitative immunofluorescence techniques to meticulously chart the dynamics of kinetochore protein levels across the cell cycle. These findings offer a comprehensive view of the dynamic landscape of kinetochore architecture, shedding light on the detailed mechanisms of microtubule interaction and the nuanced characteristics of kinetochore proteins. This study significantly advances our understanding of the molecular coordination underlying chromosome segregation.

Suggested Citation

  • Yu-Chia Chen & Ece Kilic & Evelyn Wang & Will Rossman & Aussie Suzuki, 2025. "CENcyclopedia: dynamic landscape of kinetochore architecture throughout the cell cycle," Nature Communications, Nature, vol. 16(1), pages 1-24, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-62316-6
    DOI: 10.1038/s41467-025-62316-6
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    References listed on IDEAS

    as
    1. Aussie Suzuki & Benjamin L. Badger & Edward D. Salmon, 2015. "A quantitative description of Ndc80 complex linkage to human kinetochores," Nature Communications, Nature, vol. 6(1), pages 1-14, November.
    2. Yusuke Takenoshita & Masatoshi Hara & Tatsuo Fukagawa, 2022. "Recruitment of two Ndc80 complexes via the CENP-T pathway is sufficient for kinetochore functions," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    3. Lucie Y. Guo & Praveen Kumar Allu & Levani Zandarashvili & Kara L. McKinley & Nikolina Sekulic & Jennine M. Dawicki-McKenna & Daniele Fachinetti & Glennis A. Logsdon & Ryan M. Jamiolkowski & Don W. Cl, 2017. "Centromeres are maintained by fastening CENP-A to DNA and directing an arginine anchor-dependent nucleosome transition," Nature Communications, Nature, vol. 8(1), pages 1-15, August.
    4. Tatsuya Tsukahara & Yuji Tanno & Yoshinori Watanabe, 2010. "Phosphorylation of the CPC by Cdk1 promotes chromosome bi-orientation," Nature, Nature, vol. 467(7316), pages 719-723, October.
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    6. Elyse S. Fischer & Conny W. H. Yu & Johannes F. Hevler & Stephen H. McLaughlin & Sarah L. Maslen & Albert J. R. Heck & Stefan M. V. Freund & David Barford, 2022. "Juxtaposition of Bub1 and Cdc20 on phosphorylated Mad1 during catalytic mitotic checkpoint complex assembly," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
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