IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v16y2025i1d10.1038_s41467-025-61256-5.html
   My bibliography  Save this article

Regenerating aged bone marrow via a nitric oxide nanopump

Author

Listed:
  • Ke Li

    (Yangzhou University
    Affiliated Hospital of Yangzhou University
    The Key Laboratory of the Jiangsu Higher Education Institutions for Nucleic Acid & Cell Fate Regulation)

  • Sihan Hu

    (Yangzhou University
    The Key Laboratory of the Jiangsu Higher Education Institutions for Nucleic Acid & Cell Fate Regulation)

  • Hanwen Li

    (Affiliated Hospital of Yangzhou University
    The Key Laboratory of the Jiangsu Higher Education Institutions for Nucleic Acid & Cell Fate Regulation)

  • Wenzheng Lin

    (Yangzhou University
    Affiliated Hospital of Yangzhou University
    The Key Laboratory of the Jiangsu Higher Education Institutions for Nucleic Acid & Cell Fate Regulation)

  • Duoyi Zhao

    (Yangzhou University
    Affiliated Hospital of Yangzhou University
    The Key Laboratory of the Jiangsu Higher Education Institutions for Nucleic Acid & Cell Fate Regulation)

  • Zhuobin Xu

    (Yangzhou University
    The Key Laboratory of the Jiangsu Higher Education Institutions for Nucleic Acid & Cell Fate Regulation)

  • Chun Pan

    (Yangzhou University
    The Key Laboratory of the Jiangsu Higher Education Institutions for Nucleic Acid & Cell Fate Regulation)

  • Huihui Wang

    (Yangzhou University
    The Key Laboratory of the Jiangsu Higher Education Institutions for Nucleic Acid & Cell Fate Regulation)

  • Dandan Li

    (Yangzhou University
    The Key Laboratory of the Jiangsu Higher Education Institutions for Nucleic Acid & Cell Fate Regulation)

  • Jingjing Liu

    (Yangzhou University
    The Key Laboratory of the Jiangsu Higher Education Institutions for Nucleic Acid & Cell Fate Regulation)

  • Hao Chen

    (Yangzhou University
    Affiliated Hospital of Yangzhou University
    The Key Laboratory of the Jiangsu Higher Education Institutions for Nucleic Acid & Cell Fate Regulation)

Abstract

Resident leptin-receptor-expressing (LepR+) cells senescence in the aged bone marrow impairs the regenerative capacity of osteo- and other lineages of cells. In this study, a LepR+ cell-targeting nitric oxide (NO) nanopump with in vivo self-controlled turn-on ability was constructed to rejuvenate the LepR+ cells in the aged bone marrow. The nanopump co-entrapped hydrophobic chemiluminescence substrate and NO donor into the matrix of amphiphilic polymer through a nanoprecipitation process. The chemiluminescence substrate in the NO nanopump automatically reacts with the accumulated H2O2 in the aged bone marrow and then directly transfers the chemical energy to the NO donor to induce in situ NO release. The NO produced in situ within the aged bone marrow triggered the regeneration of the osteoblastic and the other niches in vivo through activating the glycolysis signaling in the senescent LepR+ cells. Conclusively, the constructed NO nanopump is a promising tool to counter aging-induced bone marrow disorders.

Suggested Citation

  • Ke Li & Sihan Hu & Hanwen Li & Wenzheng Lin & Duoyi Zhao & Zhuobin Xu & Chun Pan & Huihui Wang & Dandan Li & Jingjing Liu & Hao Chen, 2025. "Regenerating aged bone marrow via a nitric oxide nanopump," Nature Communications, Nature, vol. 16(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-61256-5
    DOI: 10.1038/s41467-025-61256-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-025-61256-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-025-61256-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Matthew J. Yousefzadeh & Rafael R. Flores & Yi Zhu & Zoe C. Schmiechen & Robert W. Brooks & Christy E. Trussoni & Yuxiang Cui & Luise Angelini & Kyoo-A Lee & Sara J. McGowan & Adam L. Burrack & Dong W, 2021. "An aged immune system drives senescence and ageing of solid organs," Nature, Nature, vol. 594(7861), pages 100-105, June.
    2. Jason B. Ross & Lara M. Myers & Joseph J. Noh & Madison M. Collins & Aaron B. Carmody & Ronald J. Messer & Erica Dhuey & Kim J. Hasenkrug & Irving L. Weissman, 2024. "Depleting myeloid-biased haematopoietic stem cells rejuvenates aged immunity," Nature, Nature, vol. 628(8006), pages 162-170, April.
    3. Lei Ding & Sean J. Morrison, 2013. "Haematopoietic stem cells and early lymphoid progenitors occupy distinct bone marrow niches," Nature, Nature, vol. 495(7440), pages 231-235, March.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Moujtaba Y. Kasmani & Paytsar Topchyan & Ashley K. Brown & Ryan J. Brown & Xiaopeng Wu & Yao Chen & Achia Khatun & Donia Alson & Yue Wu & Robert Burns & Chien-Wei Lin & Matthew R. Kudek & Jie Sun & We, 2023. "A spatial sequencing atlas of age-induced changes in the lung during influenza infection," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    2. Tiago C. Luis & Nikolaos Barkas & Joana Carrelha & Alice Giustacchini & Stefania Mazzi & Ruggiero Norfo & Bishan Wu & Affaf Aliouat & Jose A. Guerrero & Alba Rodriguez-Meira & Tiphaine Bouriez-Jones &, 2023. "Perivascular niche cells sense thrombocytopenia and activate hematopoietic stem cells in an IL-1 dependent manner," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    3. Taichi Nakatani & Tatsuki Sugiyama & Yoshiki Omatsu & Hitomi Watanabe & Gen Kondoh & Takashi Nagasawa, 2023. "Ebf3+ niche-derived CXCL12 is required for the localization and maintenance of hematopoietic stem cells," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    4. Eunmi Lee & Jason J. Hong & Gabriel Samcam Vargas & Natalie Sauerwald & Yong Wei & Xiang Hang & Chandra L. Theesfeld & Jean Arly A. Volmar & Jennifer M. Miller & Wei Wang & Sha Wang & Gary Laevsky & C, 2025. "CXCR4+ mammary gland macrophageal niche promotes tumor initiating cell activity and immune suppression during tumorigenesis," Nature Communications, Nature, vol. 16(1), pages 1-24, December.
    5. Runfeng Miao & Harim Chun & Xing Feng & Ana Cordeiro Gomes & Jungmin Choi & João P. Pereira, 2022. "Competition between hematopoietic stem and progenitor cells controls hematopoietic stem cell compartment size," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    6. Qiang Zhao & Young-Min Han & Ping Song & Zhixue Liu & Zuyi Yuan & Ming-Hui Zou, 2022. "Endothelial cell-specific expression of serine/threonine kinase 11 modulates dendritic cell differentiation," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    7. Stefano Suzzi & Tommaso Croese & Adi Ravid & Or Gold & Abbe R. Clark & Sedi Medina & Daniel Kitsberg & Miriam Adam & Katherine A. Vernon & Eva Kohnert & Inbar Shapira & Sergey Malitsky & Maxim Itkin &, 2023. "N-acetylneuraminic acid links immune exhaustion and accelerated memory deficit in diet-induced obese Alzheimer’s disease mouse model," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    8. Kaushik Bhattacharya & Samarpan Maiti & Szabolcs Zahoran & Lorenz Weidenauer & Dina Hany & Diana Wider & Lilia Bernasconi & Manfredo Quadroni & Martine Collart & Didier Picard, 2022. "Translational reprogramming in response to accumulating stressors ensures critical threshold levels of Hsp90 for mammalian life," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    9. Christina M. Termini & Amara Pang & Tiancheng Fang & Martina Roos & Vivian Y. Chang & Yurun Zhang & Nicollette J. Setiawan & Lia Signaevskaia & Michelle Li & Mindy M. Kim & Orel Tabibi & Paulina K. Li, 2021. "Neuropilin 1 regulates bone marrow vascular regeneration and hematopoietic reconstitution," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    10. Gülce Perçin & Konstantin Riege & Julia Fröbel & Jonas Metz & Stephan Culemann & Mathias Lesche & Susanne Reinhardt & Thomas Höfer & Steve Hoffmann & Claudia Waskow, 2025. "Embryonic macrophages orchestrate niche cell homeostasis for the establishment of the definitive hematopoietic stem cell pool," Nature Communications, Nature, vol. 16(1), pages 1-14, December.
    11. Xu Feng & Liwen Wang & Ruoyu Zhou & Rui Zhou & Linyun Chen & Hui Peng & Yan Huang & Qi Guo & Xianghang Luo & Haiyan Zhou, 2023. "Senescent immune cells accumulation promotes brown adipose tissue dysfunction during aging," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    12. Yonggang Fan & Weixin Zhang & Xiusheng Huang & Mingzhe Fan & Chenhao Shi & Lantian Zhao & Guofu Pi & Huafeng Zhang & Shuangfei Ni, 2024. "Senescent-like macrophages mediate angiogenesis for endplate sclerosis via IL-10 secretion in male mice," Nature Communications, Nature, vol. 15(1), pages 1-21, December.
    13. Anna Konturek-Ciesla & Qinyu Zhang & Shabnam Kharazi & David Bryder, 2025. "A non-genotoxic stem cell therapy boosts lymphopoiesis and averts age-related blood diseases in mice," Nature Communications, Nature, vol. 16(1), pages 1-13, December.
    14. Xiaoduo Tang & Jingyan Ren & Xin Wei & Tao Wang & Haiqiu Li & Yihan Sun & Yang Liu & Mingli Chi & Shoujun Zhu & Laijin Lu & Junhu Zhang & Bai Yang, 2023. "Exploiting synergistic effect of CO/NO gases for soft tissue transplantation using a hydrogel patch," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    15. Nini Zhang & Xinpei Wang & Mengya Feng & Min Li & Jing Wang & Hongyan Yang & Siyu He & Ziqi Xia & Lei Shang & Xun Jiang & Mao Sun & Yuanming Wu & Chaoxue Ren & Xing Zhang & Jia Li & Feng Gao, 2024. "Early-life exercise induces immunometabolic epigenetic modification enhancing anti-inflammatory immunity in middle-aged male mice," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    16. Madison L. Doolittle & Dominik Saul & Japneet Kaur & Jennifer L. Rowsey & Stephanie J. Vos & Kevin D. Pavelko & Joshua N. Farr & David G. Monroe & Sundeep Khosla, 2023. "Multiparametric senescent cell phenotyping reveals targets of senolytic therapy in the aged murine skeleton," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    17. Adrienne Anginot & Julie Nguyen & Zeina Abou Nader & Vincent Rondeau & Amélie Bonaud & Maria Kalogeraki & Antoine Boutin & Julia P. Lemos & Valeria Bisio & Joyce Koenen & Lea Hanna Doumit Sakr & Amand, 2023. "WHIM Syndrome-linked CXCR4 mutations drive osteoporosis," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    18. Betz, Ulrich A.K. & Arora, Loukik & Assal, Reem A. & Azevedo, Hatylas & Baldwin, Jeremy & Becker, Michael S. & Bostock, Stefan & Cheng, Vinton & Egle, Tobias & Ferrari, Nicola & Schneider-Futschik, El, 2023. "Game changers in science and technology - now and beyond," Technological Forecasting and Social Change, Elsevier, vol. 193(C).
    19. Xu Zhang & Vesselina M. Pearsall & Chase M. Carver & Elizabeth J. Atkinson & Benjamin D. S. Clarkson & Ethan M. Grund & Michelle Baez-Faria & Kevin D. Pavelko & Jennifer M. Kachergus & Thomas A. White, 2022. "Rejuvenation of the aged brain immune cell landscape in mice through p16-positive senescent cell clearance," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    20. Carmen Stecher & Romana Bischl & Anna Schmid-Böse & Stefanie Ferstl & Elisabeth Potzmann & Magdalena Frank & Nina Braun & Matthias Farlik & Richard A. Flavell & Dietmar Herndler-Brandstetter, 2025. "Heterogeneity of IL-15-expressing mesenchymal stromal cells controls natural killer cell development and immune cell homeostasis," Nature Communications, Nature, vol. 16(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-61256-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.