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Analysis of the Rehmannia chingii geneome identifies RcCYP72H7 as an epoxidase in iridoid glycoside biosynthesis

Author

Listed:
  • Fengqing Wang

    (Henan Agricultural University)

  • Zhennan Jiang

    (China Academy of Chinese Medical Sciences (CACMS))

  • Junge Gao

    (Henan Agricultural University)

  • Chunyan Miao

    (Henan Agricultural University)

  • Ci Song

    (Henan Agricultural University)

  • Yahe Yang

    (Henan Agricultural University)

  • Ning Ding

    (Henan Agricultural University)

  • Yajing Li

    (Shanghai University of Traditional Chinese Medicine)

  • Hongzheng Sun

    (Henan Agricultural University)

  • Caixia Xie

    (Henan University of Chinese Medicine)

  • Bao Zhang

    (Henan University of Chinese Medicine)

  • Pengyu Zhang

    (Henan Agricultural University)

  • Li Gu

    (Fujian Agriculture and Forestry University)

  • Qiuwen Hao

    (Henan Agricultural University)

  • Zhongyi Zhang

    (Fujian Agriculture and Forestry University)

  • Luqi Huang

    (China Academy of Chinese Medical Sciences (CACMS))

  • Yuan Yuan

    (China Academy of Chinese Medical Sciences (CACMS))

Abstract

Rehmannia chingii (2n = 2x = 28) is an important folk medicinal plant with high therapeutic value, particularly due to its richness in iridoid glycosides. However, research on its evolution and gene functional identification has been hindered by the lack of a high-quality genome. Here, we present the 1.169 Gb telomere-to-telomere (T2T) genome sequence of R. chingii. Phylogenetic analysis confirms that Rehmannia belongs to the Orobanchaceae family. We find that structural genes of the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway and the iridoid pathway are predominantly expressed in R. chingii leaves. Further analyses reveal a cytochrome P450 gene cluster localized on chromosome 8, and identify RcCYP72H7 within this cluster as an aucubin epoxidase, capable of catalyzing aucubin epoxidation to form catalpol. The genome offers valuable resources for studying iridoid glycoside biosynthesis and the evolutionary history of Rehmannia, and will help to faciliate genetic improvement of R. chingii for pharmaceutical and health-related applications.

Suggested Citation

  • Fengqing Wang & Zhennan Jiang & Junge Gao & Chunyan Miao & Ci Song & Yahe Yang & Ning Ding & Yajing Li & Hongzheng Sun & Caixia Xie & Bao Zhang & Pengyu Zhang & Li Gu & Qiuwen Hao & Zhongyi Zhang & Lu, 2025. "Analysis of the Rehmannia chingii geneome identifies RcCYP72H7 as an epoxidase in iridoid glycoside biosynthesis," Nature Communications, Nature, vol. 16(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-60909-9
    DOI: 10.1038/s41467-025-60909-9
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    2. Yuannian Jiao & Norman J. Wickett & Saravanaraj Ayyampalayam & André S. Chanderbali & Lena Landherr & Paula E. Ralph & Lynn P. Tomsho & Yi Hu & Haiying Liang & Pamela S. Soltis & Douglas E. Soltis & S, 2011. "Ancestral polyploidy in seed plants and angiosperms," Nature, Nature, vol. 473(7345), pages 97-100, May.
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