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Wastewater surveillance of SARS-CoV-2 from aircraft to citywide monitoring

Author

Listed:
  • Mariel Perez-Zabaleta

    (AlbaNova University Center
    Research Group for Food Microbiology and Hygiene, National Food Institute, Technical University of Denmark)

  • Carlo Berg

    (Public Health Agency of Sweden)

  • Neus Latorre-Margalef

    (Public Health Agency of Sweden)

  • Isaac Owusu-Agyeman

    (AlbaNova University Center)

  • Ayda Kiyar

    (AlbaNova University Center)

  • Helene Botnen

    (Public Health Agency of Sweden)

  • Caroline Schönning

    (Public Health Agency of Sweden)

  • Luisa W. Hugerth

    (Uppsala University)

  • Zeynep Cetecioglu

    (AlbaNova University Center)

Abstract

Wastewater monitoring is highly efficient in SARS-CoV-2 surveillance for tracking virus spread through travel, surpassing traditional airport passenger testing. This study explored the links between SARS-CoV-2 contents and variants from aircraft to city, assessing the impact of detected variants from international travellers versus the local population. A total of 969 variants using next-generation sequencing (NGS) were examined to understand the links between—aircraft, Arlanda airport, wastewater treatment plants (WWTPs), and Stockholm city—and compared these to variants detected in Stockholm hospitals from January to May 2023. SARS-CoV-2 contents in WWTPs reflected local infection rates, requiring analysis from multiple plants for an accurate city-wide infection assessment. Variants initially detected in aircraft arriving from China did not spread widely during the study period. RT-qPCR is adequate for the detection of specific variants in wastewater, including Variants Under Monitoring. However, NGS remains a powerful method for identifying novel variants. Wastewater monitoring was more effective than clinical testing in the early detection of specific variants, with notable delays observed in clinical surveillance. Furthermore, a broad range of variants are detected in wastewater that surpasses clinical tests. This underscores the vital role of wastewater-based epidemiology in managing future outbreaks and enhancing global health security.

Suggested Citation

  • Mariel Perez-Zabaleta & Carlo Berg & Neus Latorre-Margalef & Isaac Owusu-Agyeman & Ayda Kiyar & Helene Botnen & Caroline Schönning & Luisa W. Hugerth & Zeynep Cetecioglu, 2025. "Wastewater surveillance of SARS-CoV-2 from aircraft to citywide monitoring," Nature Communications, Nature, vol. 16(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-60490-1
    DOI: 10.1038/s41467-025-60490-1
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    References listed on IDEAS

    as
    1. Juan Li & Shengjie Lai & George F. Gao & Weifeng Shi, 2021. "The emergence, genomic diversity and global spread of SARS-CoV-2," Nature, Nature, vol. 600(7889), pages 408-418, December.
    2. Smruthi Karthikeyan & Joshua I. Levy & Peter Hoff & Greg Humphrey & Amanda Birmingham & Kristen Jepsen & Sawyer Farmer & Helena M. Tubb & Tommy Valles & Caitlin E. Tribelhorn & Rebecca Tsai & Stefan A, 2022. "Wastewater sequencing reveals early cryptic SARS-CoV-2 variant transmission," Nature, Nature, vol. 609(7925), pages 101-108, September.
    3. Nakamura, Hiroki & Managi, Shunsuke, 2020. "Airport risk of importation and exportation of the COVID-19 pandemic," Transport Policy, Elsevier, vol. 96(C), pages 40-47.
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