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Septin higher-order structure on yeast membranes in vitro

Author

Listed:
  • James A. Goodchild

    (Department of Anesthesiology)

  • Brandy N. Curtis

    (Department of Biochemistry and Biophysics
    Department of Cell Biology)

  • Yangang Pan

    (Department of Anesthesiology)

  • Yining Jiang

    (Department of Anesthesiology)

  • Fang Jiao

    (Chinese Academy of Sciences)

  • Amy S. Gladfelter

    (Department of Cell Biology)

  • Simon Scheuring

    (Department of Anesthesiology
    Department of Physiology and Biophysics)

Abstract

Septins are cytoskeletal proteins that form filaments and higher-order structures, and remodel membranes in a variety of processes. Structural and cell biological studies provided atomic- and micro-scale details, but the understanding of septin assembly at the mesoscale is limited. Here, we used high-speed atomic force microscopy (HS-AFM) to analyze yeast septin assembly on yeast supported lipid bilayers (SLBs). We found the coexistence of three lipid phases in yeast membranes, where septin polymerized selectively on the liquid-disordered phase. Septin filaments adhered to membranes with a conserved face; and paired filaments, previously reported in less native environments, were not observed. Additionally, septin filaments exhibited lateral and longitudinal alignment. We used HS-AFM force-sweep experiments to disrupt septin structures and observe organizational recovery through self-templating. Finally, septin filaments stacked, where higher layer filament alignment was templated by the layer below. Thus, septins encode their 3D-structural organization, likely tunable by the membrane and bulk environment.

Suggested Citation

  • James A. Goodchild & Brandy N. Curtis & Yangang Pan & Yining Jiang & Fang Jiao & Amy S. Gladfelter & Simon Scheuring, 2025. "Septin higher-order structure on yeast membranes in vitro," Nature Communications, Nature, vol. 16(1), pages 1-11, December.
  • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-60344-w
    DOI: 10.1038/s41467-025-60344-w
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    References listed on IDEAS

    as
    1. Katy Ong & Carsten Wloka & Satoshi Okada & Tatyana Svitkina & Erfei Bi, 2014. "Architecture and dynamic remodelling of the septin cytoskeleton during the cell cycle," Nature Communications, Nature, vol. 5(1), pages 1-10, December.
    2. Stefano Vanni & Hisaaki Hirose & Hélène Barelli & Bruno Antonny & Romain Gautier, 2014. "A sub-nanometre view of how membrane curvature and composition modulate lipid packing and protein recruitment," Nature Communications, Nature, vol. 5(1), pages 1-10, December.
    3. Alexandre Beber & Cyntia Taveneau & Manuela Nania & Feng-Ching Tsai & Aurelie Cicco & Patricia Bassereau & Daniel Lévy & João T. Cabral & Hervé Isambert & Stéphanie Mangenot & Aurélie Bertin, 2019. "Membrane reshaping by micrometric curvature sensitive septin filaments," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    4. Nebojsa Jukic & Alma P. Perrino & Frédéric Humbert & Aurélien Roux & Simon Scheuring, 2022. "Snf7 spirals sense and alter membrane curvature," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    5. Fang Jiao & Kevin S. Cannon & Yi-Chih Lin & Amy S. Gladfelter & Simon Scheuring, 2020. "The hierarchical assembly of septins revealed by high-speed AFM," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
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    8. Alma P. Perrino & Atsushi Miyagi & Simon Scheuring, 2021. "Single molecule kinetics of bacteriorhodopsin by HS-AFM," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
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