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A continental scale analysis reveals widespread root bimodality

Author

Listed:
  • Mingzhen Lu

    (Santa Fe Institute
    Stanford University
    New York University)

  • Sili Wang

    (Columbia University)

  • Avni Malhotra

    (Stanford University
    University of Zurich
    Pacific Northwest National Laboratory)

  • Shersingh Joseph Tumber-Dávila

    (Stanford University
    Harvard University
    Dartmouth College)

  • Samantha Weintraub-Leff

    (Battelle)

  • M. Luke McCormack

    (The Morton Arboretum)

  • Xingchen Tony Wang

    (Boston College)

  • Robert B. Jackson

    (Stanford University
    Stanford University)

Abstract

An improved understanding of root vertical distribution is crucial for assessing plant-soil-atmosphere interactions and their influence on the land carbon sink. Here, we analyze a continental-scale dataset of fine roots reaching 2 meters depth, spanning from Alaskan tundra to Puerto Rican forests. Contrary to the expectation that fine root abundance decays exponentially with depth, we found root bimodality at ~20% of 44 sites, with secondary biomass peaks often below 1 m. Root bimodality was more likely in areas with low total fine root biomass and was more frequent in shrublands than grasslands. Notably, secondary peaks coincided with high soil nitrogen content at depth. Our analyses suggest that deep soil nutrients tend to be underexploited, while root bimodality offers plants a mechanism to tap into deep soil resources. Our findings add to the growing recognition that deep soil dynamics are systematically overlooked, and calls for more research attention to this deep frontier in the face of global environmental change.

Suggested Citation

  • Mingzhen Lu & Sili Wang & Avni Malhotra & Shersingh Joseph Tumber-Dávila & Samantha Weintraub-Leff & M. Luke McCormack & Xingchen Tony Wang & Robert B. Jackson, 2025. "A continental scale analysis reveals widespread root bimodality," Nature Communications, Nature, vol. 16(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-60055-2
    DOI: 10.1038/s41467-025-60055-2
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    as
    1. Wannes Hubau & Simon L. Lewis & Oliver L. Phillips & Kofi Affum-Baffoe & Hans Beeckman & Aida Cuní-Sanchez & Armandu K. Daniels & Corneille E. N. Ewango & Sophie Fauset & Jacques M. Mukinzi & Douglas , 2020. "Asynchronous carbon sink saturation in African and Amazonian tropical forests," Nature, Nature, vol. 579(7797), pages 80-87, March.
    2. McCormack, M. Luke & Crisfield, Elizabeth & Raczka, Brett & Schnekenburger, Frank & Eissenstat, David M. & Smithwick, Erica A.H., 2015. "Sensitivity of four ecological models to adjustments in fine root turnover rate," Ecological Modelling, Elsevier, vol. 297(C), pages 107-117.
    3. Richard D. Bardgett & Wim H. van der Putten, 2014. "Belowground biodiversity and ecosystem functioning," Nature, Nature, vol. 515(7528), pages 505-511, November.
    4. Jérôme Balesdent & Isabelle Basile-Doelsch & Joël Chadoeuf & Sophie Cornu & Delphine Derrien & Zuzana Fekiacova & Christine Hatté, 2018. "Atmosphere–soil carbon transfer as a function of soil depth," Nature, Nature, vol. 559(7715), pages 599-602, July.
    5. Peter B. Reich & Sarah E. Hobbie & Tali Lee & David S. Ellsworth & Jason B. West & David Tilman & Johannes M. H. Knops & Shahid Naeem & Jared Trost, 2006. "Nitrogen limitation constrains sustainability of ecosystem response to CO2," Nature, Nature, vol. 440(7086), pages 922-925, April.
    6. Dan Kou & Guibiao Yang & Fei Li & Xuehui Feng & Dianye Zhang & Chao Mao & Qiwen Zhang & Yunfeng Peng & Chengjun Ji & Qiuan Zhu & Yunting Fang & Xueyan Liu & Xu-Ri & Siqi Li & Jia Deng & Xunhua Zheng &, 2020. "Progressive nitrogen limitation across the Tibetan alpine permafrost region," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    7. Zeqing Ma & Dali Guo & Xingliang Xu & Mingzhen Lu & Richard D. Bardgett & David M. Eissenstat & M. Luke McCormack & Lars O. Hedin, 2018. "Erratum: Evolutionary history resolves global organization of root functional traits," Nature, Nature, vol. 556(7699), pages 135-135, April.
    8. Zeqing Ma & Dali Guo & Xingliang Xu & Mingzhen Lu & Richard D. Bardgett & David M. Eissenstat & M. Luke McCormack & Lars O. Hedin, 2018. "Evolutionary history resolves global organization of root functional traits," Nature, Nature, vol. 555(7694), pages 94-97, March.
    9. Gonzalo Miguez-Macho & Ying Fan, 2021. "Spatiotemporal origin of soil water taken up by vegetation," Nature, Nature, vol. 598(7882), pages 624-628, October.
    10. Deliang Kong & Junjian Wang & Huifang Wu & Oscar J. Valverde-Barrantes & Ruili Wang & Hui Zeng & Paul Kardol & Haiyan Zhang & Yulong Feng, 2019. "Nonlinearity of root trait relationships and the root economics spectrum," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    11. Jose Ameijeiras-Alonso & Rosa M. Crujeiras & Alberto Rodríguez-Casal, 2019. "Mode testing, critical bandwidth and excess mass," TEST: An Official Journal of the Spanish Society of Statistics and Operations Research, Springer;Sociedad de Estadística e Investigación Operativa, vol. 28(3), pages 900-919, September.
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