IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v16y2025i1d10.1038_s41467-025-59026-4.html
   My bibliography  Save this article

Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication

Author

Listed:
  • Byungjun Kang

    (Yonsei University)

  • Eunseon Jeong

    (Yonsei University)

  • Seung Yeop Han

    (Yonsei University
    Yonsei University)

  • Jeong Hyun Heo

    (Yonsei University College of Medicine)

  • Yunam Lee

    (Yonsei University)

  • Suah Choi

    (Yonsei University)

  • Yunjung Choi

    (Yonsei University)

  • Donyoung Kang

    (Yonsei University)

  • Youn-Hoo Hwang

    (Yonsei University)

  • Jiin Lee

    (Yonsei University)

  • Jung Hwa Seo

    (Yonsei University College of Medicine)

  • Jinyoung Kim

    (Yonsei University College of Medicine
    Yonsei University College of Medicine)

  • Inhea Jeong

    (Yonsei University)

  • Enji Kim

    (Yonsei University
    Institute for Basic Science (IBS))

  • Juyoung Lee

    (Yonsei University)

  • Dae-Eun Kim

    (Yonsei University)

  • Jang-Ung Park

    (Yonsei University
    Institute for Basic Science (IBS)
    Yonsei University College of Medicine
    Yonsei University)

  • Sung-Rae Cho

    (Yonsei University College of Medicine
    Yonsei University College of Medicine
    Yonsei University College of Medicine
    Yonsei University College of Medicine)

  • Yoonhee Jin

    (Yonsei University College of Medicine)

  • Seung-Woo Cho

    (Yonsei University
    Institute for Basic Science (IBS)
    Yonsei University)

  • Hyungsuk Lee

    (Yonsei University)

Abstract

To build in vitro tissues for therapeutic applications, it is essential to replicate the spatial distribution of cells that occurs during morphogenesis in vivo. However, it remains technically challenging to simultaneously regulate the geometric alignment and aggregation of cells during tissue fabrication. Here, we introduce the acoustofluidic bioassembly induced morphogenesis, which is the combination of precise arrangement of cells by the mechanical forces produced by acoustofluidic cues, and the morphological and functional changes of cells in the following in vitro and in vivo cultures. The acoustofluidic bioassembly can be used to create tissues with regulated nano-, micro-, and macro-structures. We demonstrate that the neuromuscular tissue fabricated with the acoustofluidic bioassembly exhibits enhanced contraction dynamics, electrophysiology, and therapeutic efficacy. The potential of the acoustofluidic bioassembly as an in situ application is demonstrated by fabricating artificial tissues at the defect sites of living tissues. The acoustofluidic bioassembly induced morphogenesis can provide a pioneering platform to fabricate tissues for biomedical applications.

Suggested Citation

  • Byungjun Kang & Eunseon Jeong & Seung Yeop Han & Jeong Hyun Heo & Yunam Lee & Suah Choi & Yunjung Choi & Donyoung Kang & Youn-Hoo Hwang & Jiin Lee & Jung Hwa Seo & Jinyoung Kim & Inhea Jeong & Enji Ki, 2025. "Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication," Nature Communications, Nature, vol. 16(1), pages 1-19, December.
  • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-59026-4
    DOI: 10.1038/s41467-025-59026-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-025-59026-4
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-025-59026-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Marco Quarta & Melinda Cromie & Robert Chacon & Justin Blonigan & Victor Garcia & Igor Akimenko & Mark Hamer & Patrick Paine & Merel Stok & Joseph B. Shrager & Thomas A. Rando, 2017. "Bioengineered constructs combined with exercise enhance stem cell-mediated treatment of volumetric muscle loss," Nature Communications, Nature, vol. 8(1), pages 1-17, August.
    2. David J. Collins & Belinda Morahan & Jose Garcia-Bustos & Christian Doerig & Magdalena Plebanski & Adrian Neild, 2015. "Two-dimensional single-cell patterning with one cell per well driven by surface acoustic waves," Nature Communications, Nature, vol. 6(1), pages 1-11, December.
    3. Jeiwan Tan & Byungjun Kang & Kyungmin Kim & Donyoung Kang & Hyungsoo Lee & Sunihl Ma & Gyumin Jang & Hyungsuk Lee & Jooho Moon, 2022. "Hydrogel protection strategy to stabilize water-splitting photoelectrodes," Nature Energy, Nature, vol. 7(6), pages 537-547, June.
    4. Kai Melde & Andrew G. Mark & Tian Qiu & Peer Fischer, 2016. "Holograms for acoustics," Nature, Nature, vol. 537(7621), pages 518-522, September.
    5. Lingjun Rao & Ying Qian & Alastair Khodabukus & Thomas Ribar & Nenad Bursac, 2018. "Engineering human pluripotent stem cells into a functional skeletal muscle tissue," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    6. Michael Baudoin & Jean-Louis Thomas & Roudy Al Sahely & Jean-Claude Gerbedoen & Zhixiong Gong & Aude Sivery & Olivier Bou Matar & Nikolay Smagin & Peter Favreau & Alexis Vlandas, 2020. "Spatially selective manipulation of cells with single-beam acoustical tweezers," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    7. Byungjun Kang & Jeiwan Tan & Kyungmin Kim & Donyoung Kang & Hyungsoo Lee & Sunihl Ma & Young Sun Park & Juwon Yun & Soobin Lee & Chan Uk Lee & Gyumin Jang & Jeongyoub Lee & Jooho Moon & Hyungsuk Lee, 2024. "Stable water splitting using photoelectrodes with a cryogelated overlayer," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    8. Byungjun Kang & Jisoo Shin & Hyun-Ji Park & Chanryeol Rhyou & Donyoung Kang & Shin-Jeong Lee & Young-sup Yoon & Seung-Woo Cho & Hyungsuk Lee, 2018. "High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Ruoqin Zhang & Xichuan Zhao & Jinzhi Li & Di Zhou & Honglian Guo & Zhi-yuan Li & Feng Li, 2024. "Programmable photoacoustic patterning of microparticles in air," Nature Communications, Nature, vol. 15(1), pages 1-8, December.
    2. Liang Shen & Zhenhua Tian & Kaichun Yang & Joseph Rich & Jianping Xia & Neil Upreti & Jinxin Zhang & Chuyi Chen & Nanjing Hao & Zhichao Pei & Tony Jun Huang, 2024. "Joint subarray acoustic tweezers enable controllable cell translation, rotation, and deformation," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    3. Jan Durrer & Prajwal Agrawal & Ali Ozgul & Stephan C. F. Neuhauss & Nitesh Nama & Daniel Ahmed, 2022. "A robot-assisted acoustofluidic end effector," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Joseph Rufo & Peiran Zhang & Ruoyu Zhong & Luke P. Lee & Tony Jun Huang, 2022. "A sound approach to advancing healthcare systems: the future of biomedical acoustics," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    5. Zengxia Pei & Hao Tan & Jinxing Gu & Linguo Lu & Xin Zeng & Tianqi Zhang & Cheng Wang & Luyao Ding & Patrick J. Cullen & Zhongfang Chen & Shenlong Zhao, 2023. "A polymeric hydrogel electrocatalyst for direct water oxidation," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    6. Yurou Jia & Suying Zhang & Xuan Zhang & Houyou Long & Caibin Xu & Yechao Bai & Ying Cheng & Dajian Wu & Mingxi Deng & Cheng-Wei Qiu & Xiaojun Liu, 2024. "Compact meta-differentiator for achieving isotropically high-contrast ultrasonic imaging," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    7. Matthew Stein & Sam Keller & Yujie Luo & Ognjen Ilic, 2022. "Shaping contactless radiation forces through anomalous acoustic scattering," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    8. Gazendra Shakya & Tao Yang & Yu Gao & Apresio K. Fajrial & Baowen Li & Massimo Ruzzene & Mark A. Borden & Xiaoyun Ding, 2022. "Acoustically manipulating internal structure of disk-in-sphere endoskeletal droplets," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    9. Mahdi Derayatifar & Mohsen Habibi & Rama Bhat & Muthukumaran Packirisamy, 2024. "Holographic direct sound printing," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    10. Sudhagar Pitchaimuthu & Kishore Sridharan & Sanjay Nagarajan & Sengeni Ananthraj & Peter Robertson & Moritz F. Kuehnel & Ángel Irabien & Mercedes Maroto-Valer, 2022. "Solar Hydrogen Fuel Generation from Wastewater—Beyond Photoelectrochemical Water Splitting: A Perspective," Energies, MDPI, vol. 15(19), pages 1-23, October.
    11. Li Zhang & Xiaoju Yang & Qing Yuan & Zhiming Wei & Jie Ding & Tianshu Chu & Chao Rong & Qiao Zhang & Zhenkun Ye & Fu-Zhen Xuan & Yueming Zhai & Bowei Zhang & Xuan Yang, 2023. "Elucidating the structure-stability relationship of Cu single-atom catalysts using operando surface-enhanced infrared absorption spectroscopy," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    12. Zhiyuan Zhang & Alexander Sukhov & Jens Harting & Paolo Malgaretti & Daniel Ahmed, 2022. "Rolling microswarms along acoustic virtual walls," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    13. Qinghao Mao & Brady Wu & Bryan VanSaders & Heinrich M. Jaeger, 2025. "Structural reconfiguration of interacting multi-particle systems through parametric pumping," Nature Communications, Nature, vol. 16(1), pages 1-9, December.
    14. Wan Jae Dong & Jan Paul Menzel & Kejian Li & Zhengwei Ye & Zhuoran Long & Ishtiaque Ahmed Navid & Ke R. Yang & Yixin Xiao & Victor S. Batista & Zetian Mi, 2025. "Nitrate reduction to ammonia catalyzed by GaN/Si photoelectrodes with metal clusters," Nature Communications, Nature, vol. 16(1), pages 1-11, December.
    15. Mittal, Prateek & Christopoulos, Giorgos & Subramanian, Sriram, 2024. "Energy enhancement through noise minimization using acoustic metamaterials in a wind farm," Renewable Energy, Elsevier, vol. 224(C).
    16. Yucheng Luo & Qiu Yin & Keke Chen & Zhaoyu Deng & Xiaozhou Liu & Yinning Zhou & Benpeng Zhu & Wenming Zhang & Zhichao Ma, 2025. "Superselective embolic particle guidance in vessel networks via shape-adaptive acoustic manipulation," Nature Communications, Nature, vol. 16(1), pages 1-12, December.
    17. Alessia Urzi & Ines Lahmann & Lan Vi N. Nguyen & Benjamin R. Rost & Angélica García-Pérez & Noemie Lelievre & Megan E. Merritt-Garza & Han C. Phan & Gary J. Bassell & Wilfried Rossoll & Sebastian Diec, 2023. "Efficient generation of a self-organizing neuromuscular junction model from human pluripotent stem cells," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    18. Zhonghui Zhu & Matyas Daboczi & Minzhi Chen & Yimin Xuan & Xianglei Liu & Salvador Eslava, 2024. "Ultrastable halide perovskite CsPbBr3 photoanodes achieved with electrocatalytic glassy-carbon and boron-doped diamond sheets," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    19. Byungjun Kang & Jeiwan Tan & Kyungmin Kim & Donyoung Kang & Hyungsoo Lee & Sunihl Ma & Young Sun Park & Juwon Yun & Soobin Lee & Chan Uk Lee & Gyumin Jang & Jeongyoub Lee & Jooho Moon & Hyungsuk Lee, 2024. "Stable water splitting using photoelectrodes with a cryogelated overlayer," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    20. Alexia Campo Fonseca & Chaim Glück & Jeanne Droux & Yann Ferry & Carole Frei & Susanne Wegener & Bruno Weber & Mohamad El Amki & Daniel Ahmed, 2023. "Ultrasound trapping and navigation of microrobots in the mouse brain vasculature," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-59026-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.