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Dscamb regulates cone mosaic formation in zebrafish via filopodium-mediated homotypic recognition

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  • Dongpeng Hu

    (Okinawa Institute of Science and Technology Graduate University)

  • Ichiro Masai

    (Okinawa Institute of Science and Technology Graduate University)

Abstract

Cone photoreceptors assemble to form a regular mosaic pattern in vertebrate retinas. In zebrafish, four distinct spectral cone types (red, green, blue, and ultraviolet), form a lattice-like pattern. However, the mechanism of cone mosaic formation has been unknown. Here we show that Down Syndrome Cell Adhesion Molecule b (Dscamb) regulates the cone mosaic pattern in zebrafish, especially via red-cone spacing. During photoreceptor differentiation, newly formed cones extend filopodium-like processes laterally to apical surfaces of neighboring cones. Interestingly, red cones extend filopodia, but promptly retract them when they meet their own cone type, suggesting filopodium-mediated, homotypic recognition and self-avoidance. This self-avoidance is compromised in zebrafish dscamb mutants, leading to abnormal clustering of red cones and subsequent disruption of regular cone spacing. Thus, apical filopodium-mediated spacing of the same cone type depends on Dscamb and is essential for cone mosaic formation in zebrafish.

Suggested Citation

  • Dongpeng Hu & Ichiro Masai, 2025. "Dscamb regulates cone mosaic formation in zebrafish via filopodium-mediated homotypic recognition," Nature Communications, Nature, vol. 16(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-57506-1
    DOI: 10.1038/s41467-025-57506-1
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    References listed on IDEAS

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    1. Chuyan Liu & Olena Trush & Xujun Han & Miaoxing Wang & Rie Takayama & Tetsuo Yasugi & Takashi Hayashi & Makoto Sato, 2020. "Dscam1 establishes the columnar units through lineage-dependent repulsion between sister neurons in the fly brain," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    2. Jeremy N. Kay & Monica W. Chu & Joshua R. Sanes, 2012. "MEGF10 and MEGF11 mediate homotypic interactions required for mosaic spacing of retinal neurons," Nature, Nature, vol. 483(7390), pages 465-469, March.
    3. S. Sean Millard & John J. Flanagan & Kartik S. Pappu & Wei Wu & S. Lawrence Zipursky, 2007. "Dscam2 mediates axonal tiling in the Drosophila visual system," Nature, Nature, vol. 447(7145), pages 720-724, June.
    4. Daisuke Hattori & Ebru Demir & Ho Won Kim & Erika Viragh & S. Lawrence Zipursky & Barry J. Dickson, 2007. "Dscam diversity is essential for neuronal wiring and self-recognition," Nature, Nature, vol. 449(7159), pages 223-227, September.
    5. Peter G. Fuerst & Amane Koizumi & Richard H. Masland & Robert W. Burgess, 2008. "Neurite arborization and mosaic spacing in the mouse retina require DSCAM," Nature, Nature, vol. 451(7177), pages 470-474, January.
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