IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-49683-2.html

IDEAS is struggling with massive bot traffic, please be patient. We apologize for the inconvenience.

   My bibliography  Save this article

Strain dynamics of contaminating bacteria modulate the yield of ethanol biorefineries

Author

Listed:
  • Felipe Senne Oliveira Lino

    (Technical University of Denmark)

  • Shilpa Garg

    (Technical University of Denmark)

  • Simone S. Li

    (Technical University of Denmark
    University of Queensland)

  • Maria-Anna Misiakou

    (Technical University of Denmark)

  • Kang Kang

    (Leibniz Institute for Natural Product Research and Infection Biology)

  • Bruno Labate Vale da Costa

    (Escola de Engenharia de Alimentos da Universidade de Campinas)

  • Tobias Svend-Aage Beyer-Pedersen

    (Technical University of Denmark)

  • Thamiris Guerra Giacon

    (Departamento de Engenharia Química da Escola Politécnica da Universidade de São Paulo. Universidade de São Paulo)

  • Thiago Olitta Basso

    (Departamento de Engenharia Química da Escola Politécnica da Universidade de São Paulo. Universidade de São Paulo)

  • Gianni Panagiotou

    (Leibniz Institute for Natural Product Research and Infection Biology)

  • Morten Otto Alexander Sommer

    (Technical University of Denmark)

Abstract

Bioethanol is a sustainable energy alternative and can contribute to global greenhouse-gas emission reductions by over 60%. Its industrial production faces various bottlenecks, including sub-optimal efficiency resulting from bacteria. Broad-spectrum removal of these contaminants results in negligible gains, suggesting that the process is shaped by ecological interactions within the microbial community. Here, we survey the microbiome across all process steps at two biorefineries, over three timepoints in a production season. Leveraging shotgun metagenomics and cultivation-based approaches, we identify beneficial bacteria and find improved outcome when yeast-to-bacteria ratios increase during fermentation. We provide a microbial gene catalogue which reveals bacteria-specific pathways associated with performance. We also show that Limosilactobacillus fermentum overgrowth lowers production, with one strain reducing yield by ~5% in laboratory fermentations, potentially due to its metabolite profile. Temperature is found to be a major driver for strain-level dynamics. Improved microbial management strategies could unlock environmental and economic gains in this US $ 60 billion industry enabling its wider adoption.

Suggested Citation

  • Felipe Senne Oliveira Lino & Shilpa Garg & Simone S. Li & Maria-Anna Misiakou & Kang Kang & Bruno Labate Vale da Costa & Tobias Svend-Aage Beyer-Pedersen & Thamiris Guerra Giacon & Thiago Olitta Basso, 2024. "Strain dynamics of contaminating bacteria modulate the yield of ethanol biorefineries," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49683-2
    DOI: 10.1038/s41467-024-49683-2
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-49683-2
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-49683-2?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Felipe Lino & Djordje Bajic & Jean Celestin Charles Vila & Alvaro Sánchez & Morten Otto Alexander Sommer, 2021. "Complex yeast–bacteria interactions affect the yield of industrial ethanol fermentation," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Sheng, Haoqiang & Huang, Xiaobin & Hu, Wenbin & Ji, Yuan & Chen, Junming & Xie, Mingyun & He, Miaoshen & Zhang, Bo & Liu, Hong, 2023. "Stability and combustion performance enhancement of ethanol/kerosene fuel by carbonized poly[cyclotriphosphazene-co-(4,4′-sulfonyldiphenol)] nanotubes via biomimetic hydrogen bonding strategy," Energy, Elsevier, vol. 282(C).

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49683-2. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.