IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-45576-6.html
   My bibliography  Save this article

Multi-molecular hyperspectral PRM-SRS microscopy

Author

Listed:
  • Wenxu Zhang

    (University of California San Diego)

  • Yajuan Li

    (University of California San Diego)

  • Anthony A. Fung

    (University of California San Diego)

  • Zhi Li

    (University of California San Diego)

  • Hongje Jang

    (University of California San Diego)

  • Honghao Zha

    (University of California San Diego)

  • Xiaoping Chen

    (Northwestern University School of Medicine)

  • Fangyuan Gao

    (University of California Irvine)

  • Jane Y. Wu

    (Northwestern University School of Medicine)

  • Huaxin Sheng

    (Duke University School of Medicine)

  • Junjie Yao

    (Duke University)

  • Dorota Skowronska-Krawczyk

    (University of California Irvine)

  • Sanjay Jain

    (Washington University in St. Louis
    Washington University in St. Louis
    Washington University in St. Louis)

  • Lingyan Shi

    (University of California San Diego)

Abstract

Lipids play crucial roles in many biological processes. Mapping spatial distributions and examining the metabolic dynamics of different lipid subtypes in cells and tissues are critical to better understanding their roles in aging and diseases. Commonly used imaging methods (such as mass spectrometry-based, fluorescence labeling, conventional optical imaging) can disrupt the native environment of cells/tissues, have limited spatial or spectral resolution, or cannot distinguish different lipid subtypes. Here we present a hyperspectral imaging platform that integrates a Penalized Reference Matching algorithm with Stimulated Raman Scattering (PRM-SRS) microscopy. Using this platform, we visualize and identify high density lipoprotein particles in human kidney, a high cholesterol to phosphatidylethanolamine ratio inside granule cells of mouse hippocampus, and subcellular distributions of sphingosine and cardiolipin in human brain. Our PRM-SRS displays unique advantages of enhanced chemical specificity, subcellular resolution, and fast data processing in distinguishing lipid subtypes in different organs and species.

Suggested Citation

  • Wenxu Zhang & Yajuan Li & Anthony A. Fung & Zhi Li & Hongje Jang & Honghao Zha & Xiaoping Chen & Fangyuan Gao & Jane Y. Wu & Huaxin Sheng & Junjie Yao & Dorota Skowronska-Krawczyk & Sanjay Jain & Ling, 2024. "Multi-molecular hyperspectral PRM-SRS microscopy," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45576-6
    DOI: 10.1038/s41467-024-45576-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-45576-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-45576-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Blue B. Lake & Rajasree Menon & Seth Winfree & Qiwen Hu & Ricardo Melo Ferreira & Kian Kalhor & Daria Barwinska & Edgar A. Otto & Michael Ferkowicz & Dinh Diep & Nongluk Plongthongkum & Amanda Knoten , 2023. "An atlas of healthy and injured cell states and niches in the human kidney," Nature, Nature, vol. 619(7970), pages 585-594, July.
    2. Lingyan Shi & Chaogu Zheng & Yihui Shen & Zhixing Chen & Edilson S. Silveira & Luyuan Zhang & Mian Wei & Chang Liu & Carmen Sena-Tomas & Kimara Targoff & Wei Min, 2018. "Optical imaging of metabolic dynamics in animals," Nature Communications, Nature, vol. 9(1), pages 1-17, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Yueli Yang & Xueyang Bai & Fanghao Hu, 2024. "Photoswitchable polyynes for multiplexed stimulated Raman scattering microscopy with reversible light control," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Wei E. Gordon & Seungbyn Baek & Hai P. Nguyen & Yien-Ming Kuo & Rachael Bradley & Sarah L. Fong & Nayeon Kim & Alex Galazyuk & Insuk Lee & Melissa R. Ingala & Nancy B. Simmons & Tony Schountz & Lisa N, 2024. "Integrative single-cell characterization of a frugivorous and an insectivorous bat kidney and pancreas," Nature Communications, Nature, vol. 15(1), pages 1-23, December.
    3. Fennell, David A. & Thomsen, Bastian, 2021. "Tourism & animal suffering: Mapping the future," Annals of Tourism Research, Elsevier, vol. 91(C).
    4. Jian Zhao & Alex Matlock & Hongbo Zhu & Ziqi Song & Jiabei Zhu & Biao Wang & Fukai Chen & Yuewei Zhan & Zhicong Chen & Yihong Xu & Xingchen Lin & Lei Tian & Ji-Xin Cheng, 2022. "Bond-selective intensity diffraction tomography," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    5. Yashvardhan Jain & Leah L. Godwin & Sripad Joshi & Shriya Mandarapu & Trang Le & Cecilia Lindskog & Emma Lundberg & Katy Börner, 2023. "Segmenting functional tissue units across human organs using community-driven development of generalizable machine learning algorithms," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    6. Lingzhi Li & Ting Xiang & Jingjing Guo & Fan Guo & Yiting Wu & Han Feng & Jing Liu & Sibei Tao & Ping Fu & Liang Ma, 2024. "Inhibition of ACSS2-mediated histone crotonylation alleviates kidney fibrosis via IL-1β-dependent macrophage activation and tubular cell senescence," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    7. Nicolas Ledru & Parker C. Wilson & Yoshiharu Muto & Yasuhiro Yoshimura & Haojia Wu & Dian Li & Amish Asthana & Stefan G. Tullius & Sushrut S. Waikar & Giuseppe Orlando & Benjamin D. Humphreys, 2024. "Predicting proximal tubule failed repair drivers through regularized regression analysis of single cell multiomic sequencing," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    8. Yeran Bai & Carolina M. Camargo & Stella M. K. Glasauer & Raymond Gifford & Xinran Tian & Andrew P. Longhini & Kenneth S. Kosik, 2024. "Single-cell mapping of lipid metabolites using an infrared probe in human-derived model systems," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    9. Kian Kalhor & Chien-Ju Chen & Ho Suk Lee & Matthew Cai & Mahsa Nafisi & Richard Que & Carter R. Palmer & Yixu Yuan & Yida Zhang & Xuwen Li & Jinghui Song & Amanda Knoten & Blue B. Lake & Joseph P. Gau, 2024. "Mapping human tissues with highly multiplexed RNA in situ hybridization," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    10. Andreas Fønss Møller & Jesper Grud Skat Madsen, 2023. "JOINTLY: interpretable joint clustering of single-cell transcriptomes," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45576-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.