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Optical imaging of metabolic dynamics in animals

Author

Listed:
  • Lingyan Shi

    (Columbia University)

  • Chaogu Zheng

    (Columbia University)

  • Yihui Shen

    (Columbia University)

  • Zhixing Chen

    (Columbia University)

  • Edilson S. Silveira

    (Columbia University)

  • Luyuan Zhang

    (Columbia University)

  • Mian Wei

    (Columbia University)

  • Chang Liu

    (Columbia University)

  • Carmen Sena-Tomas

    (Columbia University)

  • Kimara Targoff

    (Columbia University)

  • Wei Min

    (Columbia University
    Columbia University)

Abstract

Direct visualization of metabolic dynamics in living animals with high spatial and temporal resolution is essential to understanding many biological processes. Here we introduce a platform that combines deuterium oxide (D2O) probing with stimulated Raman scattering (DO-SRS) microscopy to image in situ metabolic activities. Enzymatic incorporation of D2O-derived deuterium into macromolecules generates carbon–deuterium (C–D) bonds, which track biosynthesis in tissues and can be imaged by SRS in situ. Within the broad vibrational spectra of C–D bonds, we discover lipid-, protein-, and DNA-specific Raman shifts and develop spectral unmixing methods to obtain C–D signals with macromolecular selectivity. DO-SRS microscopy enables us to probe de novo lipogenesis in animals, image protein biosynthesis without tissue bias, and simultaneously visualize lipid and protein metabolism and reveal their different dynamics. DO-SRS microscopy, being noninvasive, universally applicable, and cost-effective, can be adapted to a broad range of biological systems to study development, tissue homeostasis, aging, and tumor heterogeneity.

Suggested Citation

  • Lingyan Shi & Chaogu Zheng & Yihui Shen & Zhixing Chen & Edilson S. Silveira & Luyuan Zhang & Mian Wei & Chang Liu & Carmen Sena-Tomas & Kimara Targoff & Wei Min, 2018. "Optical imaging of metabolic dynamics in animals," Nature Communications, Nature, vol. 9(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05401-3
    DOI: 10.1038/s41467-018-05401-3
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    Cited by:

    1. Yeran Bai & Carolina M. Camargo & Stella M. K. Glasauer & Raymond Gifford & Xinran Tian & Andrew P. Longhini & Kenneth S. Kosik, 2024. "Single-cell mapping of lipid metabolites using an infrared probe in human-derived model systems," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    2. Fennell, David A. & Thomsen, Bastian, 2021. "Tourism & animal suffering: Mapping the future," Annals of Tourism Research, Elsevier, vol. 91(C).
    3. Jian Zhao & Alex Matlock & Hongbo Zhu & Ziqi Song & Jiabei Zhu & Biao Wang & Fukai Chen & Yuewei Zhan & Zhicong Chen & Yihong Xu & Xingchen Lin & Lei Tian & Ji-Xin Cheng, 2022. "Bond-selective intensity diffraction tomography," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    4. Yueli Yang & Xueyang Bai & Fanghao Hu, 2024. "Photoswitchable polyynes for multiplexed stimulated Raman scattering microscopy with reversible light control," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    5. Wenxu Zhang & Yajuan Li & Anthony A. Fung & Zhi Li & Hongje Jang & Honghao Zha & Xiaoping Chen & Fangyuan Gao & Jane Y. Wu & Huaxin Sheng & Junjie Yao & Dorota Skowronska-Krawczyk & Sanjay Jain & Ling, 2024. "Multi-molecular hyperspectral PRM-SRS microscopy," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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