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Antiviral responses in a Jamaican fruit bat intestinal organoid model of SARS-CoV-2 infection

Author

Listed:
  • Marziah Hashimi

    (Montana State University, Department of Microbiology and Cell Biology)

  • T. Andrew Sebrell

    (Montana State University, Department of Microbiology and Cell Biology)

  • Jodi F. Hedges

    (Montana State University, Department of Microbiology and Cell Biology)

  • Deann Snyder

    (Montana State University, Department of Microbiology and Cell Biology)

  • Katrina N. Lyon

    (Montana State University, Department of Microbiology and Cell Biology)

  • Stephanie D. Byrum

    (University of Arkansas for Medical Sciences, Department of Biochemistry and Molecular Biology
    Arkansas Children’s Research Institute)

  • Samuel G. Mackintosh

    (University of Arkansas for Medical Sciences, Department of Biochemistry and Molecular Biology)

  • Dan Crowley

    (Montana State University, Department of Microbiology and Cell Biology
    Cornell University College of Veterinary Medicine)

  • Michelle D. Cherne

    (Montana State University, Department of Microbiology and Cell Biology)

  • David Skwarchuk

    (Montana State University, Department of Microbiology and Cell Biology)

  • Amanda Robison

    (Montana State University, Department of Microbiology and Cell Biology)

  • Barkan Sidar

    (Montana State University, Chemical and Biological Engineering Department
    Center for Biofilm Engineering)

  • Anja Kunze

    (Montana State University, Electrical and Computer Engineering Department)

  • Emma K. Loveday

    (Montana State University, Chemical and Biological Engineering Department
    Center for Biofilm Engineering)

  • Matthew P. Taylor

    (Montana State University, Department of Microbiology and Cell Biology)

  • Connie B. Chang

    (Montana State University, Chemical and Biological Engineering Department
    Center for Biofilm Engineering
    Mayo Clinic)

  • James N. Wilking

    (Montana State University, Chemical and Biological Engineering Department
    Center for Biofilm Engineering
    Mayo Clinic)

  • Seth T. Walk

    (Montana State University, Department of Microbiology and Cell Biology)

  • Tony Schountz

    (Colorado State University, Fort)

  • Mark A. Jutila

    (Montana State University, Department of Microbiology and Cell Biology)

  • Diane Bimczok

    (Montana State University, Department of Microbiology and Cell Biology
    Center for Biofilm Engineering)

Abstract

Bats are natural reservoirs for several zoonotic viruses, potentially due to an enhanced capacity to control viral infection. However, the mechanisms of antiviral responses in bats are poorly defined. Here we established a Jamaican fruit bat (JFB, Artibeus jamaicensis) intestinal organoid model of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. Upon infection with SARS-CoV-2, increased viral RNA and subgenomic RNA was detected, but no infectious virus was released, indicating that JFB organoids support only limited viral replication but not viral reproduction. SARS-CoV-2 replication was associated with significantly increased gene expression of type I interferons and inflammatory cytokines. Interestingly, SARS-CoV-2 also caused enhanced formation and growth of JFB organoids. Proteomics revealed an increase in inflammatory signaling, cell turnover, cell repair, and SARS-CoV-2 infection pathways. Collectively, our findings suggest that primary JFB intestinal epithelial cells mount successful antiviral interferon responses and that SARS-CoV-2 infection in JFB cells induces protective regenerative pathways.

Suggested Citation

  • Marziah Hashimi & T. Andrew Sebrell & Jodi F. Hedges & Deann Snyder & Katrina N. Lyon & Stephanie D. Byrum & Samuel G. Mackintosh & Dan Crowley & Michelle D. Cherne & David Skwarchuk & Amanda Robison , 2023. "Antiviral responses in a Jamaican fruit bat intestinal organoid model of SARS-CoV-2 infection," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-42610-x
    DOI: 10.1038/s41467-023-42610-x
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