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Microengineered devices enable long-term imaging of the ventral nerve cord in behaving adult Drosophila

Author

Listed:
  • Laura Hermans

    (Brain Mind Institute & Institute of Bioengineering, EPFL
    Institute of Mechanical Engineering & Institute of Bioengineering, EPFL)

  • Murat Kaynak

    (Institute of Mechanical Engineering & Institute of Bioengineering, EPFL)

  • Jonas Braun

    (Brain Mind Institute & Institute of Bioengineering, EPFL)

  • Victor Lobato Ríos

    (Brain Mind Institute & Institute of Bioengineering, EPFL)

  • Chin-Lin Chen

    (Brain Mind Institute & Institute of Bioengineering, EPFL)

  • Adam Friedberg

    (Brain Mind Institute & Institute of Bioengineering, EPFL)

  • Semih Günel

    (Brain Mind Institute & Institute of Bioengineering, EPFL
    Computer Vision Laboratory, EPFL)

  • Florian Aymanns

    (Brain Mind Institute & Institute of Bioengineering, EPFL)

  • Mahmut Selman Sakar

    (Institute of Mechanical Engineering & Institute of Bioengineering, EPFL)

  • Pavan Ramdya

    (Brain Mind Institute & Institute of Bioengineering, EPFL)

Abstract

The dynamics and connectivity of neural circuits continuously change on timescales ranging from milliseconds to an animal’s lifetime. Therefore, to understand biological networks, minimally invasive methods are required to repeatedly record them in behaving animals. Here we describe a suite of devices that enable long-term optical recordings of the adult Drosophila melanogaster ventral nerve cord (VNC). These consist of transparent, numbered windows to replace thoracic exoskeleton, compliant implants to displace internal organs, a precision arm to assist implantation, and a hinged stage to repeatedly tether flies. To validate and illustrate our toolkit we (i) show minimal impact on animal behavior and survival, (ii) follow the degradation of chordotonal organ mechanosensory nerve terminals over weeks after leg amputation, and (iii) uncover waves of neural activity caffeine ingestion. Thus, our long-term imaging toolkit opens up the investigation of premotor and motor circuit adaptations in response to injury, drug ingestion, aging, learning, and disease.

Suggested Citation

  • Laura Hermans & Murat Kaynak & Jonas Braun & Victor Lobato Ríos & Chin-Lin Chen & Adam Friedberg & Semih Günel & Florian Aymanns & Mahmut Selman Sakar & Pavan Ramdya, 2022. "Microengineered devices enable long-term imaging of the ventral nerve cord in behaving adult Drosophila," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32571-y
    DOI: 10.1038/s41467-022-32571-y
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    References listed on IDEAS

    as
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