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Homogeneous surrogate virus neutralization assay to rapidly assess neutralization activity of anti-SARS-CoV-2 antibodies

Author

Listed:
  • Sun Jin Kim

    (University of Utah)

  • Zhong Yao

    (University of Toronto)

  • Morgan C. Marsh

    (University of Utah)

  • Debra M. Eckert

    (University of Utah School of Medicine)

  • Michael S. Kay

    (University of Utah School of Medicine)

  • Anna Lyakisheva

    (University of Toronto)

  • Maria Pasic

    (University of Toronto
    St. Joseph’s Health Centre
    University of Toronto)

  • Aiyush Bansal

    (University of Toronto)

  • Chaim Birnboim

    (University of Toronto)

  • Prabhat Jha

    (University of Toronto)

  • Yannick Galipeau

    (University of Ottawa)

  • Marc-André Langlois

    (University of Ottawa
    University of Ottawa Centre for Infection, Immunity and Inflammation (CI3))

  • Julio C. Delgado

    (ARUP Institute for Clinical and Experimental Pathology
    University of Utah)

  • Marc G. Elgort

    (ARUP Institute for Clinical and Experimental Pathology)

  • Robert A. Campbell

    (University of Utah
    University of Utah
    University of Utah)

  • Elizabeth A. Middleton

    (University of Utah
    University of Utah
    University of Utah)

  • Igor Stagljar

    (University of Toronto
    University of Toronto
    University of Toronto
    Mediterranean Institute for Life Sciences)

  • Shawn C. Owen

    (University of Utah
    University of Utah
    University of Utah)

Abstract

The COVID-19 pandemic triggered the development of numerous diagnostic tools to monitor infection and to determine immune response. Although assays to measure binding antibodies against SARS-CoV-2 are widely available, more specific tests measuring neutralization activities of antibodies are immediately needed to quantify the extent and duration of protection that results from infection or vaccination. We previously developed a ‘Serological Assay based on a Tri-part split-NanoLuc® (SATiN)’ to detect antibodies that bind to the spike (S) protein of SARS-CoV-2. Here, we expand on our previous work and describe a reconfigured version of the SATiN assay, called Neutralization SATiN (Neu-SATiN), which measures neutralization activity of antibodies directly from convalescent or vaccinated sera. The results obtained with our assay and other neutralization assays are comparable but with significantly shorter preparation and run time for Neu-SATiN. As the assay is modular, we further demonstrate that Neu-SATiN enables rapid assessment of the effectiveness of vaccines and level of protection against existing SARS-CoV-2 variants of concern and can therefore be readily adapted for emerging variants.

Suggested Citation

  • Sun Jin Kim & Zhong Yao & Morgan C. Marsh & Debra M. Eckert & Michael S. Kay & Anna Lyakisheva & Maria Pasic & Aiyush Bansal & Chaim Birnboim & Prabhat Jha & Yannick Galipeau & Marc-André Langlois & J, 2022. "Homogeneous surrogate virus neutralization assay to rapidly assess neutralization activity of anti-SARS-CoV-2 antibodies," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31300-9
    DOI: 10.1038/s41467-022-31300-9
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