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Non-canonical Wnt signaling promotes directed migration of intestinal stem cells to sites of injury

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Listed:
  • Daniel Jun-Kit Hu

    (Genentech Inc.)

  • Jina Yun

    (Genentech Inc.)

  • Justin Elstrott

    (Genentech Inc.)

  • Heinrich Jasper

    (Genentech Inc.)

Abstract

Tissue regeneration after injury requires coordinated regulation of stem cell activation, division, and daughter cell differentiation, processes that are increasingly well understood in many regenerating tissues. How accurate stem cell positioning and localized integration of new cells into the damaged epithelium are achieved, however, remains unclear. Here, we show that enteroendocrine cells coordinate stem cell migration towards a wound in the Drosophila intestinal epithelium. In response to injury, enteroendocrine cells release the N-terminal domain of the PTK7 orthologue, Otk, which activates non-canonical Wnt signaling in intestinal stem cells, promoting actin-based protrusion formation and stem cell migration towards a wound. We find that this migratory behavior is closely linked to proliferation, and that it is required for efficient tissue repair during injury. Our findings highlight the role of non-canonical Wnt signaling in regeneration of the intestinal epithelium, and identify enteroendocrine cell-released ligands as critical coordinators of intestinal stem cell migration.

Suggested Citation

  • Daniel Jun-Kit Hu & Jina Yun & Justin Elstrott & Heinrich Jasper, 2021. "Non-canonical Wnt signaling promotes directed migration of intestinal stem cells to sites of injury," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27384-4
    DOI: 10.1038/s41467-021-27384-4
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    References listed on IDEAS

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    1. Craig A. Micchelli & Norbert Perrimon, 2006. "Evidence that stem cells reside in the adult Drosophila midgut epithelium," Nature, Nature, vol. 439(7075), pages 475-479, January.
    2. Mariaceleste Aragona & Sophie Dekoninck & Steffen Rulands & Sandrine Lenglez & Guilhem Mascré & Benjamin D. Simons & Cédric Blanpain, 2017. "Defining stem cell dynamics and migration during wound healing in mouse skin epidermis," Nature Communications, Nature, vol. 8(1), pages 1-14, April.
    3. Benjamin Ohlstein & Allan Spradling, 2006. "The adult Drosophila posterior midgut is maintained by pluripotent stem cells," Nature, Nature, vol. 439(7075), pages 470-474, January.
    4. Jiae Lee & Alejandra J. H. Cabrera & Cecilia M. T. Nguyen & Young V. Kwon, 2020. "Dissemination of RasV12-transformed cells requires the mechanosensitive channel Piezo," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    5. Xiaowei Lu & Annette G. M. Borchers & Christine Jolicoeur & Helen Rayburn & Julie C. Baker & Marc Tessier-Lavigne, 2004. "PTK7/CCK-4 is a novel regulator of planar cell polarity in vertebrates," Nature, Nature, vol. 430(6995), pages 93-98, July.
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    Cited by:

    1. Jina Yun & Simon Hansen & Otto Morris & David T. Madden & Clare Peters Libeu & Arjun J. Kumar & Cameron Wehrfritz & Aaron H. Nile & Yingnan Zhang & Lijuan Zhou & Yuxin Liang & Zora Modrusan & Michelle, 2023. "Senescent cells perturb intestinal stem cell differentiation through Ptk7 induced noncanonical Wnt and YAP signaling," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    2. Ana-Beatriz F. Barletta & Jamie C. Smith & Emily Burkart & Simon Bondarenko & Igor V. Sharakhov & Frank Criscione & David O’Brochta & Carolina Barillas-Mury, 2024. "Mosquito midgut stem cell cellular defense response limits Plasmodium parasite infection," Nature Communications, Nature, vol. 15(1), pages 1-9, December.

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