IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-27180-0.html
   My bibliography  Save this article

Spike residue 403 affects binding of coronavirus spikes to human ACE2

Author

Listed:
  • Fabian Zech

    (Institute of Molecular Virology, Ulm University Medical Center)

  • Daniel Schniertshauer

    (Institute of Molecular Virology, Ulm University Medical Center)

  • Christoph Jung

    (Institute of Electrochemistry, Ulm University
    Helmholtz-Institute Ulm (HIU) Electrochemical Energy Storage, Helmholtz-Straße 16
    Karlsruhe Institute of Technology (KIT), P.O. Box 3640)

  • Alexandra Herrmann

    (Institute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg)

  • Arne Cordsmeier

    (Institute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg)

  • Qinya Xie

    (Institute of Molecular Virology, Ulm University Medical Center)

  • Rayhane Nchioua

    (Institute of Molecular Virology, Ulm University Medical Center)

  • Caterina Prelli Bozzo

    (Institute of Molecular Virology, Ulm University Medical Center)

  • Meta Volcic

    (Institute of Molecular Virology, Ulm University Medical Center)

  • Lennart Koepke

    (Institute of Molecular Virology, Ulm University Medical Center)

  • Janis A. Müller

    (Institute of Molecular Virology, Ulm University Medical Center)

  • Jana Krüger

    (Ulm University Medical Center)

  • Sandra Heller

    (Ulm University Medical Center)

  • Steffen Stenger

    (Institute of Medical Microbiology and Hygiene, Ulm University Medical Centre)

  • Markus Hoffmann

    (Infection Biology Unit, German Primate Center—Leibniz Institute for Primate Research)

  • Stefan Pöhlmann

    (Infection Biology Unit, German Primate Center—Leibniz Institute for Primate Research)

  • Alexander Kleger

    (Ulm University Medical Center)

  • Timo Jacob

    (Institute of Electrochemistry, Ulm University
    Helmholtz-Institute Ulm (HIU) Electrochemical Energy Storage, Helmholtz-Straße 16
    Karlsruhe Institute of Technology (KIT), P.O. Box 3640)

  • Karl-Klaus Conzelmann

    (Max von Pettenkofer-Institute of Virology, Medical Faculty, and Gene Center, Ludwig-Maximilians-Universität München)

  • Armin Ensser

    (Institute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg)

  • Konstantin M. J. Sparrer

    (Institute of Molecular Virology, Ulm University Medical Center)

  • Frank Kirchhoff

    (Institute of Molecular Virology, Ulm University Medical Center)

Abstract

The bat sarbecovirus RaTG13 is a close relative of SARS-CoV-2, the cause of the COVID-19 pandemic. However, this bat virus was most likely unable to directly infect humans since its Spike (S) protein does not interact efficiently with the human ACE2 receptor. Here, we show that a single T403R mutation increases binding of RaTG13 S to human ACE2 and allows VSV pseudoparticle infection of human lung cells and intestinal organoids. Conversely, mutation of R403T in the SARS-CoV-2 S reduces pseudoparticle infection and viral replication. The T403R RaTG13 S is neutralized by sera from individuals vaccinated against COVID-19 indicating that vaccination might protect against future zoonoses. Our data suggest that a positively charged amino acid at position 403 in the S protein is critical for efficient utilization of human ACE2 by S proteins of bat coronaviruses. This finding could help to better predict the zoonotic potential of animal coronaviruses.

Suggested Citation

  • Fabian Zech & Daniel Schniertshauer & Christoph Jung & Alexandra Herrmann & Arne Cordsmeier & Qinya Xie & Rayhane Nchioua & Caterina Prelli Bozzo & Meta Volcic & Lennart Koepke & Janis A. Müller & Jan, 2021. "Spike residue 403 affects binding of coronavirus spikes to human ACE2," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27180-0
    DOI: 10.1038/s41467-021-27180-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-27180-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-27180-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Peng Zhou & Xing-Lou Yang & Xian-Guang Wang & Ben Hu & Lei Zhang & Wei Zhang & Hao-Rui Si & Yan Zhu & Bei Li & Chao-Lin Huang & Hui-Dong Chen & Jing Chen & Yun Luo & Hua Guo & Ren-Di Jiang & Mei-Qin L, 2020. "Addendum: A pneumonia outbreak associated with a new coronavirus of probable bat origin," Nature, Nature, vol. 588(7836), pages 6-6, December.
    2. Peng Zhou & Xing-Lou Yang & Xian-Guang Wang & Ben Hu & Lei Zhang & Wei Zhang & Hao-Rui Si & Yan Zhu & Bei Li & Chao-Lin Huang & Hui-Dong Chen & Jing Chen & Yun Luo & Hua Guo & Ren-Di Jiang & Mei-Qin L, 2020. "A pneumonia outbreak associated with a new coronavirus of probable bat origin," Nature, Nature, vol. 579(7798), pages 270-273, March.
    3. Fan Wu & Su Zhao & Bin Yu & Yan-Mei Chen & Wen Wang & Zhi-Gang Song & Yi Hu & Zhao-Wu Tao & Jun-Hua Tian & Yuan-Yuan Pei & Ming-Li Yuan & Yu-Ling Zhang & Fa-Hui Dai & Yi Liu & Qi-Min Wang & Jiao-Jiao , 2020. "A new coronavirus associated with human respiratory disease in China," Nature, Nature, vol. 579(7798), pages 265-269, March.
    4. Xiuyuan Ou & Yan Liu & Xiaobo Lei & Pei Li & Dan Mi & Lili Ren & Li Guo & Ruixuan Guo & Ting Chen & Jiaxin Hu & Zichun Xiang & Zhixia Mu & Xing Chen & Jieyong Chen & Keping Hu & Qi Jin & Jianwei Wang , 2020. "Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    5. Jian Shang & Gang Ye & Ke Shi & Yushun Wan & Chuming Luo & Hideki Aihara & Qibin Geng & Ashley Auerbach & Fang Li, 2020. "Structural basis of receptor recognition by SARS-CoV-2," Nature, Nature, vol. 581(7807), pages 221-224, May.
    6. Tommy Tsan-Yuk Lam & Na Jia & Ya-Wei Zhang & Marcus Ho-Hin Shum & Jia-Fu Jiang & Hua-Chen Zhu & Yi-Gang Tong & Yong-Xia Shi & Xue-Bing Ni & Yun-Shi Liao & Wen-Juan Li & Bao-Gui Jiang & Wei Wei & Ting-, 2020. "Identifying SARS-CoV-2-related coronaviruses in Malayan pangolins," Nature, Nature, vol. 583(7815), pages 282-285, July.
    7. Fan Wu & Su Zhao & Bin Yu & Yan-Mei Chen & Wen Wang & Zhi-Gang Song & Yi Hu & Zhao-Wu Tao & Jun-Hua Tian & Yuan-Yuan Pei & Ming-Li Yuan & Yu-Ling Zhang & Fa-Hui Dai & Yi Liu & Qi-Min Wang & Jiao-Jiao , 2020. "Author Correction: A new coronavirus associated with human respiratory disease in China," Nature, Nature, vol. 580(7803), pages 7-7, April.
    8. Wenhui Li & Michael J. Moore & Natalya Vasilieva & Jianhua Sui & Swee Kee Wong & Michael A. Berne & Mohan Somasundaran & John L. Sullivan & Katherine Luzuriaga & Thomas C. Greenough & Hyeryun Choe & M, 2003. "Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus," Nature, Nature, vol. 426(6965), pages 450-454, November.
    9. Kangpeng Xiao & Junqiong Zhai & Yaoyu Feng & Niu Zhou & Xu Zhang & Jie-Jian Zou & Na Li & Yaqiong Guo & Xiaobing Li & Xuejuan Shen & Zhipeng Zhang & Fanfan Shu & Wanyi Huang & Yu Li & Ziding Zhang & R, 2020. "Isolation of SARS-CoV-2-related coronavirus from Malayan pangolins," Nature, Nature, vol. 583(7815), pages 286-289, July.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Dongsheng Chen & Jian Sun & Jiacheng Zhu & Xiangning Ding & Tianming Lan & Xiran Wang & Weiying Wu & Zhihua Ou & Linnan Zhu & Peiwen Ding & Haoyu Wang & Lihua Luo & Rong Xiang & Xiaoling Wang & Jiayin, 2021. "Single cell atlas for 11 non-model mammals, reptiles and birds," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    2. Peter Radvak & Hyung-Joon Kwon & Martina Kosikova & Uriel Ortega-Rodriguez & Ruoxuan Xiang & Je-Nie Phue & Rong-Fong Shen & James Rozzelle & Neeraj Kapoor & Taylor Rabara & Jeff Fairman & Hang Xie, 2021. "SARS-CoV-2 B.1.1.7 (alpha) and B.1.351 (beta) variants induce pathogenic patterns in K18-hACE2 transgenic mice distinct from early strains," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    3. Naoko Iwata-Yoshikawa & Masatoshi Kakizaki & Nozomi Shiwa-Sudo & Takashi Okura & Maino Tahara & Shuetsu Fukushi & Ken Maeda & Miyuki Kawase & Hideki Asanuma & Yuriko Tomita & Ikuyo Takayama & Shutoku , 2022. "Essential role of TMPRSS2 in SARS-CoV-2 infection in murine airways," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    4. Zepeng Xu & Xinrui Kang & Pu Han & Pei Du & Linjie Li & Anqi Zheng & Chuxia Deng & Jianxun Qi & Xin Zhao & Qihui Wang & Kefang Liu & George Fu Gao, 2022. "Binding and structural basis of equine ACE2 to RBDs from SARS-CoV, SARS-CoV-2 and related coronaviruses," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    5. Hengrui Liu & Sho Iketani & Arie Zask & Nisha Khanizeman & Eva Bednarova & Farhad Forouhar & Brandon Fowler & Seo Jung Hong & Hiroshi Mohri & Manoj S. Nair & Yaoxing Huang & Nicholas E. S. Tay & Sumin, 2022. "Development of optimized drug-like small molecule inhibitors of the SARS-CoV-2 3CL protease for treatment of COVID-19," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    6. Eugene Song & Jae-Eun Lee & Seola Kwon, 2021. "Effect of Public Empathy with Infection-Control Guidelines on Infection-Prevention Attitudes and Behaviors: Based on the Case of COVID-19," IJERPH, MDPI, vol. 18(24), pages 1-18, December.
    7. Jaeyong Lee & Calem Kenward & Liam J. Worrall & Marija Vuckovic & Francesco Gentile & Anh-Tien Ton & Myles Ng & Artem Cherkasov & Natalie C. J. Strynadka & Mark Paetzel, 2022. "X-ray crystallographic characterization of the SARS-CoV-2 main protease polyprotein cleavage sites essential for viral processing and maturation," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    8. Maria de Lourdes Aguiar-Oliveira & Aline Campos & Aline R. Matos & Caroline Rigotto & Adriana Sotero-Martins & Paulo F. P. Teixeira & Marilda M. Siqueira, 2020. "Wastewater-Based Epidemiology (WBE) and Viral Detection in Polluted Surface Water: A Valuable Tool for COVID-19 Surveillance—A Brief Review," IJERPH, MDPI, vol. 17(24), pages 1-19, December.
    9. Yongin Choi & James Slghee Kim & Heejin Choi & Hyojung Lee & Chang Hyeong Lee, 2020. "Assessment of Social Distancing for Controlling COVID-19 in Korea: An Age-Structured Modeling Approach," IJERPH, MDPI, vol. 17(20), pages 1-16, October.
    10. Bruno Palialol & Paula Pereda & Carlos Azzoni, 2020. "Does weather influence COVID‐19 transmission?," Regional Science Policy & Practice, Wiley Blackwell, vol. 12(6), pages 981-1004, December.
    11. Xiaoming Hu & Shuang Wang & Shaotong Fu & Meng Qin & Chengliang Lyu & Zhaowen Ding & Yan Wang & Yishu Wang & Dongshu Wang & Li Zhu & Tao Jiang & Jing Sun & Hui Ding & Jie Wu & Lingqian Chang & Yimin C, 2023. "Intranasal mask for protecting the respiratory tract against viral aerosols," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    12. Zexun Chen & Sean Kelty & Alexandre G. Evsukoff & Brooke Foucault Welles & James Bagrow & Ronaldo Menezes & Gourab Ghoshal, 2022. "Contrasting social and non-social sources of predictability in human mobility," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    13. Frederick Ahen, 2022. "Community-Level Health Interventions are Crucial in the Post-COVID-19 Era: Lessons from Africa’s Proactive Public Health Policy Interventions," Humanistic Management Journal, Springer, vol. 7(3), pages 369-390, December.
    14. Muhammad Moazzam & Muhammad Imran Sajid & Hamza Shahid & Jahanzaib Butt & Irfan Bashir & Muhammad Jamshaid & Amir Nasrolahi Shirazi & Rakesh Kumar Tiwari, 2020. "Understanding COVID-19: From Origin to Potential Therapeutics," IJERPH, MDPI, vol. 17(16), pages 1-22, August.
    15. Malik Sallam & Deema Dababseh & Alaa’ Yaseen & Ayat Al-Haidar & Nidaa A. Ababneh & Faris G. Bakri & Azmi Mahafzah, 2020. "Conspiracy Beliefs Are Associated with Lower Knowledge and Higher Anxiety Levels Regarding COVID-19 among Students at the University of Jordan," IJERPH, MDPI, vol. 17(14), pages 1-15, July.
    16. Rafida Razali & Vijay Kumar Subbiah & Cahyo Budiman, 2021. "Technical Data of Heterologous Expression and Purification of SARS-CoV-2 Proteases Using Escherichia coli System," Data, MDPI, vol. 6(9), pages 1-13, September.
    17. Beate Jahn & Sarah Friedrich & Joachim Behnke & Joachim Engel & Ursula Garczarek & Ralf Münnich & Markus Pauly & Adalbert Wilhelm & Olaf Wolkenhauer & Markus Zwick & Uwe Siebert & Tim Friede, 2022. "On the role of data, statistics and decisions in a pandemic," AStA Advances in Statistical Analysis, Springer;German Statistical Society, vol. 106(3), pages 349-382, September.
    18. Jane Lee Jia Jing & Thong Pei Yi & Rajendran J. C. Bose & Jason R. McCarthy & Nagendran Tharmalingam & Thiagarajan Madheswaran, 2020. "Hand Sanitizers: A Review on Formulation Aspects, Adverse Effects, and Regulations," IJERPH, MDPI, vol. 17(9), pages 1-17, May.
    19. Sourya Subhra Nasker & Ananya Nanda & Balamurugan Ramadass & Sasmita Nayak, 2021. "Epidemiological Analysis of SARS-CoV-2 Transmission Dynamics in the State of Odisha, India: A Yearlong Exploratory Data Analysis," IJERPH, MDPI, vol. 18(21), pages 1-13, October.
    20. Tomokazu Yamaguchi & Midori Hoshizaki & Takafumi Minato & Satoru Nirasawa & Masamitsu N. Asaka & Mayumi Niiyama & Masaki Imai & Akihiko Uda & Jasper Fuk-Woo Chan & Saori Takahashi & Jianbo An & Akari , 2021. "ACE2-like carboxypeptidase B38-CAP protects from SARS-CoV-2-induced lung injury," Nature Communications, Nature, vol. 12(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27180-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.