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Binding and structural basis of equine ACE2 to RBDs from SARS-CoV, SARS-CoV-2 and related coronaviruses

Author

Listed:
  • Zepeng Xu

    (Chinese Academy of Sciences
    University of Macau)

  • Xinrui Kang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Pu Han

    (Chinese Academy of Sciences)

  • Pei Du

    (Chinese Academy of Sciences)

  • Linjie Li

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Anqi Zheng

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Chuxia Deng

    (University of Macau)

  • Jianxun Qi

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Xin Zhao

    (Chinese Academy of Sciences)

  • Qihui Wang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Kefang Liu

    (Chinese Academy of Sciences)

  • George Fu Gao

    (Chinese Academy of Sciences)

Abstract

The origin and host range of SARS-CoV-2, the causative agent of coronavirus disease 2019 (COVID-19), are important scientific questions as they might provide insight into understanding of the potential future spillover to infect humans. Here, we tested the binding between equine angiotensin converting enzyme 2 (eqACE2) and the receptor binding domains (RBDs) of SARS-CoV, SARS-CoV-2 prototype (PT) and variant of concerns (VOCs), as well as their close relatives bat-origin coronavirus (CoV) RaTG13 and pangolin-origin CoVs GX/P2V/2017 and GD/1/2019. We also determined the crystal structures of eqACE2/RaTG13-RBD, eqACE2/SARS-CoV-2 PT-RBD and eqACE2/Omicron BA.1-RBD. We identified S494 of SARS-COV-2 PT-RBD as an important residue in the eqACE2/SARS-COV-2 PT-RBD interaction and found that N501Y, the commonly recognized enhancing mutation, attenuated the binding affinity with eqACE2. Our work demonstrates that horses are potential targets for SARS-CoV-2 and highlights the importance of continuous surveillance on SARS-CoV-2 and related CoVs to prevent spillover events.

Suggested Citation

  • Zepeng Xu & Xinrui Kang & Pu Han & Pei Du & Linjie Li & Anqi Zheng & Chuxia Deng & Jianxun Qi & Xin Zhao & Qihui Wang & Kefang Liu & George Fu Gao, 2022. "Binding and structural basis of equine ACE2 to RBDs from SARS-CoV, SARS-CoV-2 and related coronaviruses," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31276-6
    DOI: 10.1038/s41467-022-31276-6
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