IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-26168-0.html
   My bibliography  Save this article

Self-assembled multifunctional neural probes for precise integration of optogenetics and electrophysiology

Author

Listed:
  • Liang Zou

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
    Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Huihui Tian

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology)

  • Shouliang Guan

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
    Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Jianfei Ding

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology)

  • Lei Gao

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
    Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Jinfen Wang

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology)

  • Ying Fang

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
    Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

Abstract

Optogenetics combined with electrical recording has emerged as a powerful tool for investigating causal relationships between neural circuit activity and function. However, the size of optogenetically manipulated tissue is typically 1-2 orders of magnitude larger than that can be electrically recorded, rendering difficulty for assigning functional roles of recorded neurons. Here we report a viral vector-delivery optrode (VVD-optrode) system for precise integration of optogenetics and electrophysiology in the brain. Our system consists of flexible microelectrode filaments and fiber optics that are simultaneously self-assembled in a nanoliter-scale, viral vector-delivery polymer carrier. The highly localized delivery and neuronal expression of opsin genes at microelectrode-tissue interfaces ensure high spatial congruence between optogenetically manipulated and electrically recorded neuronal populations. We demonstrate that this multifunctional system is capable of optogenetic manipulation and electrical recording of spatially defined neuronal populations for three months, allowing precise and long-term studies of neural circuit functions.

Suggested Citation

  • Liang Zou & Huihui Tian & Shouliang Guan & Jianfei Ding & Lei Gao & Jinfen Wang & Ying Fang, 2021. "Self-assembled multifunctional neural probes for precise integration of optogenetics and electrophysiology," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26168-0
    DOI: 10.1038/s41467-021-26168-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-26168-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-26168-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Takao Someya & Zhenan Bao & George G. Malliaras, 2016. "The rise of plastic bioelectronics," Nature, Nature, vol. 540(7633), pages 379-385, December.
    2. James J. Jun & Nicholas A. Steinmetz & Joshua H. Siegle & Daniel J. Denman & Marius Bauza & Brian Barbarits & Albert K. Lee & Costas A. Anastassiou & Alexandru Andrei & Çağatay Aydın & Mladen Barbic &, 2017. "Fully integrated silicon probes for high-density recording of neural activity," Nature, Nature, vol. 551(7679), pages 232-236, November.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Jongwoon Kim & Hengji Huang & Earl T. Gilbert & Kaiser C. Arndt & Daniel Fine English & Xiaoting Jia, 2024. "T-DOpE probes reveal sensitivity of hippocampal oscillations to cannabinoids in behaving mice," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Spencer Ward & Conor Riley & Erin M. Carey & Jenny Nguyen & Sadik Esener & Axel Nimmerjahn & Donald J. Sirbuly, 2022. "Electro-optical mechanically flexible coaxial microprobes for minimally invasive interfacing with intrinsic neural circuits," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    2. Jun Li & Corey Carlos & Hao Zhou & Jiajie Sui & Yikai Wang & Zulmari Silva-Pedraza & Fan Yang & Yutao Dong & Ziyi Zhang & Timothy A. Hacker & Bo Liu & Yanchao Mao & Xudong Wang, 2023. "Stretchable piezoelectric biocrystal thin films," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    3. repec:plo:pcbi00:1006854 is not listed on IDEAS
    4. Shailaja Akella & Peter Ledochowitsch & Joshua H. Siegle & Hannah Belski & Daniel D. Denman & Michael A. Buice & Severine Durand & Christof Koch & Shawn R. Olsen & Xiaoxuan Jia, 2025. "Deciphering neuronal variability across states reveals dynamic sensory encoding," Nature Communications, Nature, vol. 16(1), pages 1-22, December.
    5. Elisabeta Balla & Gerion Nabbefeld & Christopher Wiesbrock & Jenice Linde & Severin Graff & Simon Musall & Björn M. Kampa, 2025. "Broadband visual stimuli improve neuronal representation and sensory perception," Nature Communications, Nature, vol. 16(1), pages 1-23, December.
    6. Yao Ni & Jiaqi Liu & Hong Han & Qianbo Yu & Lu Yang & Zhipeng Xu & Chengpeng Jiang & Lu Liu & Wentao Xu, 2024. "Visualized in-sensor computing," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    7. Keundong Lee & Angelique C. Paulk & Yun Goo Ro & Daniel R. Cleary & Karen J. Tonsfeldt & Yoav Kfir & John S. Pezaris & Youngbin Tchoe & Jihwan Lee & Andrew M. Bourhis & Ritwik Vatsyayan & Joel R. Mart, 2024. "Flexible, scalable, high channel count stereo-electrode for recording in the human brain," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    8. Andrea Santoro & Federico Battiston & Maxime Lucas & Giovanni Petri & Enrico Amico, 2024. "Higher-order connectomics of human brain function reveals local topological signatures of task decoding, individual identification, and behavior," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    9. Guang Yao & Xiaoyi Mo & Shanshan Liu & Qian Wang & Maowen Xie & Wenhao Lou & Shiyan Chen & Taisong Pan & Ke Chen & Dezhong Yao & Yuan Lin, 2023. "Snowflake-inspired and blink-driven flexible piezoelectric contact lenses for effective corneal injury repair," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    10. Gustavo Della-Flora Nunes & Lindsay A. Osso & Johana A. Haynes & Lauren Conant & Michael A. Thornton & Michael E. Stockton & Katherine A. Brassell & Amanda Morris & Yessenia I. Mancha Corchado & John , 2025. "Incomplete remyelination via therapeutically enhanced oligodendrogenesis is sufficient to recover visual cortical function," Nature Communications, Nature, vol. 16(1), pages 1-22, December.
    11. Edward A. B. Horrocks & Fabio R. Rodrigues & Aman B. Saleem, 2024. "Flexible neural population dynamics govern the speed and stability of sensory encoding in mouse visual cortex," Nature Communications, Nature, vol. 15(1), pages 1-23, December.
    12. Tjitse van der Molen & Max Lim & Julian Bartram & Zhuowei Cheng & Ash Robbins & David F Parks & Linda R Petzold & Andreas Hierlemann & David Haussler & Paul K Hansma & Kenneth R Tovar & Kenneth S Kosi, 2024. "RT-Sort: An action potential propagation-based algorithm for real time spike detection and sorting with millisecond latencies," PLOS ONE, Public Library of Science, vol. 19(12), pages 1-36, December.
    13. Boris Sotomayor-Gómez & Francesco P Battaglia & Martin Vinck, 2023. "SpikeShip: A method for fast, unsupervised discovery of high-dimensional neural spiking patterns," PLOS Computational Biology, Public Library of Science, vol. 19(7), pages 1-29, July.
    14. Jérémie Sibille & Carolin Gehr & Jonathan I. Benichov & Hymavathy Balasubramanian & Kai Lun Teh & Tatiana Lupashina & Daniela Vallentin & Jens Kremkow, 2022. "High-density electrode recordings reveal strong and specific connections between retinal ganglion cells and midbrain neurons," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    15. Ilke Uguz & David Ohayon & Volkan Arslan & Rajendar Sheelamanthula & Sophie Griggs & Adel Hama & John William Stanton & Iain McCulloch & Sahika Inal & Kenneth L. Shepard, 2024. "Flexible switch matrix addressable electrode arrays with organic electrochemical transistor and pn diode technology," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    16. Jung Min Lee & Young-Woo Pyo & Yeon Jun Kim & Jin Hee Hong & Yonghyeon Jo & Wonshik Choi & Dingchang Lin & Hong-Gyu Park, 2023. "The ultra-thin, minimally invasive surface electrode array NeuroWeb for probing neural activity," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    17. Xiao Liu & Jingping Wu & Keke Qiao & Guohan Liu & Zhengjin Wang & Tongqing Lu & Zhigang Suo & Jian Hu, 2022. "Topoarchitected polymer networks expand the space of material properties," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    18. Patrick Jendritza & Frederike J. Klein & Pascal Fries, 2023. "Multi-area recordings and optogenetics in the awake, behaving marmoset," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    19. Hyeokjun Lee & Soojeong Song & Junwoo Yea & Jeongdae Ha & Saehyuck Oh & Janghwan Jekal & Myung Seok Hong & Chihyeong Won & Han Hee Jung & Hohyun Keum & Sangyoon Han & Jeong Ho Cho & Taeyoon Lee & Kyun, 2025. "Vialess heterogeneous skin patch for multimodal monitoring and stimulation," Nature Communications, Nature, vol. 16(1), pages 1-14, December.
    20. Francis Kei Masuda & Emily A. Aery Jones & Yanjun Sun & Lisa M. Giocomo, 2023. "Ketamine evoked disruption of entorhinal and hippocampal spatial maps," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    21. Camden J. MacDowell & Alexandra Libby & Caroline I. Jahn & Sina Tafazoli & Adel Ardalan & Timothy J. Buschman, 2025. "Multiplexed subspaces route neural activity across brain-wide networks," Nature Communications, Nature, vol. 16(1), pages 1-21, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26168-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.