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Seasonal and Technological Shifts of the WHO Priority Multi-Resistant Pathogens in Municipal Wastewater Treatment Plant and Its Receiving Surface Water: A Case Study

Author

Listed:
  • Łukasz Jałowiecki

    (Environmental Microbiology Unit, Institute for Ecology of Industrial Areas, 40-844 Katowice, Poland)

  • Jakub Hubeny

    (Department of Engineering of Water Protection and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury, 10-719 Olsztyn, Poland)

  • Monika Harnisz

    (Department of Engineering of Water Protection and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury, 10-719 Olsztyn, Poland)

  • Grażyna Płaza

    (Environmental Microbiology Unit, Institute for Ecology of Industrial Areas, 40-844 Katowice, Poland)

Abstract

The present study was focused on the identification of multi-resistant bacteria from the WHO priority pathogens list in the samples taken from different stages of the full-scale municipal wastewater treatment plant and receiving water. Additionally, the seasonal variations of the selected multi-resistant pathogens were analyzed in the samples. In order to the aim of the study, the metagenomic DNA from the collected samples was isolated and sequenced. The samples were collected in three campaigns (spring, summer, autumn). Metagenomic DNA was isolated by the commercial kits, according to the manufacturer’s instruction. Illumina sequencing system was employed, and the R program was used to metagenomic analysis. It was found that the wastewater samples and receiving water contained the multi-resistant bacteria from the WHO priority pathogens list. The seasonal and technological variations affected the distribution of the pathogens in the wastewater. No effect of the effluent on the pathogens in the receiving water was observed. The results indicated that antibiotic-resistant “priority pathogens” from the WHO list are there in the waste- and receiving water. Technological process and seasons effected their distribution in the environment. Metagenomic analysis can be used as sufficient tool in microbiological and human health risk assessment.

Suggested Citation

  • Łukasz Jałowiecki & Jakub Hubeny & Monika Harnisz & Grażyna Płaza, 2021. "Seasonal and Technological Shifts of the WHO Priority Multi-Resistant Pathogens in Municipal Wastewater Treatment Plant and Its Receiving Surface Water: A Case Study," IJERPH, MDPI, vol. 19(1), pages 1-13, December.
    • Handle: RePEc:gam:jijerp:v:19:y:2021:i:1:p:336-:d:713645
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    References listed on IDEAS

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    1. Shalinee Naidoo & Ademola O. Olaniran, 2013. "Treated Wastewater Effluent as a Source of Microbial Pollution of Surface Water Resources," IJERPH, MDPI, vol. 11(1), pages 1-22, December.
    2. Vanessa M. D’Costa & Christine E. King & Lindsay Kalan & Mariya Morar & Wilson W. L. Sung & Carsten Schwarz & Duane Froese & Grant Zazula & Fabrice Calmels & Regis Debruyne & G. Brian Golding & Hendri, 2011. "Antibiotic resistance is ancient," Nature, Nature, vol. 477(7365), pages 457-461, September.
    3. Xiaotong Wen & Feiyu Chen & Yixiang Lin & Hui Zhu & Fang Yuan & Duyi Kuang & Zhihui Jia & Zhaokang Yuan, 2020. "Microbial Indicators and Their Use for Monitoring Drinking Water Quality—A Review," Sustainability, MDPI, vol. 12(6), pages 1-14, March.
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