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Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica

Author

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  • I-Ching Kuan

    (Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. 3, Taipei 10452, Taiwan)

  • Chia-Chi Lee

    (Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. 3, Taipei 10452, Taiwan)

  • Bing-Hong Tsai

    (Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. 3, Taipei 10452, Taiwan)

  • Shiow-Ling Lee

    (Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. 3, Taipei 10452, Taiwan)

  • Wei-Ting Lee

    (Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. 3, Taipei 10452, Taiwan)

  • Chi-Yang Yu

    (Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. 3, Taipei 10452, Taiwan)

Abstract

We entrapped lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica, and then applied entrapped lipase to the synthesis of biodiesel with soybean oil or waste cooking oil as a feedstock. The effects of reaction temperature, substrate molar ratio (methanol/oil) and n -hexane content (w/w of oil) were evaluated using response surface methodology (RSM) combined with Box-Behnken design. The optimal reaction conditions for soybean oil were 43.6 °C, substrate molar ratio of 4.3%, and 75% n -hexane. The predicted and experimental values of biodiesel conversion were 79% and 76%, respectively. The optimal reaction conditions for waste cooking oil were 43.3 °C, substrate molar ratio of 5%, and 38% n -hexane. The predicted and experimental values of conversion were 68% and 67%, respectively. The conversion efficiency remained the same even after 1-month storage of entrapped lipase at 4 °C or room temperature.

Suggested Citation

  • I-Ching Kuan & Chia-Chi Lee & Bing-Hong Tsai & Shiow-Ling Lee & Wei-Ting Lee & Chi-Yang Yu, 2013. "Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica," Energies, MDPI, vol. 6(4), pages 1-13, April.
    • Handle: RePEc:gam:jeners:v:6:y:2013:i:4:p:2052-2064:d:24885
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    References listed on IDEAS

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    1. Editorial Article, 0. "Contents," Economics of Contemporary Russia, Regional Public Organization for Assistance to the Development of Institutions of the Department of Economics of the Russian Academy of Sciences, issue 2.
    2. Editorial Article, 0. "Contents," Economics of Contemporary Russia, Regional Public Organization for Assistance to the Development of Institutions of the Department of Economics of the Russian Academy of Sciences, issue 3.
    3. Demirbas, Ayhan, 2007. "Importance of biodiesel as transportation fuel," Energy Policy, Elsevier, vol. 35(9), pages 4661-4670, September.
    4. Editorial Article, 0. "Contents," Economics of Contemporary Russia, Regional Public Organization for Assistance to the Development of Institutions of the Department of Economics of the Russian Academy of Sciences, issue 3.
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    Cited by:

    1. Baskar, G. & Aiswarya, R., 2016. "Trends in catalytic production of biodiesel from various feedstocks," Renewable and Sustainable Energy Reviews, Elsevier, vol. 57(C), pages 496-504.
    2. Samuel Santos & Jaime Puna & João Gomes, 2020. "A Review on Bio-Based Catalysts (Immobilized Enzymes) Used for Biodiesel Production," Energies, MDPI, vol. 13(11), pages 1-19, June.

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