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Optimization of Nitrogen and Metal Ions Supplementation for Very High Gravity Bioethanol Fermentation from Sweet Sorghum Juice Using an Orthogonal Array Design

Author

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  • Orawan Deesuth

    (Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand)

  • Pattana Laopaiboon

    (Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
    Fermentation Research Center for Value Added Agricultural Products, Khon Kaen University, Khon Kaen 40002, Thailand)

  • Prasit Jaisil

    (Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand)

  • Lakkana Laopaiboon

    (Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
    Fermentation Research Center for Value Added Agricultural Products, Khon Kaen University, Khon Kaen 40002, Thailand)

Abstract

Optimization of four parameters, i.e. , zinc (Zn 2+ ), magnesium (Mg 2+ ), manganese (Mn 2+ ) and yeast extract for bioethanol production from sweet sorghum juice by Saccharomyces cerevisiae NP 01 under very high gravity (VHG, 270 g·L −1 of total sugar) conditions was performed using an L 9 (3 4 ) orthogonal array design. The fermentation was carried out at 30 °C in 500-mL air-locked Erlenmeyer flasks at the agitation rate of 100 rpm and the initial yeast cell concentration in the juice was approximately 5 × 10 7 cells·mL −1 . The results showed that the order of influence was yeast extract > Mn 2+ > Zn 2+ > Mg 2+ and the optimum nutrient concentrations for the ethanol fermentation were Zn 2+ , 0.01; Mg 2+ , 0.05; Mn 2+ , 0.04; and yeast extract, 9 g·L −1 . The verification experiments under the optimum condition clearly indicated that the metals and nitrogen supplementation improved ethanol production efficiency under the VHG fermentation conditions. The ethanol concentration ( P ), yield ( Y p/s ) and productivity ( Q p ) were 120.58 ± 0.26 g·L −1 , 0.49 ± 0.01 and 2.51 ± 0.01 g·L −1 ·h −1 , respectively, while in the control treatment (without nutrient supplement) P , Y p/s and Q p were only 93.45 ± 0.45 g·L −1 , 0.49 ± 0.00 and 1.30 ± 0.01 g·L −1 ·h −1 , respectively.

Suggested Citation

  • Orawan Deesuth & Pattana Laopaiboon & Prasit Jaisil & Lakkana Laopaiboon, 2012. "Optimization of Nitrogen and Metal Ions Supplementation for Very High Gravity Bioethanol Fermentation from Sweet Sorghum Juice Using an Orthogonal Array Design," Energies, MDPI, vol. 5(9), pages 1-20, August.
    • Handle: RePEc:gam:jeners:v:5:y:2012:i:9:p:3178-3197:d:19658
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    References listed on IDEAS

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    1. Naulchan Khongsay & Lakkana Laopaiboon & Prasit Jaisil & Pattana Laopaiboon, 2012. "Optimization of Agitation and Aeration for Very High Gravity Ethanol Fermentation from Sweet Sorghum Juice by Saccharomyces cerevisiae Using an Orthogonal Array Design," Energies, MDPI, vol. 5(3), pages 1-16, February.
    2. Liu, Ronghou & Li, Jinxia & Shen, Fei, 2008. "Refining bioethanol from stalk juice of sweet sorghum by immobilized yeast fermentation," Renewable Energy, Elsevier, vol. 33(5), pages 1130-1135.
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    Cited by:

    1. Pachaya Chan-u-tit & Lakkana Laopaiboon & Prasit Jaisil & Pattana Laopaiboon, 2013. "High Level Ethanol Production by Nitrogen and Osmoprotectant Supplementation under Very High Gravity Fermentation Conditions," Energies, MDPI, vol. 6(2), pages 1-16, February.
    2. Lakkana Laopaiboon & Suntaree Suporn & Preekamol Klanrit & Niphaphat Phukoetphim & Chalida Daengbussadee & Pattana Laopaiboon, 2021. "Novel Effective Yeast Strains and Their Performance in High Gravity and Very High Gravity Ethanol Fermentations from Sweet Sorghum Juice," Energies, MDPI, vol. 14(3), pages 1-15, January.
    3. Sureerat Suwanapong & Naulchan Khongsay & Lakkana Laopaiboon & Prasit Jaisil & Pattana Laopaiboon, 2013. "Dried Spent Yeast and Its Hydrolysate as Nitrogen Supplements for Single Batch and Repeated-Batch Ethanol Fermentation from Sweet Sorghum Juice," Energies, MDPI, vol. 6(3), pages 1-14, March.

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