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Single-Nucleotide Polymorphisms Identified within Exon 2 of Fertility-Associated Bone Morphogenetic Protein (BMP15) Gene in Three Romanian Sheep Breeds

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  • Alexandru Marius Deac

    (Faculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, Romania)

  • Adriana Sebastiana Musca

    (Faculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, Romania)

  • Stefania Dana Mesesan

    (Faculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, Romania)

  • Marius Gavril Aipatioaie

    (Faculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, Romania
    Agricultural Research and Development Station Turda, 401100 Turda, Romania)

  • Adrian Ionascu

    (Department of Genetics, Faculty of Biology, University of Bucharest, 060101 Bucharest, Romania)

  • Viorica Cosier

    (Faculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, Romania)

  • Attila Cristian Ratiu

    (Department of Genetics, Faculty of Biology, University of Bucharest, 060101 Bucharest, Romania)

  • Ileana Miclea

    (Faculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, Romania)

  • Ioan Ladosi

    (Faculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, Romania)

  • Marius Zahan

    (Faculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, Romania)

Abstract

The improvement of the reproductive traits of animals is of great interest for livestock production. Due to its positive impact on the sheep industry’s profitability, prolificacy is one of the most economically significant biological traits, showing variation between and within breeds of domestic sheep ( Ovis aries ). Different mutations in BMPR-1B , BMP15 and GDF9 genes coding for the transforming growth factor-β (TGFβ) superfamily have been shown to influence the ovulation rate and litter size. Numerous single-nucleotide polymorphisms (SNPs) in the bone morphogenetic protein 15 ( BMP15 ) gene have been linked to ewe fecundity. Using targeted PCR amplification and Sanger sequencing, we were able to identify heterozygous SNPs in exon 2 of BMP15 in three sheep breeds reared in Romania: Tsigai, Cluj Merino and Tsurcana. The sequence analysis revealed three previously documented mutations, namely the missense mutation c.755T>C (L252P), which is predicted to change the tertiary structure of the BMP15 protein, and two silent mutations, c.747T>C (P249P) and c.1047G>A (V349V). In addition, we also identified one novel silent mutation, c.825G>A (S275S). Based on our findings and publicly available data, we indicate four putative mutational hotspots within exon 2 of BMP15 that could be considered for improving the indigenous sheep breeds through targeted gene editing and SNP genotyping strategies.

Suggested Citation

  • Alexandru Marius Deac & Adriana Sebastiana Musca & Stefania Dana Mesesan & Marius Gavril Aipatioaie & Adrian Ionascu & Viorica Cosier & Attila Cristian Ratiu & Ileana Miclea & Ioan Ladosi & Marius Zah, 2023. "Single-Nucleotide Polymorphisms Identified within Exon 2 of Fertility-Associated Bone Morphogenetic Protein (BMP15) Gene in Three Romanian Sheep Breeds," Agriculture, MDPI, vol. 13(5), pages 1-14, April.
    • Handle: RePEc:gam:jagris:v:13:y:2023:i:5:p:996-:d:1137266
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    References listed on IDEAS

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    1. Jones, A.K. & Jones, D.L. & Cross, P., 2014. "The carbon footprint of lamb: Sources of variation and opportunities for mitigation," Agricultural Systems, Elsevier, vol. 123(C), pages 97-107.
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    More about this item

    Keywords

    SNPs; BMP15; FecX; Tsigai; Cluj Merino; Tsurcana; litter size; fecundity;
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