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Effectiveness of Species- and Trichothecene-Specific Primers in Monitoring Fusarium graminearum Species Complex in Small Grain–Pea Intercropping Systems

Author

Listed:
  • Vesna Župunski

    (Department of Small Grains, Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia)

  • Radivoje Jevtić

    (Department of Small Grains, Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia)

  • Milosav Grčak

    (Department of Plant Protection, Faculty of Agriculture, University of Priština–Kosovska Mitrovica, 38219 Lešak, Serbia)

  • Mirjana Lalošević

    (Department of Small Grains, Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia)

  • Branka Orbović

    (Department of Small Grains, Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia)

  • Dalibor Živanov

    (Department of Small Grains, Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia)

  • Desimir Knežević

    (Department of Plant Protection, Faculty of Agriculture, University of Priština–Kosovska Mitrovica, 38219 Lešak, Serbia)

Abstract

Tracking the distribution of Fusarium species and the detection of changes in toxin production provides epidemiological information that is essential for Fusarium head blight (FHB) management. Members of Fusarium graminearum species complex (FGSC) were characterized using species and trichothecene-specific primers. Associations between members of the FGSC, cereal crop species (wheat, rye, triticale, and oat), seeding time (winter and spring), type of cultivation (monocrop and intercrop) and chemotype grouping were investigated with multiple correspondence analysis and multiple regression modeling. We found that triticale and oat were more related to isolates classified into F. graminearum s. lato than with other isolates. In contrast, wheat and rye were more associated with F. graminearum s. stricto . Cereal crop species affected the frequencies of F. graminearum s. stricto ( p = 0.003) and F. graminearum s. lato ( p = 0.08) and unidentified isolates with morphological characteristics like those of FGSC members ( p = 0.02). The effectiveness of species-specific primers was 60.3% (Fg16F/R) and 76.2% (FgrF/FgcR), and the effectiveness of primer sets for the trichothecene genotyping of the Tri5 and Tri3 genes was 100% and 90.6%, respectively. The decrease in Fusarium -damaged kernel values in the wheat–pea intercropping system indicated that intercropping systems have the potential to control FHB.

Suggested Citation

  • Vesna Župunski & Radivoje Jevtić & Milosav Grčak & Mirjana Lalošević & Branka Orbović & Dalibor Živanov & Desimir Knežević, 2022. "Effectiveness of Species- and Trichothecene-Specific Primers in Monitoring Fusarium graminearum Species Complex in Small Grain–Pea Intercropping Systems," Agriculture, MDPI, vol. 12(6), pages 1-14, June.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:6:p:834-:d:835388
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    References listed on IDEAS

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    1. Drakopoulos, Dimitrios & Kägi, Andreas & Six, Johan & Zorn, Alexander & Wettstein, Felix E. & Bucheli, Thomas D. & Forrer, Hans-Rudolf & Vogelgsang, Susanne, 2021. "The agronomic and economic viability of innovative cropping systems to reduce Fusarium head blight and related mycotoxins in wheat," Agricultural Systems, Elsevier, vol. 192(C).
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