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Identification of a Novel QTL for Chlorate Resistance in Rice ( Oryza sativa L.)

Author

Listed:
  • Nkulu Rolly Kabange

    (Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)

  • So-Yeon Park

    (Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)

  • Dongjin Shin

    (Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)

  • So-Myeong Lee

    (Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)

  • Su-Min Jo

    (Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)

  • Youngho Kwon

    (Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)

  • Jin-Kyung Cha

    (Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)

  • You-Chun Song

    (Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)

  • Jong-Min Ko

    (Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)

  • Jong-Hee Lee

    (Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea)

Abstract

Chlorate resistance analysis is an effective approach commonly used to distinguish the genetic variation between Oryza sativa L. ssp. indica and japonica, and predict the nitrogen use efficiency (NUE). This study aimed at investigating the response of a doubled haploid (DH) population derived from anther culture of 93-11 × Milyang352 exposed to 0.1% potassium chlorate (KClO 3 ) at the seedling stage. The results revealed that the parental rice lines 93-11 ( indica ) and Milyang352 ( japonica ) showed distinctive phenotypic responses. The parental line 93-11 scored highly sensitive (0% survival) and Milyang352 scored resistant (66.7% survival) 7 days after treatment. The DH lines reflected the differential phenotypic response observed in parental lines. Interestingly, we identified a novel quantitative trait locus (QTL) for chlorate resistance on chromosome 3 (qCHR-3 , 136 cM, logarithm of the odds—LOD: 4.1) using Kompetitive Allele-Specific PCR (KASP) markers. The additive effect (−11.97) and phenotypic variation explained (PVE; 14.9%) indicated that the allele from Milyang352 explained the observed phenotypic variation. In addition, shoot growth showed a significant difference between parental lines, but not root growth. Moreover, in silico analysis identified candidate genes with diverse and interesting molecular and physiological functions. Therefore, this study suggested that the QTL qCHR-3 harbors promising candidate genes that could play a role in the regulation of nitrogen metabolism in rice.

Suggested Citation

  • Nkulu Rolly Kabange & So-Yeon Park & Dongjin Shin & So-Myeong Lee & Su-Min Jo & Youngho Kwon & Jin-Kyung Cha & You-Chun Song & Jong-Min Ko & Jong-Hee Lee, 2020. "Identification of a Novel QTL for Chlorate Resistance in Rice ( Oryza sativa L.)," Agriculture, MDPI, vol. 10(8), pages 1-16, August.
    • Handle: RePEc:gam:jagris:v:10:y:2020:i:8:p:360-:d:399508
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    References listed on IDEAS

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    1. Zhenyu Gao & Yufeng Wang & Guang Chen & Anpeng Zhang & Shenglong Yang & Lianguang Shang & Danying Wang & Banpu Ruan & Chaolei Liu & Hongzhen Jiang & Guojun Dong & Li Zhu & Jiang Hu & Guangheng Zhang &, 2019. "The indica nitrate reductase gene OsNR2 allele enhances rice yield potential and nitrogen use efficiency," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
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    Cited by:

    1. Noor Muhammad & Yating Zheng & Farhan Nabi & Guotao Yang & Sumbal Sajid & Abdul Hakeem & Xuechun Wang & Youlin Peng & Zartasha Khan & Yungao Hu, 2022. "Responses of nitrogen accumulation and translocation in five cytoplasmic hybrid rice cultivars," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 68(2), pages 73-81.

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