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Cane molasses fermentation for continuous ethanol production in an immobilized cells reactor by Saccharomyces cerevisiae

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  • Ghorbani, Farshid
  • Younesi, Habibollah
  • Esmaeili Sari, Abbas
  • Najafpour, Ghasem

Abstract

Sodium-alginate immobilized yeast was employed to produce ethanol continuously using cane molasses as a carbon source in an immobilized cell reactor (ICR). The immobilization of Saccharomyces cerevisiae was performed by entrapment of the cell cultured media harvested at exponential growth phase (16 h) with 3% sodium alginate. During the initial stage of operation, the ICR was loaded with fresh beads of mean diameter of 5.01 mm. The ethanol production was affected by the concentration of the cane molasses (50, 100 and 150 g/l), dilution rates (0.064, 0.096, 0.144 and 0.192 h−1) and hydraulic retention time (5.21, 6.94, 10.42 and 15.63 h) of the media. The pH of the feed medium was set at 4.5 and the fermentation was carried out at an ambient temperature. The maximum ethanol production, theoretical yield (YE/S), volumetric ethanol productivity (QP) and total sugar consumption was 19.15 g/l, 46.23%, 2.39 g l−1 h−1 and 96%, respectively.

Suggested Citation

  • Ghorbani, Farshid & Younesi, Habibollah & Esmaeili Sari, Abbas & Najafpour, Ghasem, 2011. "Cane molasses fermentation for continuous ethanol production in an immobilized cells reactor by Saccharomyces cerevisiae," Renewable Energy, Elsevier, vol. 36(2), pages 503-509.
    • Handle: RePEc:eee:renene:v:36:y:2011:i:2:p:503-509
    DOI: 10.1016/j.renene.2010.07.016
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    Cited by:

    1. Karagoz, Pınar & Bill, Roslyn M. & Ozkan, Melek, 2019. "Lignocellulosic ethanol production: Evaluation of new approaches, cell immobilization and reactor configurations," Renewable Energy, Elsevier, vol. 143(C), pages 741-752.
    2. Puengprasert, Punika & Chalobol, Tanida & Sinbuathong, Nusara & Srinophakhun, Penjit & Thanapimmetha, Anusith & Liu, Chen-Guang & Zhao, Xin-Qing & Sakdaronnarong, Chularat, 2020. "A combined cellulosic and starchy ethanol and biomethane production with stillage recycle and respective cost analysis," Renewable Energy, Elsevier, vol. 157(C), pages 444-455.
    3. Mishra, Abhishek & Sharma, Ajay K. & Sharma, Sumit & Bagai, Rashmi & Mathur, Anshu S. & Gupta, Ravi P. & Tuli, Deepak K., 2016. "Lignocellulosic ethanol production employing immobilized Saccharomyces cerevisiae in packed bed reactor," Renewable Energy, Elsevier, vol. 98(C), pages 57-63.
    4. Satar, Ibdal & Daud, Wan Ramli Wan & Kim, Byung Hong & Somalu, Mahendra Rao & Ghasemi, Mostafa, 2017. "Immobilized mixed-culture reactor (IMcR) for hydrogen and methane production from glucose," Energy, Elsevier, vol. 139(C), pages 1188-1196.
    5. Rattanapan, Anuchit & Limtong, Savitree & Phisalaphong, Muenduen, 2011. "Ethanol production by repeated batch and continuous fermentations of blackstrap molasses using immobilized yeast cells on thin-shell silk cocoons," Applied Energy, Elsevier, vol. 88(12), pages 4400-4404.
    6. Choi, In Seong & Lee, Yoon Gyo & Khanal, Sarmir Kumar & Park, Bok Jae & Bae, Hyeun-Jong, 2015. "A low-energy, cost-effective approach to fruit and citrus peel waste processing for bioethanol production," Applied Energy, Elsevier, vol. 140(C), pages 65-74.

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