IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v241y2025ics0960148124023723.html
   My bibliography  Save this article

A combined immobilization system for high-solids cellulosic ethanol production by simultaneous saccharification and fermentation

Author

Listed:
  • Kong, Dongdong
  • Chen, Zhiling
  • Liu, Han
  • Qi, Xiaoting
  • Liu, Yanping
  • Xu, Shi
  • Ye, Yutong
  • Li, Juanjuan
  • Liang, Peiqi
  • Wang, Shan
  • Hu, Wenhao
  • Jin, Xinyu
  • Liu, Xinchun
  • Zhang, Cheng
  • Tian, Shen

Abstract

A novel combined immobilization technology was introduced in this work to improve the efficiency and stability of a high-solids biocatalysis system. First, a nano biocatalyst based on commercial cellulase immobilized onto the Fe3O4@SiO2-APTES nanoparticles was synthesized utilizing glutaraldehyde as a chemical crosslinking agent with a final immobilization efficiency of 93.0 %. A decrease in the Vmax and Km values indicates that the nanoparticle-immobilized cellulase enzyme has an increased binding affinity for the cellulose substrate. Next, a recombinant yeast strain was constructed via yeast cell-surface immobilization with laccase and versatile peroxidase for synergistic lignin-degradation. Functional accessibility was detected by using flow cytometry and immunofluorescence microscopy. Finally, the cooperative biocatalysis of nanoparticle-immobilized cellulase and cell-surface immobilized S. cerevisiae strain for SSF were investigated. Fed-batch operation of the solids at a 30.0 % (DW, w/w) final substrate loading was implemented to manage the instantaneous concentration level of inhibitors and enhance the saccharification of undetoxified biomass. The combined immobilization produced a maximum ethanol titer of 79.5 ± 4.3 g/L and a theoretical ethanol yield of 88.2 % with a low cellulase loading of 10 FPU/g cellulose. This work presented a promising green pathway for achieving efficient cellulosic ethanol production, highlighting potential industrial applications and contributing to manufacturing optimization.

Suggested Citation

  • Kong, Dongdong & Chen, Zhiling & Liu, Han & Qi, Xiaoting & Liu, Yanping & Xu, Shi & Ye, Yutong & Li, Juanjuan & Liang, Peiqi & Wang, Shan & Hu, Wenhao & Jin, Xinyu & Liu, Xinchun & Zhang, Cheng & Tian, 2025. "A combined immobilization system for high-solids cellulosic ethanol production by simultaneous saccharification and fermentation," Renewable Energy, Elsevier, vol. 241(C).
    • Handle: RePEc:eee:renene:v:241:y:2025:i:c:s0960148124023723
    DOI: 10.1016/j.renene.2024.122304
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0960148124023723
    Download Restriction: Full text for ScienceDirect subscribers only
    File URL: https://libkey.io/10.1016/j.renene.2024.122304?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Poolakkalody, Najya Jabeen & Ramesh, Kaviraj & Palliprath, Suchithra & Nittoor, Shima Namath & Santiago, Rogelio & Kabekkodu, Shama Prasada & Manisseri, Chithra, 2023. "Understanding triethylammonium hydrogen sulfate ([TEA][HSO4]) pretreatment induced changes in Pennisetum polystachion cell wall matrix and its implications on biofuel yield," Renewable Energy, Elsevier, vol. 209(C), pages 420-430.
    2. Zhu, Xing & Tian, Yi & He, Bin, 2023. "Modification of cellulase with smart-green polymers to promote low-cost and cleaner production of cellulosic ethanol," Renewable Energy, Elsevier, vol. 205(C), pages 525-533.
    3. Du, Jiliang & Chen, Le & Li, Jianan & Zuo, Ranan & Yang, Xiushan & Chen, Hongzhang & Zhuang, Xinshu & Tian, Shen, 2018. "High-solids ethanol fermentation with single-stage methane anaerobic digestion for maximizing bioenergy conversion from a C4 grass (Pennisetum purpereum)," Applied Energy, Elsevier, vol. 215(C), pages 437-443.
    4. Yulin, Xiang & Zhang, Yongbo & Wu, Jingqi & Zhu, Jing & Cao, Baowei & Xiong, Chunyan, 2024. "Immobilization of laccase and glucosidase on TiO2/CdS nanoparticles for enhanced H2 production from Spartina alterniflora Loisel," Renewable Energy, Elsevier, vol. 235(C).
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Cheng, F. & Brewer, C.E., 2021. "Conversion of protein-rich lignocellulosic wastes to bio-energy: Review and recommendations for hydrolysis + fermentation and anaerobic digestion," Renewable and Sustainable Energy Reviews, Elsevier, vol. 146(C).
    2. Kyriakou, Maria & Chatziiona, Vasiliki K. & Costa, Costas N. & Kallis, Michalis & Koutsokeras, Loukas & Constantinides, Georgios & Koutinas, Michalis, 2019. "Biowaste-based biochar: A new strategy for fermentative bioethanol overproduction via whole-cell immobilization," Applied Energy, Elsevier, vol. 242(C), pages 480-491.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:241:y:2025:i:c:s0960148124023723. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.