IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v152y2020icp653-663.html
   My bibliography  Save this article

Sugarcane bagasse saccharification using Aspergillus tubingensis enzymatic cocktail for 2G bio-ethanol production

Author

Listed:
  • Prajapati, Bhanu Pratap
  • Jana, Uttam Kumar
  • Suryawanshi, Rahul Kumar
  • Kango, Naveen

Abstract

In order to meet the increasing demand of 2G ethanol, sugarcane bagasse (SCB), a low cost by-product of sugarcane industry, can be utilized. However, cost-effective degradation of SCB into constituent fermentable sugars and subsequent ethanolic fermentation remains to be a far-fetched goal. In the present study, cellulase and hemicellulase cocktail produced by Aspergillus tubingensis NKBP-55 was applied for SCB hydrolysis under parametrically optimized conditions. Enzyme treatment (SCB: 7%, enzyme load: 1U = 1 mg) resulted in the liberation of fermentable mono-sugars 20 mg/mL (glucose, xylose and arabinose). Powder X-ray diffraction (PXRD) analysis revealed increase in the crystallinity index from 58.4 to 63.9% as a result of degradation of hemicellulose and amorphous cellulose. SCB hydrolysate was fermented to ethanol (0.415 g/g) by glucose and xylose-fermenting yeast, Candida shehatae NCIM 3501. Maximum ethanol concentration (15.54 ± 0.3 g/L) with 77.9% fermentation efficiency and 0.161 g/L/h productivity was achieved. The present study revealed that A. tubingensis enzyme cocktail can be used for efficient SCB hydrolysis and conversion of resulting hydrolysate into bioethanol using C. shehatae.

Suggested Citation

  • Prajapati, Bhanu Pratap & Jana, Uttam Kumar & Suryawanshi, Rahul Kumar & Kango, Naveen, 2020. "Sugarcane bagasse saccharification using Aspergillus tubingensis enzymatic cocktail for 2G bio-ethanol production," Renewable Energy, Elsevier, vol. 152(C), pages 653-663.
    • Handle: RePEc:eee:renene:v:152:y:2020:i:c:p:653-663
    DOI: 10.1016/j.renene.2020.01.063
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0960148120300756
    Download Restriction: Full text for ScienceDirect subscribers only
    File URL: https://libkey.io/10.1016/j.renene.2020.01.063?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Brienzo, Michel & Fikizolo, Simphiwe & Benjamin, Yuda & Tyhoda, Luvuyo & Görgens, Johann, 2017. "Influence of pretreatment severity on structural changes, lignin content and enzymatic hydrolysis of sugarcane bagasse samples," Renewable Energy, Elsevier, vol. 104(C), pages 271-280.
    2. Patel, Harshvadan & Chapla, Digantkumar & Shah, Amita, 2017. "Bioconversion of pretreated sugarcane bagasse using enzymatic and acid followed by enzymatic hydrolysis approaches for bioethanol production," Renewable Energy, Elsevier, vol. 109(C), pages 323-331.
    3. Jain, Lavika & Agrawal, Deepti, 2018. "Performance evaluation of fungal cellulases with dilute acid pretreated sugarcane bagasse: A robust bioprospecting strategy for biofuel enzymes," Renewable Energy, Elsevier, vol. 115(C), pages 978-988.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Rosen, Yan & Mamane, Hadas & Gerchman, Yoram, 2021. "Immersed ozonation of agro-wastes as an effective pretreatment method in bioethanol production," Renewable Energy, Elsevier, vol. 174(C), pages 382-390.
    2. Vandenberghe, L.P.S. & Valladares-Diestra, K.K. & Bittencourt, G.A. & Zevallos Torres, L.A. & Vieira, S. & Karp, S.G. & Sydney, E.B. & de Carvalho, J.C. & Thomaz Soccol, V. & Soccol, C.R., 2022. "Beyond sugar and ethanol: The future of sugarcane biorefineries in Brazil," Renewable and Sustainable Energy Reviews, Elsevier, vol. 167(C).
    3. Sattar Jabbar Murad Algayyim & Talal Yusaf & Naseer H. Hamza & Andrew P. Wandel & I. M. Rizwanul Fattah & Mohamd Laimon & S. M. Ashrafur Rahman, 2022. "Sugarcane Biomass as a Source of Biofuel for Internal Combustion Engines (Ethanol and Acetone-Butanol-Ethanol): A Review of Economic Challenges," Energies, MDPI, vol. 15(22), pages 1-17, November.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Shen, Guannan & Yuan, Xinchuan & Chen, Sitong & Liu, Shuangmei & Jin, Mingjie, 2022. "High titer cellulosic ethanol production from sugarcane bagasse via DLCA pretreatment and process development without washing/detoxifying pretreated biomass," Renewable Energy, Elsevier, vol. 186(C), pages 904-913.
    2. Wang, Zhi-Wen & Zhu, Ming-Qiang & Li, Ming-Fei & Wei, Qin & Sun, Run-Cang, 2019. "Effects of hydrothermal treatment on enhancing enzymatic hydrolysis of rapeseed straw," Renewable Energy, Elsevier, vol. 134(C), pages 446-452.
    3. Joanna Berlowska & Katarzyna Pielech-Przybylska & Maria Balcerek & Weronika Cieciura & Sebastian Borowski & Dorota Kregiel, 2017. "Integrated Bioethanol Fermentation/Anaerobic Digestion for Valorization of Sugar Beet Pulp," Energies, MDPI, vol. 10(9), pages 1-16, August.
    4. Liu, Jeng-Chen & Chang, Wan-Jhu & Hsu, Teng-Chieh & Chen, Hui-Jye & Chen, Yo-Chia, 2020. "Direct fermentation of cellulose to ethanol by Saccharomyces cerevisiae displaying a bifunctional cellobiohydrolase gene from Orpinomyces sp. Y102," Renewable Energy, Elsevier, vol. 159(C), pages 1029-1035.
    5. Lv, Yanting & Chen, Zhengyu & Wang, Huan & Xiao, Yongcang & Ling, Rongxin & Gong, Murong & Wei, Weiqi, 2022. "Enhancement of glucose production from sugarcane bagasse through an HCl-catalyzed ethylene glycol pretreatment and Tween 80," Renewable Energy, Elsevier, vol. 194(C), pages 495-503.
    6. Jang, Soo-Kyeong & Choi, June-Ho & Kim, Jong-Hwa & Kim, Hoyong & Jeong, Hanseob & Choi, In-Gyu, 2020. "Statistical analysis of glucose production from Eucalyptus pellita with individual control of chemical constituents," Renewable Energy, Elsevier, vol. 148(C), pages 298-308.
    7. Zhang, Weiwei & Zhang, Xiankun & Lei, Fuhou & Jiang, Jianxin, 2020. "Co-production bioethanol and xylooligosaccharides from sugarcane bagasse via autohydrolysis pretreatment," Renewable Energy, Elsevier, vol. 162(C), pages 2297-2305.
    8. Gomes, Michelle Garcia & Gurgel, Leandro Vinícius Alves & Baffi, Milla Alves & Pasquini, Daniel, 2020. "Pretreatment of sugarcane bagasse using citric acid and its use in enzymatic hydrolysis," Renewable Energy, Elsevier, vol. 157(C), pages 332-341.
    9. Pontes, Rita & Romaní, Aloia & Michelin, Michele & Domingues, Lucília & Teixeira, José & Nunes, João, 2018. "Comparative autohydrolysis study of two mixtures of forest and marginal land resources for co-production of biofuels and value-added compounds," Renewable Energy, Elsevier, vol. 128(PA), pages 20-29.
    10. Yuan, Xinchuan & Chen, Xiangxue & Shen, Guannan & Chen, Sitong & Yu, Jianming & Zhai, Rui & Xu, Zhaoxian & Jin, Mingjie, 2022. "Densifying lignocellulosic biomass with sulfuric acid provides a durable feedstock with high digestibility and high fermentability for cellulosic ethanol production," Renewable Energy, Elsevier, vol. 182(C), pages 377-389.
    11. Lü, Fan & Hua, Zhang & Shao, Liming & He, Pinjing, 2018. "Loop bioenergy production and carbon sequestration of polymeric waste by integrating biochemical and thermochemical conversion processes: A conceptual framework and recent advances," Renewable Energy, Elsevier, vol. 124(C), pages 202-211.
    12. Rosen, Yan & Mamane, Hadas & Gerchman, Yoram, 2021. "Immersed ozonation of agro-wastes as an effective pretreatment method in bioethanol production," Renewable Energy, Elsevier, vol. 174(C), pages 382-390.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:152:y:2020:i:c:p:653-663. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.