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Cellulases from Extremophiles

Author

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  • Giorgi Kvesitadze

    (Durmishidze Institute of Biochemistry and Biotechnology of Agricultural University of Georgia 0159, Georgia)

Abstract

Enzymatic hydrolysis of cellulose to fermentable glucose is the most important technological process among all possible enzyme technologies. Thermophilic fungi are potential sources of enzymes with scientific and commercial interests. From the collection of microscophic fungi isolated from ecological niches of Georgia at S.Durmishidze institute of Biochemistry and Biotechnology of Agricultural University thermophilic micromycetes -active producers of stable cellulases has been selected. Four endoglucanases were purified to homogeneity from Sporotrichum pulverulentum, Aspergillus wentii, Aspergillus versicolor, Chaetomium thermophile culture medium. Some kinetic, physical and chemical properties of purified engodlucanases (Molecular mass, isoelectric point, carbohydrates content, pH, temperature optimums, Km, Kcat, Vmax, Ki, Henries constant Kp, substrate specificity) has been studied.

Suggested Citation

  • Giorgi Kvesitadze, 2017. "Cellulases from Extremophiles," Current Trends in Biomedical Engineering & Biosciences, Juniper Publishers Inc., vol. 4(3), pages 44-49, May.
    • Handle: RePEc:adp:jctbeb:v:4:y:2017:i:3:p:44-49
    DOI: 10.19080/CTBEB.2017.04.555640
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    References listed on IDEAS

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    1. Pabulo Henrique Rampelotto, 2010. "Resistance of Microorganisms to Extreme Environmental Conditions and Its Contribution to Astrobiology," Sustainability, MDPI, vol. 2(6), pages 1-22, June.
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