IDEAS home Printed from https://ideas.repec.org/a/adp/ijcsmb/v4y2018i1p21-24.html
   My bibliography  Save this article

Three-Dimensional Culture Model of Tumoral Cells

Author

Listed:
  • Palma Maria Belen
  • Martinez Marina
  • Luzzani Carlos

    (University of Medicine, Argentina)

  • Andrini Laura
  • Errecalde , Lía
  • Inda Ana Maria

    (LIAN laboratory, Argentina)

  • Pelinski P
  • Miriuka Santiago Gabriel
  • García Marcela Nilda

    (Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina)

Abstract

The mammospheres are a multicellular tumor spheroid model. These are a widely used culture system to mimic the three-dimensionality of tumors. In this manuscript, we narrate the developmentof a model using a murine mammary adenocarcinoma, which we used to study tumor physiology and therapeutic applications. We test three techniques published in previous works. The best condition was used V-shaped 96-well plates with an agarose monolayer, with this we were obtained compact and robust spheroids. After that, we studied the interaction between tumoral cells and mesenchymal stem cells (MSC) derived to human umbilical cord in three dimensional culture.

Suggested Citation

  • Palma Maria Belen & Martinez Marina & Luzzani Carlos & Andrini Laura & Errecalde , Lía & Inda Ana Maria & Pelinski P & Miriuka Santiago Gabriel & García Marcela Nilda, 2018. "Three-Dimensional Culture Model of Tumoral Cells," International Journal of Cell Science & Molecular Biology, Juniper Publishers Inc., vol. 4(1), pages 21-24, March.
    • Handle: RePEc:adp:ijcsmb:v:4:y:2018:i:1:p:21-24
    DOI: 10.19080/IJCSMB.2018.03.555630
    as

    Download full text from publisher

    File URL: https://juniperpublishers.com/ijcsmb/pdf/IJCSMB.MS.ID.555630.pdf
    Download Restriction: no
    File URL: https://juniperpublishers.com/ijcsmb/IJCSMB.MS.ID.555630.php
    Download Restriction: no
    File URL: https://libkey.io/10.19080/IJCSMB.2018.03.555630?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Austin, Rebekah E. & Dunham, Lee M., 2022. "Do FinTech acquisitions improve the operating performance or risk profiles of acquiring firms?," Journal of Economics and Business, Elsevier, vol. 121(C).

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:adp:ijcsmb:v:4:y:2018:i:1:p:21-24. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Robert Thomas (email available below). General contact details of provider: .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.