IDEAS home Printed from https://ideas.repec.org/a/adp/ijcsmb/v3y2017i2p30-35.html
   My bibliography  Save this article

Electrical Bioimpedance Spectroscopy as Biosensor Technique to Identify Pluripotent Stem Cells and Fibroblasts

Author

Listed:
  • Vazquez-Zapien GJ

    (Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, SEDENA, México)

  • Mata-Miranda MM

    (Escuela Superior de Medicina, Instituto Politécnico Nacional, México)

  • Guerrero-Robles CI
  • Sanchez-Monroy V

    (Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, México)

  • Lozano-Trenado LM
  • Gonzalez-Diaz CA

    (Escuela Militar de Graduados de Sanidad, Centro Militar de Ciencias de la Salud, SEDENA, México)

Abstract

Pluripotent stem cells have the capacity to differentiate into specialized cells, and it has relevance in the engineering of tissue regeneration. The identification and characterization of diverse cells types requires complex techniques as flow cytometry, immunocytochemistry and the exploration of molecular markers; such techniques require infrastructure and qualified personnel. The objective of this study was to analyse the use of Electrical Bioimpedance Spectroscopy (EBIS) measurements as non-complex alternative technique to identify populations of undifferentiated pluripotent mouse Embryonic Stem Cells (mESCs) and Mouse Embryonic Fibroblasts (MEFs). EBIS measurements were compared in populations of pluripotent stem cells and fibroblasts which were characterized previously using Fourier Transform Infrared (FTIR) spectroscopy and fluorescence microscopy. The results indicate that EBIS technique has potential sensitivity at certain frequency range to discriminate between both evaluated cell populations. Additional studies with different concentrations in order to evaluate quantitatively the sensitivity and specificity of the proposed technique are recommended.

Suggested Citation

  • Vazquez-Zapien GJ & Mata-Miranda MM & Guerrero-Robles CI & Sanchez-Monroy V & Lozano-Trenado LM & Gonzalez-Diaz CA, 2017. "Electrical Bioimpedance Spectroscopy as Biosensor Technique to Identify Pluripotent Stem Cells and Fibroblasts," International Journal of Cell Science & Molecular Biology, Juniper Publishers Inc., vol. 3(2), pages 30-35, September.
    • Handle: RePEc:adp:ijcsmb:v:3:y:2017:i:2:p:30-35
    DOI: 10.19080/IJCSMB.2017.03.555610
    as

    Download full text from publisher

    File URL: https://juniperpublishers.com/ijcsmb/pdf/IJCSMB.MS.ID.555610.pdf
    Download Restriction: no
    File URL: https://juniperpublishers.com/ijcsmb/IJCSMB.MS.ID.555610.php
    Download Restriction: no
    File URL: https://libkey.io/10.19080/IJCSMB.2017.03.555610?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Sanjay K. Singh & Mohamedi N. Kagalwala & Jan Parker-Thornburg & Henry Adams & Sadhan Majumder, 2008. "REST maintains self-renewal and pluripotency of embryonic stem cells," Nature, Nature, vol. 453(7192), pages 223-227, May.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.

      Corrections

      All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:adp:ijcsmb:v:3:y:2017:i:2:p:30-35. See general information about how to correct material in RePEc.

      If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

      If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

      If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

      For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Robert Thomas (email available below). General contact details of provider: .

      Please note that corrections may take a couple of weeks to filter through the various RePEc services.

      IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.