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Isolation and Characterization of Microalgae from Diverse Pakistani Habitats: Exploring Third-Generation Biofuel Potential

Author

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  • Muhammad Maqsood Alam

    (Département de microbiologie, infectiologie et immunologie, Université de Montréal, CP 6128, Centre-ville, Montréal, PQ H3C 3J7, Canada
    Plant Genetics and Genomics Laboratory, Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan)

  • Abdul Samad Mumtaz

    (Plant Genetics and Genomics Laboratory, Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan)

  • Megan Russell

    (Life Sciences Research Center, Department of Biology, United States Air Force Academy, Colorado Springs, CO 80840, USA)

  • Melanie Grogger

    (Life Sciences Research Center, Department of Biology, United States Air Force Academy, Colorado Springs, CO 80840, USA)

  • Don Veverka

    (Life Sciences Research Center, Department of Biology, United States Air Force Academy, Colorado Springs, CO 80840, USA)

  • Patrick C. Hallenbeck

    (Département de microbiologie, infectiologie et immunologie, Université de Montréal, CP 6128, Centre-ville, Montréal, PQ H3C 3J7, Canada
    Life Sciences Research Center, Department of Biology, United States Air Force Academy, Colorado Springs, CO 80840, USA)

Abstract

Production of microalgae as feedstock for biofuels must deal with a number of challenges including constraints imposed by local conditions. One solution is to use indigenous strains adapted to local climatic conditions. The present report describes the isolation, identification, and characterization of 32 microalgal strains from different ecological habitats: desert freshwater channels, northern region, and saline regions of Pakistan. The effects of temperature on algal growth rates, biomass productivity, and lipid content were determined through growth at 12, 20, and 35 °C for 15 days under 2% CO 2 Responses to temperature varied among species with 20 °C being the optimum temperature in general, although, exceptionally, the best overall growth rate was found for strain S29 (0.311 d −1 ) at 12 °C. In some cases high biomass productivity was observed at 35 °C, and, depending upon the strain, the maximum lipid content was obtained at different temperatures, including 12 °C. Fatty acid methyl ester (FAME) analysis showed that the major fatty acids present were palmitic, stearic, oleic, linoleic, and linolenic. Oleic acid (C18:1) was the predominant fatty acid, with the specific FAME profile varying with strain. Thus, there is a rich diversity of microalgal strains native to Pakistan, some of which, characterized here, could be suitable for biodiesel production or other biotechnological applications.

Suggested Citation

  • Muhammad Maqsood Alam & Abdul Samad Mumtaz & Megan Russell & Melanie Grogger & Don Veverka & Patrick C. Hallenbeck, 2019. "Isolation and Characterization of Microalgae from Diverse Pakistani Habitats: Exploring Third-Generation Biofuel Potential," Energies, MDPI, vol. 12(14), pages 1-17, July.
    • Handle: RePEc:gam:jeners:v:12:y:2019:i:14:p:2660-:d:247458
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    References listed on IDEAS

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    1. Van Thang Duong & Yan Li & Ekaterina Nowak & Peer M. Schenk, 2012. "Microalgae Isolation and Selection for Prospective Biodiesel Production," Energies, MDPI, vol. 5(6), pages 1-15, June.
    2. Shah, Syed Hasnain & Raja, Iftikhar Ahmed & Rizwan, Muhammad & Rashid, Naim & Mahmood, Qaisar & Shah, Fayyaz Ali & Pervez, Arshid, 2018. "Potential of microalgal biodiesel production and its sustainability perspectives in Pakistan," Renewable and Sustainable Energy Reviews, Elsevier, vol. 81(P1), pages 76-92.
    3. Singh, Anoop & Olsen, Stig Irving, 2011. "A critical review of biochemical conversion, sustainability and life cycle assessment of algal biofuels," Applied Energy, Elsevier, vol. 88(10), pages 3548-3555.
    4. Ramganesh Selvarajan & Tamás Felföldi & Tamás Tauber & Elumalai Sanniyasi & Timothy Sibanda & Memory Tekere, 2015. "Screening and Evaluation of Some Green Algal Strains (Chlorophyceae) Isolated from Freshwater and Soda Lakes for Biofuel Production," Energies, MDPI, vol. 8(7), pages 1-20, July.
    5. Odlare, M. & Nehrenheim, E. & Ribé, V. & Thorin, E. & Gavare, M. & Grube, M., 2011. "Cultivation of algae with indigenous species – Potentials for regional biofuel production," Applied Energy, Elsevier, vol. 88(10), pages 3280-3285.
    6. Hallenbeck, P.C. & Grogger, M. & Mraz, M. & Veverka, D., 2016. "Solar biofuels production with microalgae," Applied Energy, Elsevier, vol. 179(C), pages 136-145.
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