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Characterization Of Macrophomina Phas Ina Isolates Collected From The Dominican Republic And Puerto Rico

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  • Echavez-Badel, Rodrigo
  • Campo-Arana, Rodrigo
  • Betancourt, Carlos

Abstract

Charcoal rotor ashy stem blight caused by the soil-borne fungus Macrophominaphaseolina (Mp) is recognized as an important disease of bean (Phaseolus vulgaris) in Puerto Rico (PR) and the Dominican Republic (DR). Severe symptoms occur especially when bean seedlings or adult plants are stressed from moisture deficits. Differences in virulence and morphological, physiological, and biochemical characteristics were obtained among PR and DR isolates of Mp in this study. PRMp2 was more virulent than PRMpl. However, DRMp2 was more virulent than either PRMp2 or DRMpl. Mycelia morphological characteristics and sclerotia size were similar in the DR isolates but different from those of the PR isolate. There were not significant differences in sclerotia size and mycelia radial growth of each isolate under light or dark conditions. Analysis of protein patterns of the PR and DR isolates was performed by polyacrylamide gel electrophoresis (PAGE). Protein profiles of DR isolates were similar but different from that of PR isolate (PRMp2).

Suggested Citation

  • Echavez-Badel, Rodrigo & Campo-Arana, Rodrigo & Betancourt, Carlos, 2000. "Characterization Of Macrophomina Phas Ina Isolates Collected From The Dominican Republic And Puerto Rico," 36th Annual Meeting, August 27-September 1, 2000, Boca Chica, Santo Domingo, Dominican Republic 256779, Caribbean Food Crops Society.
    • Handle: RePEc:ags:cfcs00:256779
    DOI: 10.22004/ag.econ.256779
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    References listed on IDEAS

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    1. Zaumeyer, W. J. & Thomas, H. Rex, 1957. "A Monographic Study of Bean Diseases and Methods for Their Control," Technical Bulletins 169625, United States Department of Agriculture, Economic Research Service.
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