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Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells

Author

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  • Zhen-Ming Pei

    (Cell and Developmental Biology, and Center for Molecular Genetics, University of California at San Diego)

  • Yoshiyuki Murata

    (Cell and Developmental Biology, and Center for Molecular Genetics, University of California at San Diego)

  • Gregor Benning

    (Technische Universität München, Lehrstuhl für Botanik)

  • Sébastien Thomine

    (Cell and Developmental Biology, and Center for Molecular Genetics, University of California at San Diego)

  • Birgit Klüsener

    (Cell and Developmental Biology, and Center for Molecular Genetics, University of California at San Diego)

  • Gethyn J. Allen

    (Cell and Developmental Biology, and Center for Molecular Genetics, University of California at San Diego)

  • Erwin Grill

    (Technische Universität München, Lehrstuhl für Botanik)

  • Julian I. Schroeder

    (Cell and Developmental Biology, and Center for Molecular Genetics, University of California at San Diego)

Abstract

Drought is a major threat to agricultural production. Plants synthesize the hormone abscisic acid (ABA) in response to drought, triggering a signalling cascade in guard cells that results in stomatal closure, thus reducing water loss1. ABA triggers an increase in cytosolic calcium in guard cells ([Ca2+]cyt)2,3,4,5,6 that has been proposed to include Ca2+ influx across the plasma membrane3,5,7,8,9. However, direct recordings of Ca2+ currents have been limited3 and the upstream activation mechanisms of plasma membrane Ca2+ channels remain unknown. Here we report activation of Ca2+-permeable channels in the plasma membrane of Arabidopsis guard cells by hydrogen peroxide. The H2O2-activated Ca2+ channels mediate both influx of Ca2+ in protoplasts and increases in [Ca2+]cyt in intact guard cells. ABA induces the production of H2O2 in guard cells. If H2O2 production is blocked, ABA-induced closure of stomata is inhibited. Moreover, activation of Ca2+ channels by H2O2 and ABA- and H2O2-induced stomatal closing are disrupted in the recessive ABA-insensitive mutant gca2. These data indicate that ABA-induced H2O2 production and the H2O2-activated Ca2+ channels are important mechanisms for ABA-induced stomatal closing.

Suggested Citation

  • Zhen-Ming Pei & Yoshiyuki Murata & Gregor Benning & Sébastien Thomine & Birgit Klüsener & Gethyn J. Allen & Erwin Grill & Julian I. Schroeder, 2000. "Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells," Nature, Nature, vol. 406(6797), pages 731-734, August.
    • Handle: RePEc:nat:nature:v:406:y:2000:i:6797:d:10.1038_35021067
    DOI: 10.1038/35021067
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    Cited by:

    1. Ping Li & Nana Xu & Yang Shui & Jie Zhang & Wuzhong Yin & Min Tian & Faping Guo & Dasong Bai & Pan Qi & Qingxiong Huang & Biluo Li & Yuanyuan Li & Yungao Hu & Youlin Peng, 2023. "Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of SPL5 in Rice," Agriculture, MDPI, vol. 13(10), pages 1-16, September.
    2. Zhang You & Shiyuan Guo & Qiao Li & Yanjun Fang & Panpan Huang & Chuanfeng Ju & Cun Wang, 2023. "The CBL1/9-CIPK1 calcium sensor negatively regulates drought stress by phosphorylating the PYLs ABA receptor," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    3. Amandeep Kaur & Madhu & Alok Sharma & Kashmir Singh & Santosh Kumar Upadhyay, 2023. "Exploration of Piezo Channels in Bread Wheat ( Triticum aestivum L.)," Agriculture, MDPI, vol. 13(4), pages 1-16, March.
    4. Helena KYSELÁKOVÁ & Michaela SEDLÁŘOVÁ & Martin KUBALA & Vladimíra NOŽKOVÁ & Jana PITERKOVÁ & Lenka LUHOVÁ & Ondřej NOVÁK & Petr ILÍK, 2013. "Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virus," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 49(3), pages 105-119.

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