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Standardization guidelines and future trends for PET hydrolase research

Author

Listed:
  • Ren Wei

    (University of Greifswald)

  • Peter Westh

    (Technical University of Denmark)

  • Gert Weber

    (Helmholtz-Zentrum Berlin für Materialien und Energie)

  • Lars M. Blank

    (RWTH Aachen University
    RWTH Aachen University)

  • Uwe T. Bornscheuer

    (University of Greifswald)

Abstract

Enzymatic depolymerization of polyethylene terephthalate (PET) towards monomer recycling offers a green route to a circular plastic economy, with scale-up currently underway. Yet, inconsistent assessment methods hinder clear comparisons between various PET hydrolases. This Perspective aims to identify critical gaps in this dynamic research field and outline key principles for selecting and tailoring novel enzymes, such as using uniform PET samples and standardizing reaction settings that mimic industrial conditions. Applying these guidelines will improve enzyme screening efficiency, increase data reproducibility, deepen the understanding of interfacial biocatalysis, and ultimately accelerate the development of more robust and cost-effective bio-based PET recycling methods.

Suggested Citation

  • Ren Wei & Peter Westh & Gert Weber & Lars M. Blank & Uwe T. Bornscheuer, 2025. "Standardization guidelines and future trends for PET hydrolase research," Nature Communications, Nature, vol. 16(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-60016-9
    DOI: 10.1038/s41467-025-60016-9
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