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Glucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell division

Author

Listed:
  • Hien Bui

    (University of Helsinki
    University of Helsinki)

  • Simon Andersson

    (University of Helsinki
    University of Helsinki)

  • Agustin Sola-Carvajal

    (Karolinska Institutet)

  • Tommaso Marchi

    (Lund University)

  • Eliisa Vähäkangas

    (University of Helsinki
    University of Helsinki
    University of Helsinki)

  • Minna Holopainen

    (University of Helsinki
    University of Helsinki)

  • Andrew H. House

    (University of Helsinki
    University of Helsinki)

  • Bohdana M. Rovenko

    (University of Helsinki
    University of Helsinki)

  • Johanna I. Englund

    (University of Helsinki)

  • Maria Kasper

    (Karolinska Institutet)

  • Emilia Kuuluvainen

    (University of Helsinki
    University of Helsinki)

  • Reijo Käkelä

    (University of Helsinki
    University of Helsinki)

  • Ville Hietakangas

    (University of Helsinki
    University of Helsinki)

  • Emma Niméus

    (Lund University
    Skåne University Hospital)

  • Pekka Katajisto

    (University of Helsinki
    University of Helsinki
    Karolinska Institutet)

Abstract

Selective inheritance of sub-cellular components has emerged as a mechanism guiding stem cell fate after asymmetric cell divisions. Peroxisomes play a crucial role in multiple metabolic processes such as fatty acid metabolism and reactive oxygen species detoxification, but the apportioning of peroxisomes during stem cell division remains understudied. Here, we develop a mouse model and labeling technique to follow the dynamics of distinct peroxisome age-classes, and find that old peroxisomes are inherited by the daughter cell retaining full stem cell potency in mammary and epidermal stem cell divisions. Old peroxisomes carry Glucose-6-phosphate-dehydrogenase, whose specific location on the peroxisomal membrane promotes stem cell function by facilitating peroxisomal ether lipid synthesis. Our study demonstrates age-selective apportioning of peroxisomes in vivo, and unveils how functional heterogeneity of peroxisomes is utilized by asymmetrically dividing cells to metabolically divert the fate of the two daughter cells.

Suggested Citation

  • Hien Bui & Simon Andersson & Agustin Sola-Carvajal & Tommaso Marchi & Eliisa Vähäkangas & Minna Holopainen & Andrew H. House & Bohdana M. Rovenko & Johanna I. Englund & Maria Kasper & Emilia Kuuluvain, 2025. "Glucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell division," Nature Communications, Nature, vol. 16(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-58752-z
    DOI: 10.1038/s41467-025-58752-z
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    References listed on IDEAS

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