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Thy1 marks a distinct population of slow-cycling stem cells in the mouse epidermis

Author

Listed:
  • Elle Koren

    (Technion Israel Institute of Technology
    Technion Israel Institute of Technology
    Stanford University School of Medicine)

  • Alona Feldman

    (Technion Israel Institute of Technology
    Technion Israel Institute of Technology)

  • Marianna Yusupova

    (Technion Israel Institute of Technology
    Technion Israel Institute of Technology)

  • Avihay Kadosh

    (Technion Israel Institute of Technology)

  • Egor Sedov

    (Technion Israel Institute of Technology
    Technion Israel Institute of Technology)

  • Roi Ankawa

    (Technion Israel Institute of Technology
    Technion Israel Institute of Technology)

  • Yahav Yosefzon

    (Technion Israel Institute of Technology
    Technion Israel Institute of Technology)

  • Waseem Nasser

    (The Rappaport Faculty of Medicine and Research Institute, Technion Israel Institute of Technology)

  • Stefanie Gerstberger

    (Massachusetts General Hospital)

  • Liam B. Kimel

    (Technion Israel Institute of Technology)

  • Noa Priselac

    (Technion Israel Institute of Technology
    Technion Israel Institute of Technology)

  • Samara Brown

    (The Rockefeller University, New York)

  • Sam Sharma

    (The Rockefeller University, New York)

  • Travis Gorenc

    (The Rockefeller University, New York)

  • Ruby Shalom-Feuerstein

    (The Rappaport Faculty of Medicine and Research Institute, Technion Israel Institute of Technology)

  • Hermann Steller

    (The Rockefeller University, New York)

  • Tom Shemesh

    (Technion Israel Institute of Technology)

  • Yaron Fuchs

    (Technion Israel Institute of Technology
    Technion Israel Institute of Technology)

Abstract

The presence of distinct stem cells that maintain the interfollicular epidermis is highly debated. Here, we report a population of keratinocytes, marked by Thy1, in the basal layer of the interfollicular epidermis. We find that epidermal cells expressing differential levels of Thy1 display distinct transcriptional signatures. Thy1+ keratinocytes do not express T cell markers, express a unique transcriptional profile, cycle significantly slower than basal epidermal progenitors and display significant expansion potential in vitro. Multicolor lineage tracing analyses and mathematical modeling reveal that Thy1+ basal keratinocytes do not compete neutrally alike interfollicular progenitors and contribute long-term to both epidermal replenishment and wound repair. Importantly, ablation of Thy1+ cells strongly impairs these processes, thus indicating the non-redundant function of Thy1+ stem cells in the epidermis. Collectively, these results reveal a distinct stem cell population that plays a critical role in epidermal homeostasis and repair.

Suggested Citation

  • Elle Koren & Alona Feldman & Marianna Yusupova & Avihay Kadosh & Egor Sedov & Roi Ankawa & Yahav Yosefzon & Waseem Nasser & Stefanie Gerstberger & Liam B. Kimel & Noa Priselac & Samara Brown & Sam Sha, 2022. "Thy1 marks a distinct population of slow-cycling stem cells in the mouse epidermis," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31629-1
    DOI: 10.1038/s41467-022-31629-1
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    References listed on IDEAS

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