IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-48194-4.html
   My bibliography  Save this article

Impact of distinct FG nucleoporin repeats on Nup98 self-association

Author

Listed:
  • Alain Ibáñez de Opakua

    (German Center for Neurodegenerative Diseases (DZNE))

  • Christian F. Pantoja

    (German Center for Neurodegenerative Diseases (DZNE))

  • Maria-Sol Cima-Omori

    (German Center for Neurodegenerative Diseases (DZNE))

  • Christian Dienemann

    (Department of Molecular Biology)

  • Markus Zweckstetter

    (German Center for Neurodegenerative Diseases (DZNE)
    Department of NMR-based Structural Biology)

Abstract

Nucleoporins rich in phenylalanine/glycine (FG) residues form the permeability barrier within the nuclear pore complex and are implicated in several pathological cellular processes, including oncogenic fusion condensates. The self-association of FG-repeat proteins and interactions between FG-repeats play a critical role in these activities by forming hydrogel-like structures. Here we show that mutation of specific FG repeats of Nup98 can strongly decrease the protein’s self-association capabilities. We further present a cryo-electron microscopy structure of a Nup98 peptide fibril with higher stability per residue compared with previous Nup98 fibril structures. The high-resolution structure reveals zipper-like hydrophobic patches which contain a GLFG motif and are less compatible for binding to nuclear transport receptors. The identified distinct molecular properties of different regions of the nucleoporin may contribute to spatial variations in the self-association of FG-repeats, potentially influencing transport processes through the nuclear pore.

Suggested Citation

  • Alain Ibáñez de Opakua & Christian F. Pantoja & Maria-Sol Cima-Omori & Christian Dienemann & Markus Zweckstetter, 2024. "Impact of distinct FG nucleoporin repeats on Nup98 self-association," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48194-4
    DOI: 10.1038/s41467-024-48194-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-48194-4
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-48194-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Miao Yu & Maziar Heidari & Sofya Mikhaleva & Piau Siong Tan & Sara Mingu & Hao Ruan & Christopher D. Reinkemeier & Agnieszka Obarska-Kosinska & Marc Siggel & Martin Beck & Gerhard Hummer & Edward A. L, 2023. "Visualizing the disordered nuclear transport machinery in situ," Nature, Nature, vol. 617(7959), pages 162-169, May.
    2. Eszter E. Najbauer & Sheung Chun Ng & Christian Griesinger & Dirk Görlich & Loren B. Andreas, 2022. "Atomic resolution dynamics of cohesive interactions in phase-separated Nup98 FG domains," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    3. Jeong Hyun Ahn & Eric S. Davis & Timothy A. Daugird & Shuai Zhao & Ivana Yoseli Quiroga & Hidetaka Uryu & Jie Li & Aaron J. Storey & Yi-Hsuan Tsai & Daniel P. Keeley & Samuel G. Mackintosh & Ricky D. , 2021. "Phase separation drives aberrant chromatin looping and cancer development," Nature, Nature, vol. 595(7868), pages 591-595, July.
    4. Matthias Eibauer & Mauro Pellanda & Yagmur Turgay & Anna Dubrovsky & Annik Wild & Ohad Medalia, 2015. "Structure and gating of the nuclear pore complex," Nature Communications, Nature, vol. 6(1), pages 1-9, November.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Halima H. Schede & Pradeep Natarajan & Arup K. Chakraborty & Krishna Shrinivas, 2023. "A model for organization and regulation of nuclear condensates by gene activity," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Ruixue Xu & Lirong Lin & Zhiwei Jiao & Rui Liang & Yazhen Guo & Yixin Zhang & Xiaoxu Shang & Yuezhou Wang & Xu Wang & Luming Yao & Shengfa Liu & Xianming Deng & Jing Yuan & Xin-zhuan Su & Jian Li, 2024. "Deaggregation of mutant Plasmodium yoelii de-ubiquitinase UBP1 alters MDR1 localization to confer multidrug resistance," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    3. Di Yu & Yingying Liang & Claudia Kim & Anbalagan Jaganathan & Donglei Ji & Xinye Han & Xuelan Yang & Yanjie Jia & Ruirui Gu & Chunyu Wang & Qiang Zhang & Ka Lung Cheung & Ming-Ming Zhou & Lei Zeng, 2023. "Structural mechanism of BRD4-NUT and p300 bipartite interaction in propagating aberrant gene transcription in chromatin in NUT carcinoma," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    4. Xiuxiao Tang & Pengguihang Zeng & Kezhi Liu & Li Qing & Yifei Sun & Xinyi Liu & Lizi Lu & Chao Wei & Jia Wang & Shaoshuai Jiang & Jun Sun & Wakam Chang & Haopeng Yu & Hebing Chen & Jiaguo Zhou & Cheng, 2024. "The PTM profiling of CTCF reveals the regulation of 3D chromatin structure by O-GlcNAcylation," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    5. Sheung Chun Ng & Abin Biswas & Trevor Huyton & Jürgen Schünemann & Simone Reber & Dirk Görlich, 2023. "Barrier properties of Nup98 FG phases ruled by FG motif identity and inter-FG spacer length," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    6. Ashish Joshi & Anuja Walimbe & Anamika Avni & Sandeep K. Rai & Lisha Arora & Snehasis Sarkar & Samrat Mukhopadhyay, 2023. "Single-molecule FRET unmasks structural subpopulations and crucial molecular events during FUS low-complexity domain phase separation," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    7. Swarnendu Tripathi & Hazheen K. Shirnekhi & Scott D. Gorman & Bappaditya Chandra & David W. Baggett & Cheon-Gil Park & Ramiz Somjee & Benjamin Lang & Seyed Mohammad Hadi Hosseini & Brittany J. Pioso &, 2023. "Defining the condensate landscape of fusion oncoproteins," Nature Communications, Nature, vol. 14(1), pages 1-25, December.
    8. Mikhail E. Sushkin & Christine Koehler & Edward A. Lemke, 2023. "Remodeling the cellular stress response for enhanced genetic code expansion in mammalian cells," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48194-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.