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Loss-of-function of an α-SNAP gene confers resistance to soybean cyst nematode

Author

Listed:
  • Mariola Usovsky

    (University of Missouri)

  • Vinavi A. Gamage

    (University of Georgia)

  • Clinton G. Meinhardt

    (University of Missouri)

  • Nicholas Dietz

    (University of Missouri)

  • Marissa Triller

    (University of Missouri)

  • Pawan Basnet

    (University of Missouri)

  • Jason D. Gillman

    (University of Missouri)

  • Kristin D. Bilyeu

    (University of Missouri)

  • Qijian Song

    (Beltsville Agricultural Research Center)

  • Bishnu Dhital

    (University of Missouri)

  • Alice Nguyen

    (University of Missouri)

  • Melissa G. Mitchum

    (University of Georgia)

  • Andrew M. Scaboo

    (University of Missouri)

Abstract

Plant-parasitic nematodes are one of the most economically impactful pests in agriculture resulting in billions of dollars in realized annual losses worldwide. Soybean cyst nematode (SCN) is the number one biotic constraint on soybean production making it a priority for the discovery, validation and functional characterization of native plant resistance genes and genetic modes of action that can be deployed to improve soybean yield across the globe. Here, we present the discovery and functional characterization of a soybean resistance gene, GmSNAP02. We use unique bi-parental populations to fine-map the precise genomic location, and a combination of whole genome resequencing and gene fragment PCR amplifications to identify and confirm causal haplotypes. Lastly, we validate our candidate gene using CRISPR-Cas9 genome editing and observe a gain of resistance in edited plants. This demonstrates that the GmSNAP02 gene confers a unique mode of resistance to SCN through loss-of-function mutations that implicate GmSNAP02 as a nematode virulence target. We highlight the immediate impact of utilizing GmSNAP02 as a genome-editing-amenable target to diversify nematode resistance in commercially available cultivars.

Suggested Citation

  • Mariola Usovsky & Vinavi A. Gamage & Clinton G. Meinhardt & Nicholas Dietz & Marissa Triller & Pawan Basnet & Jason D. Gillman & Kristin D. Bilyeu & Qijian Song & Bishnu Dhital & Alice Nguyen & Meliss, 2023. "Loss-of-function of an α-SNAP gene confers resistance to soybean cyst nematode," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43295-y
    DOI: 10.1038/s41467-023-43295-y
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    References listed on IDEAS

    as
    1. Shiming Liu & Pramod K. Kandoth & Naoufal Lakhssassi & Jingwen Kang & Vincent Colantonio & Robert Heinz & Greg Yeckel & Zhou Zhou & Sadia Bekal & Johannes Dapprich & Bjorn Rotter & Silvia Cianzio & Me, 2017. "The soybean GmSNAP18 gene underlies two types of resistance to soybean cyst nematode," Nature Communications, Nature, vol. 8(1), pages 1-11, April.
    2. Ananda Y Bandara & Dilooshi K Weerasooriya & Carl A Bradley & Tom W Allen & Paul D Esker, 2020. "Dissecting the economic impact of soybean diseases in the United States over two decades," PLOS ONE, Public Library of Science, vol. 15(4), pages 1-28, April.
    3. Shiming Liu & Pramod K. Kandoth & Samantha D. Warren & Greg Yeckel & Robert Heinz & John Alden & Chunling Yang & Aziz Jamai & Tarik El-Mellouki & Parijat S. Juvale & John Hill & Thomas J. Baum & Silvi, 2012. "A soybean cyst nematode resistance gene points to a new mechanism of plant resistance to pathogens," Nature, Nature, vol. 492(7428), pages 256-260, December.
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