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Purkinje cell microzones mediate distinct kinematics of a single movement

Author

Listed:
  • François G. C. Blot

    (Erasmus MC)

  • Joshua J. White

    (Erasmus MC)

  • Amy van Hattem

    (Erasmus MC)

  • Licia Scotti

    (Erasmus MC)

  • Vaishnavi Balaji

    (Erasmus MC)

  • Youri Adolfs

    (University Medical Center Utrecht, Brain Center, Utrecht University)

  • R. Jeroen Pasterkamp

    (University Medical Center Utrecht, Brain Center, Utrecht University)

  • Chris I. De Zeeuw

    (Erasmus MC
    Royal Netherlands Academy of Arts and Sciences)

  • Martijn Schonewille

    (Erasmus MC)

Abstract

The classification of neuronal subpopulations has significantly advanced, yet its relevance for behavior remains unclear. The highly organized flocculus of the cerebellum, known to fine-tune multi-axial eye movements, is an ideal substrate for the study of potential functions of neuronal subpopulations. Here, we demonstrate that its recently identified subpopulations of 9+ and 9- Purkinje cells exhibit an intermediate Aldolase C expression and electrophysiological profile, providing evidence for a graded continuum of intrinsic properties among PC subpopulations. By identifying and utilizing two Cre-lines that genetically target these floccular domains, we show with high spatial specificity that these subpopulations of Purkinje cells participate in separate micromodules with topographically organized connections. Finally, optogenetic excitation of the respective subpopulations results in movements around the same axis in space, yet with distinct kinematic profiles. These results indicate that Purkinje cell subpopulations integrate in discrete circuits and mediate particular parameters of single movements.

Suggested Citation

  • François G. C. Blot & Joshua J. White & Amy van Hattem & Licia Scotti & Vaishnavi Balaji & Youri Adolfs & R. Jeroen Pasterkamp & Chris I. De Zeeuw & Martijn Schonewille, 2023. "Purkinje cell microzones mediate distinct kinematics of a single movement," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40111-5
    DOI: 10.1038/s41467-023-40111-5
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    References listed on IDEAS

    as
    1. Peter Thier & Peter W. Dicke & Roman Haas & Shabtai Barash, 2000. "Encoding of movement time by populations of cerebellar Purkinje cells," Nature, Nature, vol. 405(6782), pages 72-76, May.
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    3. David J. Herzfeld & Yoshiko Kojima & Robijanto Soetedjo & Reza Shadmehr, 2015. "Encoding of action by the Purkinje cells of the cerebellum," Nature, Nature, vol. 526(7573), pages 439-442, October.
    4. Kenneth D Harris & Hannah Hochgerner & Nathan G Skene & Lorenza Magno & Linda Katona & Carolina Bengtsson Gonzales & Peter Somogyi & Nicoletta Kessaris & Sten Linnarsson & Jens Hjerling-Leffler, 2018. "Classes and continua of hippocampal CA1 inhibitory neurons revealed by single-cell transcriptomics," PLOS Biology, Public Library of Science, vol. 16(6), pages 1-37, June.
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