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An optofluidic platform for interrogating chemosensory behavior and brainwide neural representation in larval zebrafish

Author

Listed:
  • Samuel K. H. Sy

    (The Chinese University of Hong Kong
    The Chinese University of Hong Kong
    The Chinese University of Hong Kong
    The University of Hong Kong)

  • Danny C. W. Chan

    (The Chinese University of Hong Kong
    The Chinese University of Hong Kong
    The Chinese University of Hong Kong
    The Chinese University of Hong Kong)

  • Roy C. H. Chan

    (The Chinese University of Hong Kong
    The Chinese University of Hong Kong)

  • Jing Lyu

    (The Chinese University of Hong Kong
    The Chinese University of Hong Kong)

  • Zhongqi Li

    (The Chinese University of Hong Kong
    The Chinese University of Hong Kong)

  • Kenneth K. Y. Wong

    (The University of Hong Kong
    Hong Kong Science Park)

  • Chung Hang Jonathan Choi

    (The Chinese University of Hong Kong
    The Chinese University of Hong Kong
    The Chinese University of Hong Kong)

  • Vincent C. T. Mok

    (The Chinese University of Hong Kong
    The Chinese University of Hong Kong
    The Chinese University of Hong Kong
    The Chinese University of Hong Kong)

  • Hei-Ming Lai

    (The Chinese University of Hong Kong
    The Chinese University of Hong Kong
    The Chinese University of Hong Kong
    The Chinese University of Hong Kong)

  • Owen Randlett

    (Université Claude Bernard Lyon 1)

  • Yu Hu

    (Hong Kong University of Science and Technology, Clear Water Bay, New Territories)

  • Ho Ko

    (The Chinese University of Hong Kong
    The Chinese University of Hong Kong
    The Chinese University of Hong Kong
    The Chinese University of Hong Kong)

Abstract

Studying chemosensory processing desires precise chemical cue presentation, behavioral response monitoring, and large-scale neuronal activity recording. Here we present Fish-on-Chips, a set of optofluidic tools for highly-controlled chemical delivery while simultaneously imaging behavioral outputs and whole-brain neuronal activities at cellular resolution in larval zebrafish. These include a fluidics-based swimming arena and an integrated microfluidics-light sheet fluorescence microscopy (µfluidics-LSFM) system, both of which utilize laminar fluid flows to achieve spatiotemporally precise chemical cue presentation. To demonstrate the strengths of the platform, we used the navigation arena to reveal binasal input-dependent behavioral strategies that larval zebrafish adopt to evade cadaverine, a death-associated odor. The µfluidics-LSFM system enables sequential presentation of odor stimuli to individual or both nasal cavities separated by only ~100 µm. This allowed us to uncover brainwide neural representations of cadaverine sensing and binasal input summation in the vertebrate model. Fish-on-Chips is readily generalizable and will empower the investigation of neural coding in the chemical senses.

Suggested Citation

  • Samuel K. H. Sy & Danny C. W. Chan & Roy C. H. Chan & Jing Lyu & Zhongqi Li & Kenneth K. Y. Wong & Chung Hang Jonathan Choi & Vincent C. T. Mok & Hei-Ming Lai & Owen Randlett & Yu Hu & Ho Ko, 2023. "An optofluidic platform for interrogating chemosensory behavior and brainwide neural representation in larval zebrafish," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-35836-2
    DOI: 10.1038/s41467-023-35836-2
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