IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-35536-3.html
   My bibliography  Save this article

Multimodal monitoring of human cortical organoids implanted in mice reveal functional connection with visual cortex

Author

Listed:
  • Madison N. Wilson

    (University of California San Diego)

  • Martin Thunemann

    (Boston University)

  • Xin Liu

    (University of California San Diego)

  • Yichen Lu

    (University of California San Diego)

  • Francesca Puppo

    (School of Medicine)

  • Jason W. Adams

    (School of Medicine
    University of California San Diego, School of Medicine)

  • Jeong-Hoon Kim

    (University of California San Diego)

  • Mehrdad Ramezani

    (University of California San Diego)

  • Donald P. Pizzo

    (University of California San Diego)

  • Srdjan Djurovic

    (Oslo University Hospital
    NORMENT Center
    University of Bergen
    University of Oslo)

  • Ole A. Andreassen

    (NORMENT Center
    University of Oslo
    Oslo University Hospital
    University of Oslo)

  • Abed AlFatah Mansour

    (The Salk Institute for Biological Studies
    The Hebrew University of Jerusalem)

  • Fred H. Gage

    (The Salk Institute for Biological Studies)

  • Alysson R. Muotri

    (School of Medicine
    University of California San Diego, School of Medicine
    University of California San Diego
    University of California San Diego)

  • Anna Devor

    (Boston University
    Massachusetts General Hospital)

  • Duygu Kuzum

    (University of California San Diego)

Abstract

Human cortical organoids, three-dimensional neuronal cultures, are emerging as powerful tools to study brain development and dysfunction. However, whether organoids can functionally connect to a sensory network in vivo has yet to be demonstrated. Here, we combine transparent microelectrode arrays and two-photon imaging for longitudinal, multimodal monitoring of human cortical organoids transplanted into the retrosplenial cortex of adult mice. Two-photon imaging shows vascularization of the transplanted organoid. Visual stimuli evoke electrophysiological responses in the organoid, matching the responses from the surrounding cortex. Increases in multi-unit activity (MUA) and gamma power and phase locking of stimulus-evoked MUA with slow oscillations indicate functional integration between the organoid and the host brain. Immunostaining confirms the presence of human-mouse synapses. Implantation of transparent microelectrodes with organoids serves as a versatile in vivo platform for comprehensive evaluation of the development, maturation, and functional integration of human neuronal networks within the mouse brain.

Suggested Citation

  • Madison N. Wilson & Martin Thunemann & Xin Liu & Yichen Lu & Francesca Puppo & Jason W. Adams & Jeong-Hoon Kim & Mehrdad Ramezani & Donald P. Pizzo & Srdjan Djurovic & Ole A. Andreassen & Abed AlFatah, 2022. "Multimodal monitoring of human cortical organoids implanted in mice reveal functional connection with visual cortex," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35536-3
    DOI: 10.1038/s41467-022-35536-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-35536-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-35536-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Giorgia Quadrato & Tuan Nguyen & Evan Z. Macosko & John L. Sherwood & Sung Min Yang & Daniel R. Berger & Natalie Maria & Jorg Scholvin & Melissa Goldman & Justin P. Kinney & Edward S. Boyden & Jeff W., 2017. "Cell diversity and network dynamics in photosensitive human brain organoids," Nature, Nature, vol. 545(7652), pages 48-53, May.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Harman Ghuman & Kyungsoo Kim & Sapeeda Barati & Karunesh Ganguly, 2023. "Emergence of task-related spatiotemporal population dynamics in transplanted neurons," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Anna Pagliaro & Roxy Finger & Iris Zoutendijk & Saskia Bunschuh & Hans Clevers & Delilah Hendriks & Benedetta Artegiani, 2023. "Temporal morphogen gradient-driven neural induction shapes single expanded neuroepithelium brain organoids with enhanced cortical identity," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    2. Elaine T. Lim & Yingleong Chan & Pepper Dawes & Xiaoge Guo & Serkan Erdin & Derek J. C. Tai & Songlei Liu & Julia M. Reichert & Mannix J. Burns & Ying Kai Chan & Jessica J. Chiang & Katharina Meyer & , 2022. "Orgo-Seq integrates single-cell and bulk transcriptomic data to identify cell type specific-driver genes associated with autism spectrum disorder," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    3. Yuge Wang & Hongyu Zhao, 2022. "Non-linear archetypal analysis of single-cell RNA-seq data by deep autoencoders," PLOS Computational Biology, Public Library of Science, vol. 18(4), pages 1-31, April.
    4. Yueqi Wang & Simone Chiola & Guang Yang & Chad Russell & Celeste J. Armstrong & Yuanyuan Wu & Jay Spampanato & Paisley Tarboton & H. M. Arif Ullah & Nicolas U. Edgar & Amelia N. Chang & David A. Harmi, 2022. "Modeling human telencephalic development and autism-associated SHANK3 deficiency using organoids generated from single neural rosettes," Nature Communications, Nature, vol. 13(1), pages 1-25, December.
    5. Tal Sharf & Tjitse Molen & Stella M. K. Glasauer & Elmer Guzman & Alessio P. Buccino & Gabriel Luna & Zhuowei Cheng & Morgane Audouard & Kamalini G. Ranasinghe & Kiwamu Kudo & Srikantan S. Nagarajan &, 2022. "Functional neuronal circuitry and oscillatory dynamics in human brain organoids," Nature Communications, Nature, vol. 13(1), pages 1-20, December.
    6. Maisumu Gulimiheranmu & Shuang Li & Junmei Zhou, 2021. "In Vitro Recapitulation of Neuropsychiatric Disorders with Pluripotent Stem Cells-Derived Brain Organoids," IJERPH, MDPI, vol. 18(23), pages 1-14, November.
    7. Alessandro Fiorenzano & Edoardo Sozzi & Marcella Birtele & Janko Kajtez & Jessica Giacomoni & Fredrik Nilsson & Andreas Bruzelius & Yogita Sharma & Yu Zhang & Bengt Mattsson & Jenny Emnéus & Daniella , 2021. "Single-cell transcriptomics captures features of human midbrain development and dopamine neuron diversity in brain organoids," Nature Communications, Nature, vol. 12(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35536-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.