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Activation of a transient progenitor state in the epicardium is required for zebrafish heart regeneration

Author

Listed:
  • Yu Xia

    (Weill Cornell Medical College
    Weill Cornell Medical College)

  • Sierra Duca

    (Weill Cornell Medical College
    Weill Cornell Medical College)

  • Björn Perder

    (Weill Cornell Medical College
    Weill Cornell Medical College)

  • Friederike Dündar

    (Weill Cornell Medical College
    Weill Cornell Medical College)

  • Paul Zumbo

    (Weill Cornell Medical College
    Weill Cornell Medical College)

  • Miaoyan Qiu

    (Weill Cornell Medical College
    Weill Cornell Medical College)

  • Jun Yao

    (Weill Cornell Medical College
    Weill Cornell Medical College)

  • Yingxi Cao

    (Weill Cornell Medical College
    Weill Cornell Medical College)

  • Michael R. M. Harrison

    (Weill Cornell Medical College
    Weill Cornell Medical College)

  • Lior Zangi

    (Icahn School of Medicine at Mount Sinai
    Icahn School of Medicine at Mount Sinai
    Icahn School of Medicine at Mount Sinai)

  • Doron Betel

    (Weill Cornell Medical College
    Weill Cornell Medical College
    Weill Cornell Medical College)

  • Jingli Cao

    (Weill Cornell Medical College
    Weill Cornell Medical College)

Abstract

The epicardium, a mesothelial cell tissue that encompasses vertebrate hearts, supports heart regeneration after injury through paracrine effects and as a source of multipotent progenitors. However, the progenitor state in the adult epicardium has yet to be defined. Through single-cell RNA-sequencing of isolated epicardial cells from uninjured and regenerating adult zebrafish hearts, we define the epithelial and mesenchymal subsets of the epicardium. We further identify a transiently activated epicardial progenitor cell (aEPC) subpopulation marked by ptx3a and col12a1b expression. Upon cardiac injury, aEPCs emerge from the epithelial epicardium, migrate to enclose the wound, undergo epithelial-mesenchymal transition (EMT), and differentiate into mural cells and pdgfra+hapln1a+ mesenchymal epicardial cells. These EMT and differentiation processes are regulated by the Tgfβ pathway. Conditional ablation of aEPCs blocks heart regeneration through reduced nrg1 expression and mesenchymal cell number. Our findings identify a transient progenitor population of the adult epicardium that is indispensable for heart regeneration and highlight it as a potential target for enhancing cardiac repair.

Suggested Citation

  • Yu Xia & Sierra Duca & Björn Perder & Friederike Dündar & Paul Zumbo & Miaoyan Qiu & Jun Yao & Yingxi Cao & Michael R. M. Harrison & Lior Zangi & Doron Betel & Jingli Cao, 2022. "Activation of a transient progenitor state in the epicardium is required for zebrafish heart regeneration," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35433-9
    DOI: 10.1038/s41467-022-35433-9
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    Cited by:

    1. Florian Constanty & Bailin Wu & Ke-Hsuan Wei & I-Ting Lin & Julia Dallmann & Stefan Guenther & Till Lautenschlaeger & Rashmi Priya & Shih-Lei Lai & Didier Y. R. Stainier & Arica Beisaw, 2025. "Border-zone cardiomyocytes and macrophages regulate extracellular matrix remodeling to promote cardiomyocyte protrusion during cardiac regeneration," Nature Communications, Nature, vol. 16(1), pages 1-25, December.
    2. Lei Li & Meina Lu & Lidong Guo & Xuejiao Zhang & Qun Liu & Meiling Zhang & Junying Gao & Mengyang Xu & Yijian Lu & Fang Zhang & Yao Li & Ruihua Zhang & Xiawei Liu & Shanshan Pan & Xianghui Zhang & Zhe, 2025. "An organ-wide spatiotemporal transcriptomic and cellular atlas of the regenerating zebrafish heart," Nature Communications, Nature, vol. 16(1), pages 1-20, December.

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