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Signal processing and generation of bioactive nitric oxide in a model prototissue

Author

Listed:
  • Songyang Liu

    (Hunan University)

  • Yanwen Zhang

    (Hunan University)

  • Xiaoxiao He

    (Hunan University)

  • Mei Li

    (University of Bristol)

  • Jin Huang

    (Hunan University)

  • Xiaohai Yang

    (Hunan University)

  • Kemin Wang

    (Hunan University)

  • Stephen Mann

    (University of Bristol
    Shanghai Jiao Tong University)

  • Jianbo Liu

    (Hunan University)

Abstract

The design and construction of synthetic prototissues from integrated assemblies of artificial protocells is an important challenge for synthetic biology and bioengineering. Here we spatially segregate chemically communicating populations of enzyme-decorated phospholipid-enveloped polymer/DNA coacervate protocells in hydrogel modules to construct a tubular prototissue-like vessel capable of modulating the output of bioactive nitric oxide (NO). By decorating the protocells with glucose oxidase, horseradish peroxidase or catalase and arranging different modules concentrically, a glucose/hydroxyurea dual input leads to logic-gate signal processing under reaction-diffusion conditions, which results in a distinct NO output in the internal lumen of the model prototissue. The NO output is exploited to inhibit platelet activation and blood clot formation in samples of plasma and whole blood located in the internal channel of the device, thereby demonstrating proof-of-concept use of the prototissue-like vessel for anticoagulation applications. Our results highlight opportunities for the development of spatially organized synthetic prototissue modules from assemblages of artificial protocells and provide a step towards the organization of biochemical processes in integrated micro-compartmentalized media, micro-reactor technology and soft functional materials.

Suggested Citation

  • Songyang Liu & Yanwen Zhang & Xiaoxiao He & Mei Li & Jin Huang & Xiaohai Yang & Kemin Wang & Stephen Mann & Jianbo Liu, 2022. "Signal processing and generation of bioactive nitric oxide in a model prototissue," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32941-6
    DOI: 10.1038/s41467-022-32941-6
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    References listed on IDEAS

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    1. Karina K. Nakashima & Merlijn H. I. Haren & Alain A. M. André & Irina Robu & Evan Spruijt, 2021. "Active coacervate droplets are protocells that grow and resist Ostwald ripening," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    2. Pierangelo Gobbo & Liangfei Tian & B. V. V. S Pavan Kumar & Samuel Turvey & Mattia Cattelan & Avinash J. Patil & Mauro Carraro & Marcella Bonchio & Stephen Mann, 2020. "Catalytic processing in ruthenium-based polyoxometalate coacervate protocells," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    3. Qingchuan Li & Shubin Li & Xiangxiang Zhang & Weili Xu & Xiaojun Han, 2020. "Programmed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    4. Carsten Donau & Fabian Späth & Marilyne Sosson & Brigitte A. K. Kriebisch & Fabian Schnitter & Marta Tena-Solsona & Hyun-Seo Kang & Elia Salibi & Michael Sattler & Hannes Mutschler & Job Boekhoven, 2020. "Active coacervate droplets as a model for membraneless organelles and protocells," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    5. Barbora Lavickova & Nadanai Laohakunakorn & Sebastian J. Maerkl, 2020. "A partially self-regenerating synthetic cell," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    6. Xin Huang & Mei Li & David C. Green & David S. Williams & Avinash J. Patil & Stephen Mann, 2013. "Interfacial assembly of protein–polymer nano-conjugates into stimulus-responsive biomimetic protocells," Nature Communications, Nature, vol. 4(1), pages 1-9, October.
    7. Alessandro Alcinesio & Oliver J. Meacock & Rebecca G. Allan & Carina Monico & Vanessa Restrepo Schild & Idil Cazimoglu & Matthew T. Cornall & Ravinash Krishna Kumar & Hagan Bayley, 2020. "Controlled packing and single-droplet resolution of 3D-printed functional synthetic tissues," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
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    1. Danping Tian & Ruipeng Hao & Xiaoming Zhang & Hu Shi & Yuwei Wang & Linfeng Liang & Haichao Liu & Hengquan Yang, 2023. "Multi-compartmental MOF microreactors derived from Pickering double emulsions for chemo-enzymatic cascade catalysis," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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