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Healthy cloned offspring derived from freeze-dried somatic cells

Author

Listed:
  • Sayaka Wakayama

    (University of Yamanashi
    University of Yamanashi)

  • Daiyu Ito

    (University of Yamanashi)

  • Erika Hayashi

    (University of Yamanashi)

  • Takashi Ishiuchi

    (University of Yamanashi)

  • Teruhiko Wakayama

    (University of Yamanashi
    University of Yamanashi)

Abstract

Maintaining biodiversity is an essential task, but storing germ cells as genetic resources using liquid nitrogen is difficult, expensive, and easily disrupted during disasters. Our aim is to generate cloned mice from freeze-dried somatic cell nuclei, preserved at −30 °C for up to 9 months after freeze drying treatment. All somatic cells died after freeze drying, and nucleic DNA damage significantly increased. However, after nuclear transfer, we produced cloned blastocysts from freeze-dried somatic cells, and established nuclear transfer embryonic stem cell lines. Using these cells as nuclear donors for re-cloning, we obtained healthy cloned female and male mice with a success rate of 0.2–5.4%. Here, we show that freeze-dried somatic cells can produce healthy, fertile clones, suggesting that this technique may be important for the establishment of alternative, cheaper, and safer liquid nitrogen-free bio-banking solutions.

Suggested Citation

  • Sayaka Wakayama & Daiyu Ito & Erika Hayashi & Takashi Ishiuchi & Teruhiko Wakayama, 2022. "Healthy cloned offspring derived from freeze-dried somatic cells," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31216-4
    DOI: 10.1038/s41467-022-31216-4
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    References listed on IDEAS

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    1. I. Wilmut & A. E. Schnieke & J. McWhir & A. J. Kind & K. H. S. Campbell, 1997. "Erratum: Viable offspring derived from fetal and adult mammalian cells," Nature, Nature, vol. 386(6621), pages 200-200, March.
    2. T. Wakayama & A. C. F. Perry & M. Zuccotti & K. R. Johnson & R. Yanagimachi, 1998. "Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei," Nature, Nature, vol. 394(6691), pages 369-374, July.
    3. I. Wilmut & A. E. Schnieke & J. McWhir & A. J. Kind & K. H. S. Campbell, 1997. "Viable offspring derived from fetal and adult mammalian cells," Nature, Nature, vol. 385(6619), pages 810-813, February.
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