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The importance of biofilm formation for cultivation of a Micrarchaeon and its interactions with its Thermoplasmatales host

Author

Listed:
  • Susanne Krause

    (Institute of Technology (KIT))

  • Sabrina Gfrerer

    (Institute of Technology (KIT)
    Institute of Technology (KIT))

  • Andriko Kügelgen

    (University of Oxford)

  • Carsten Reuse

    (Technische Universität Braunschweig
    Technische Universität Braunschweig)

  • Nina Dombrowski

    (NIOZ, Royal Netherlands Institute for Sea Research)

  • Laura Villanueva

    (NIOZ, Royal Netherlands Institute for Sea Research
    Utrecht University)

  • Boyke Bunk

    (Leibniz Institute DSMZ)

  • Cathrin Spröer

    (Leibniz Institute DSMZ)

  • Thomas R. Neu

    (Research UFZ)

  • Ute Kuhlicke

    (Research UFZ)

  • Kerstin Schmidt-Hohagen

    (Technische Universität Braunschweig
    Technische Universität Braunschweig)

  • Karsten Hiller

    (Technische Universität Braunschweig
    Technische Universität Braunschweig)

  • Tanmay A. M. Bharat

    (University of Oxford
    Structural Studies Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue)

  • Reinhard Rachel

    (University of Regensburg)

  • Anja Spang

    (NIOZ, Royal Netherlands Institute for Sea Research
    Uppsala University)

  • Johannes Gescher

    (Institute of Technology (KIT)
    Institute of Technology (KIT)
    Technical University of Hamburg)

Abstract

Micrarchaeota is a distinctive lineage assigned to the DPANN archaea, which includes poorly characterised microorganisms with reduced genomes that likely depend on interactions with hosts for growth and survival. Here, we report the enrichment of a stable co-culture of a member of the Micrarchaeota (Ca. Micrarchaeum harzensis) together with its Thermoplasmatales host (Ca. Scheffleriplasma hospitalis), as well as the isolation of the latter. We show that symbiont-host interactions depend on biofilm formation as evidenced by growth experiments, comparative transcriptomic analyses and electron microscopy. In addition, genomic, metabolomic, extracellular polymeric substances and lipid content analyses indicate that the Micrarchaeon symbiont relies on the acquisition of metabolites from its host. Our study of the cell biology and physiology of a Micrarchaeon and its host adds to our limited knowledge of archaeal symbioses.

Suggested Citation

  • Susanne Krause & Sabrina Gfrerer & Andriko Kügelgen & Carsten Reuse & Nina Dombrowski & Laura Villanueva & Boyke Bunk & Cathrin Spröer & Thomas R. Neu & Ute Kuhlicke & Kerstin Schmidt-Hohagen & Karste, 2022. "The importance of biofilm formation for cultivation of a Micrarchaeon and its interactions with its Thermoplasmatales host," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29263-y
    DOI: 10.1038/s41467-022-29263-y
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    References listed on IDEAS

    as
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