IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-27444-9.html
   My bibliography  Save this article

Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo

Author

Listed:
  • Yuki Bando

    (Columbia University
    Hamamatsu University School of Medicine)

  • Michael Wenzel

    (Columbia University
    University of Bonn)

  • Rafael Yuste

    (Columbia University)

Abstract

To better understand the input-output computations of neuronal populations, we developed ArcLight-ST, a genetically-encoded voltage indicator, to specifically measure subthreshold membrane potentials. We combined two-photon imaging of voltage and calcium, and successfully discriminated subthreshold inputs and spikes with cellular resolution in vivo. We demonstrate the utility of the method by mapping epileptic seizures progression through cortical circuits, revealing divergent sub- and suprathreshold dynamics within compartmentalized epileptic micronetworks. Two-photon, two-color imaging of calcium and voltage enables mapping of inputs and outputs in neuronal populations in living animals.

Suggested Citation

  • Yuki Bando & Michael Wenzel & Rafael Yuste, 2021. "Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27444-9
    DOI: 10.1038/s41467-021-27444-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-27444-9
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-27444-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Kiryl D. Piatkevich & Seth Bensussen & Hua-an Tseng & Sanaya N. Shroff & Violeta Gisselle Lopez-Huerta & Demian Park & Erica E. Jung & Or A. Shemesh & Christoph Straub & Howard J. Gritton & Michael F., 2019. "Population imaging of neural activity in awake behaving mice," Nature, Nature, vol. 574(7778), pages 413-417, October.
    2. Yoav Adam & Jeong J. Kim & Shan Lou & Yongxin Zhao & Michael E. Xie & Daan Brinks & Hao Wu & Mohammed A. Mostajo-Radji & Simon Kheifets & Vicente Parot & Selmaan Chettih & Katherine J. Williams & Benj, 2019. "Voltage imaging and optogenetics reveal behaviour-dependent changes in hippocampal dynamics," Nature, Nature, vol. 569(7756), pages 413-417, May.
    3. James F. A. Poulet & Carl C. H. Petersen, 2008. "Internal brain state regulates membrane potential synchrony in barrel cortex of behaving mice," Nature, Nature, vol. 454(7206), pages 881-885, August.
    4. Christopher J. Roome & Bernd Kuhn, 2018. "Simultaneous dendritic voltage and calcium imaging and somatic recording from Purkinje neurons in awake mice," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    5. Catherine A. Schevon & Shennan A. Weiss & Guy McKhann & Robert R. Goodman & Rafael Yuste & Ronald G. Emerson & Andrew J. Trevelyan, 2012. "Evidence of an inhibitory restraint of seizure activity in humans," Nature Communications, Nature, vol. 3(1), pages 1-11, January.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Eric Lowet & Krishnakanth Kondabolu & Samuel Zhou & Rebecca A. Mount & Yangyang Wang & Cara R. Ravasio & Xue Han, 2022. "Deep brain stimulation creates informational lesion through membrane depolarization in mouse hippocampus," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    2. Changjia Cai & Johannes Friedrich & Amrita Singh & M Hossein Eybposh & Eftychios A Pnevmatikakis & Kaspar Podgorski & Andrea Giovannucci, 2021. "VolPy: Automated and scalable analysis pipelines for voltage imaging datasets," PLOS Computational Biology, Public Library of Science, vol. 17(4), pages 1-28, April.
    3. Laura R González-Ramírez & Omar J Ahmed & Sydney S Cash & C Eugene Wayne & Mark A Kramer, 2015. "A Biologically Constrained, Mathematical Model of Cortical Wave Propagation Preceding Seizure Termination," PLOS Computational Biology, Public Library of Science, vol. 11(2), pages 1-34, February.
    4. Ashok Litwin-Kumar & Anne-Marie M Oswald & Nathaniel N Urban & Brent Doiron, 2011. "Balanced Synaptic Input Shapes the Correlation between Neural Spike Trains," PLOS Computational Biology, Public Library of Science, vol. 7(12), pages 1-14, December.
    5. Jianian Lin & Zongyue Cheng & Guang Yang & Meng Cui, 2022. "Optical gearbox enabled versatile multiscale high-throughput multiphoton functional imaging," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    6. Laurent Sheybani & Umesh Vivekananda & Roman Rodionov & Beate Diehl & Fahmida A. Chowdhury & Andrew W. McEvoy & Anna Miserocchi & James A. Bisby & Daniel Bush & Neil Burgess & Matthew C. Walker, 2023. "Wake slow waves in focal human epilepsy impact network activity and cognition," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    7. Suseendrakumar Duraivel & Shervin Rahimpour & Chia-Han Chiang & Michael Trumpis & Charles Wang & Katrina Barth & Stephen C. Harward & Shivanand P. Lad & Allan H. Friedman & Derek G. Southwell & Saurab, 2023. "High-resolution neural recordings improve the accuracy of speech decoding," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    8. Adrián Ponce-Alvarez & Biyu J He & Patric Hagmann & Gustavo Deco, 2015. "Task-Driven Activity Reduces the Cortical Activity Space of the Brain: Experiment and Whole-Brain Modeling," PLOS Computational Biology, Public Library of Science, vol. 11(8), pages 1-26, August.
    9. Amelie C. F. Bergs & Jana F. Liewald & Silvia Rodriguez-Rozada & Qiang Liu & Christin Wirt & Artur Bessel & Nadja Zeitzschel & Hilal Durmaz & Adrianna Nozownik & Holger Dill & Maëlle Jospin & Johannes, 2023. "All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    10. Annika Hagemann & Jens Wilting & Bita Samimizad & Florian Mormann & Viola Priesemann, 2021. "Assessing criticality in pre-seizure single-neuron activity of human epileptic cortex," PLOS Computational Biology, Public Library of Science, vol. 17(3), pages 1-18, March.
    11. Moritz Helias & Tom Tetzlaff & Markus Diesmann, 2014. "The Correlation Structure of Local Neuronal Networks Intrinsically Results from Recurrent Dynamics," PLOS Computational Biology, Public Library of Science, vol. 10(1), pages 1-21, January.
    12. Cheng Ly & Brent Doiron, 2009. "Divisive Gain Modulation with Dynamic Stimuli in Integrate-and-Fire Neurons," PLOS Computational Biology, Public Library of Science, vol. 5(4), pages 1-12, April.
    13. Yide Zhang & Binglin Shen & Tong Wu & Jerry Zhao & Joseph C. Jing & Peng Wang & Kanomi Sasaki-Capela & William G. Dunphy & David Garrett & Konstantin Maslov & Weiwei Wang & Lihong V. Wang, 2022. "Ultrafast and hypersensitive phase imaging of propagating internodal current flows in myelinated axons and electromagnetic pulses in dielectrics," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    14. Moritz Helias & Moritz Deger & Stefan Rotter & Markus Diesmann, 2010. "Instantaneous Non-Linear Processing by Pulse-Coupled Threshold Units," PLOS Computational Biology, Public Library of Science, vol. 6(9), pages 1-10, September.
    15. Christian Donner & Klaus Obermayer & Hideaki Shimazaki, 2017. "Approximate Inference for Time-Varying Interactions and Macroscopic Dynamics of Neural Populations," PLOS Computational Biology, Public Library of Science, vol. 13(1), pages 1-27, January.
    16. Sanaya N. Shroff & Eric Lowet & Sudiksha Sridhar & Howard J. Gritton & Mohammed Abumuaileq & Hua-An Tseng & Cyrus Cheung & Samuel L. Zhou & Krishnakanth Kondabolu & Xue Han, 2023. "Striatal cholinergic interneuron membrane voltage tracks locomotor rhythms in mice," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    17. Simone Carlo Surace & Jean-Pascal Pfister, 2015. "A Statistical Model for In Vivo Neuronal Dynamics," PLOS ONE, Public Library of Science, vol. 10(11), pages 1-21, November.
    18. Arita Silapetere & Songhwan Hwang & Yusaku Hontani & Rodrigo G. Fernandez Lahore & Jens Balke & Francisco Velazquez Escobar & Martijn Tros & Patrick E. Konold & Rainer Matis & Roberta Croce & Peter J., 2022. "QuasAr Odyssey: the origin of fluorescence and its voltage sensitivity in microbial rhodopsins," Nature Communications, Nature, vol. 13(1), pages 1-20, December.
    19. Iris Reuveni & Sourav Ghosh & Edi Barkai, 2017. "Real Time Multiplicative Memory Amplification Mediated by Whole-Cell Scaling of Synaptic Response in Key Neurons," PLOS Computational Biology, Public Library of Science, vol. 13(1), pages 1-31, January.
    20. John-Sebastian Mueller & Fabio C. Tescarollo & Trong Huynh & Daniel A. Brenner & Daniel J. Valdivia & Kanyin Olagbegi & Sahana Sangappa & Spencer C. Chen & Hai Sun, 2023. "Ictogenesis proceeds through discrete phases in hippocampal CA1 seizures in mice," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27444-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.