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Exploiting a Y chromosome-linked Cas9 for sex selection and gene drive

Author

Listed:
  • Stephanie Gamez

    (University of California, San Diego
    Agragene Inc.)

  • Duverney Chaverra-Rodriguez

    (University of California, San Diego)

  • Anna Buchman

    (University of California, San Diego
    Verily Life Sciences)

  • Nikolay P. Kandul

    (University of California, San Diego)

  • Stelia C. Mendez-Sanchez

    (University of California, San Diego
    School of Chemistry, Universidad Industrial de Santander)

  • Jared B. Bennett

    (Biophysics Graduate Group, University of California
    School of Public Health, University of California)

  • Héctor M. Sánchez C.

    (School of Public Health, University of California)

  • Ting Yang

    (University of California, San Diego)

  • Igor Antoshechkin

    (California Institute of Technology)

  • Jonny E. Duque

    (University of California, San Diego
    Universidad Industrial de Santander, Piedecuesta)

  • Philippos A. Papathanos

    (Food and Environment, Hebrew University of Jerusalem)

  • John M. Marshall

    (School of Public Health, University of California
    University of California)

  • Omar S. Akbari

    (University of California, San Diego)

Abstract

CRISPR-based genetic engineering tools aimed to bias sex ratios, or drive effector genes into animal populations, often integrate the transgenes into autosomal chromosomes. However, in species with heterogametic sex chromsomes (e.g. XY, ZW), sex linkage of endonucleases could be beneficial to drive the expression in a sex-specific manner to produce genetic sexing systems, sex ratio distorters, or even sex-specific gene drives, for example. To explore this possibility, here we develop a transgenic line of Drosophila melanogaster expressing Cas9 from the Y chromosome. We functionally characterize the utility of this strain for both sex selection and gene drive finding it to be quite effective. To explore its utility for population control, we built mathematical models illustrating its dynamics as compared to other state-of-the-art systems designed for both population modification and suppression. Taken together, our results contribute to the development of current CRISPR genetic control tools and demonstrate the utility of using sex-linked Cas9 strains for genetic control of animals.

Suggested Citation

  • Stephanie Gamez & Duverney Chaverra-Rodriguez & Anna Buchman & Nikolay P. Kandul & Stelia C. Mendez-Sanchez & Jared B. Bennett & Héctor M. Sánchez C. & Ting Yang & Igor Antoshechkin & Jonny E. Duque &, 2021. "Exploiting a Y chromosome-linked Cas9 for sex selection and gene drive," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27333-1
    DOI: 10.1038/s41467-021-27333-1
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    References listed on IDEAS

    as
    1. Nikolay P. Kandul & Junru Liu & Alexander D. Hsu & Bruce A. Hay & Omar S. Akbari, 2020. "A drug-inducible sex-separation technique for insects," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    2. Nikolay P. Kandul & Junru Liu & Hector M. Sanchez C. & Sean L. Wu & John M. Marshall & Omar S. Akbari, 2019. "Transforming insect population control with precision guided sterile males with demonstration in flies," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    3. Guan-Hong Wang & Stephanie Gamez & Robyn R. Raban & John M. Marshall & Luke Alphey & Ming Li & Jason L. Rasgon & Omar S. Akbari, 2021. "Combating mosquito-borne diseases using genetic control technologies," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    4. Ming Li & Ting Yang & Michelle Bui & Stephanie Gamez & Tyler Wise & Nikolay P. Kandul & Junru Liu & Lenissa Alcantara & Haena Lee & Jyotheeswara R. Edula & Robyn Raban & Yinpeng Zhan & Yijin Wang & Ni, 2021. "Suppressing mosquito populations with precision guided sterile males," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    5. Roberto Galizi & Lindsey A. Doyle & Miriam Menichelli & Federica Bernardini & Anne Deredec & Austin Burt & Barry L. Stoddard & Nikolai Windbichler & Andrea Crisanti, 2014. "A synthetic sex ratio distortion system for the control of the human malaria mosquito," Nature Communications, Nature, vol. 5(1), pages 1-8, September.
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