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Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii

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  • Christopher T. Straub

    (North Carolina State University)

  • Piyum A. Khatibi

    (North Carolina State University
    Novozymes Biologicals, Inc.)

  • Jack P. Wang

    (North Carolina State University)

  • Jonathan M. Conway

    (North Carolina State University)

  • Amanda M. Williams-Rhaesa

    (University of Georgia)

  • Ilona M. Peszlen

    (North Carolina State University)

  • Vincent L. Chiang

    (North Carolina State University)

  • Michael W. W. Adams

    (University of Georgia)

  • Robert M. Kelly

    (North Carolina State University)

Abstract

Microbial fermentation of lignocellulosic biomass to produce industrial chemicals is exacerbated by the recalcitrant network of lignin, cellulose and hemicelluloses comprising the plant secondary cell wall. In this study, we show that transgenic poplar (Populus trichocarpa) lines can be solubilized without any pretreatment by the extreme thermophile Caldicellulosiruptor bescii that has been metabolically engineered to shift its fermentation products away from inhibitory organic acids to ethanol. Carbohydrate solubilization and conversion of unpretreated milled biomass is nearly 90% for two transgenic lines, compared to only 25% for wild-type poplar. Unexpectedly, unpretreated intact poplar stems achieved nearly 70% of the fermentation production observed with milled poplar as the substrate. The nearly quantitative microbial conversion of the carbohydrate content of unpretreated transgenic lignocellulosic biomass bodes well for full utilization of renewable biomass feedstocks.

Suggested Citation

  • Christopher T. Straub & Piyum A. Khatibi & Jack P. Wang & Jonathan M. Conway & Amanda M. Williams-Rhaesa & Ilona M. Peszlen & Vincent L. Chiang & Michael W. W. Adams & Robert M. Kelly, 2019. "Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii," Nature Communications, Nature, vol. 10(1), pages 1-6, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11376-6
    DOI: 10.1038/s41467-019-11376-6
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    Cited by:

    1. Yao, Fengpei & Shen, Fei & Wan, Xue & Hu, Changwei, 2020. "High yield and high concentration glucose production from corncob residues after tetrahydrofuran + H2O co-solvent pretreatment and followed by enzymatic hydrolysis," Renewable and Sustainable Energy Reviews, Elsevier, vol. 132(C).
    2. Wang, Youmei & Liu, Peng & Zhang, Guifen & Yang, Qiaomei & Lu, Jun & Xia, Tao & Peng, Liangcai & Wang, Yanting, 2021. "Cascading of engineered bioenergy plants and fungi sustainable for low-cost bioethanol and high-value biomaterials under green-like biomass processing," Renewable and Sustainable Energy Reviews, Elsevier, vol. 137(C).

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