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In vitro Bioassay of Allelopathic Activities of Soybean, and Three Isoflavones, Using Protoplast Co-culture Method with Digital Image Analysis

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  • Hamako Sasamoto
  • Yosuke Kobayashi
  • Tomoya Oyanagi
  • Yutaka Sasamoto

Abstract

Inhibitory allelopathic activities of dried leaves of potted plants and protoplasts of Glycine max (soybean) cv. Okuhara-wase were studied using two in vitro bioassay methods. Strong inhibition (90%) of growth of recipient lettuce root was obtained with 50 mg soybean leaves using the lettuce seedling growth test (sandwich method), and moderate inhibition (65%) of lettuce protoplast division was obtained with 100 × 103 mL-1 protoplasts of etiolated soybean seedlings using the protoplast co-culture method with digital image analysis (DIA-PP method). Three isoflavones, genistein, genistin (genistein-7-O-glucoside), and daidzin (daidzein-7-O-glucoside) were investigated as putative allelochemicals using the DIA-PP method. The three isoflavones at 100 µM showed early crystal accumulation in lettuce protoplasts and inhibited lettuce protoplast division by 100%. Genistein showed the strongest inhibition. The results were compared and discussed with the previous reports on the invader leguminous plant, Kudzu, and two isoflavones, daidzein and Kudzu-specific puerarin (daidzein-6-C-glucoside), and other allelopathic plants and their putative allelochemicals.

Suggested Citation

  • Hamako Sasamoto & Yosuke Kobayashi & Tomoya Oyanagi & Yutaka Sasamoto, 2022. "In vitro Bioassay of Allelopathic Activities of Soybean, and Three Isoflavones, Using Protoplast Co-culture Method with Digital Image Analysis," Journal of Plant Studies, Canadian Center of Science and Education, vol. 11(1), pages 1-19, December.
    • Handle: RePEc:ibn:jpsjnl:v:11:y:2022:i:1:p:19
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    JEL classification:

    • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
    • Z0 - Other Special Topics - - General

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